A study of the use of agglutinating sera and phage lysis in the classification of strains of <i>Pseudomonas æruginosa</i>

Gould, J. C.; McLeod, J. W.

doi:10.1002/path.1700790211

Cited by 32 publications

(11 citation statements)

References 28 publications

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“…All strains tested of the genus Pseudornonas were oxidase-positive, as found by Buttiaux & Gagnon (1958); however, in one strain of P. owalis the reaction was delayed. The observation of Gould & McLeod (1960) that young unpigmented growth of P . aemcginosa gave a more intense oxidase reaction than older colonies was confirmed, Kohler (1959) reported P .…”

Section: Gram-negative Organismsmentioning

confidence: 99%

The Oxidase Reaction as a Taxonomic Tool

Steel

1961

Journal of General Microbiology

160

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SUMMARYThe oxidase test of Kovacs (1956) was applied to 1660 strains of various genera, of which 210 gave a positive reaction and 64 gave a delayed positive reaction. Members of the Neisseriaceae and Pseudomonadaceae were generally oxidasepositive, whereas members of the Enterobacteriaceae and, with few exceptions, Gram-positive organisms, were oxidase-negative ; equivocal results were obtained in the Brucellaceae and the genus Bacillus. Kovacs test is simple and quickly performed, but very sensitive. Some taxonomic implications of the results are discussed.

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Section: Gram-negative Organismsmentioning

confidence: 99%

The Oxidase Reaction as a Taxonomic Tool

Steel

1961

Journal of General Microbiology

160

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“…Bacteriophage typing was carried out upon all coagulase-positive staphylococci, by the methods of Blair & Williams (1961). Phage typing of Pseudomonas pyocyanea was performed by the method of Gould & McLeod (1960). Disk-diffusion sensitivity tests were carried out on all the pathogenic bacteria isolated.…”

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confidence: 99%

The mechanism and prevention of cross-infection in dermatological wards

Selwyn

1965

J. Hyg.

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Much of the extensive work on hospital cross-infection has of necessity been opportunist associated with the occurrence of sporadic outbreaks of infection. Investigations concentrated on such outbreaks cannot give a true picture of the general problem, and the value of any counter-measures which may have been introduced is not easily assessed.A pilot study, carried out in a large dermatology department, revealed an ideal model for the planned, long-term investigation of hospital infection (Selwyn, 1963). An endemic situation was uncovered in which the extensive skin lesions of in-patients were readily colonized by the abundant pathogenic bacteria present in the environment. Many of the infected patients became in their turn prolific dispersers of pathogens. A detailed study of these processes was facilitated by the long periods of hospital treatment which many patients with skin diseases require. Further work was therefore carried out in this neglected field to investigate the dynamic relationship which exists between the bacterial flora of these patients and that of their environment. The effect of simple anti-bacterial measures upon this relationship was also studied during two of the four stages of the investigation. The principal aim here was to assess the relative importance of nasal carriers and patients with infected lesions in the aetiology of hospital infection. MATERIALS AND METHODS Sampling proceduresThe investigation was carried out over a 2-year period in three dermatological wards. These are housed in a relatively modern pavilion, within a large teaching hospital. The two main wards, situated on different floors, are 68 ft. long, 28 ft. wide and 12 ft. high; each contains sixteen beds. The third ward, containing eight beds, is 32 ft. long; the width and height are as in the main wards.The degree of bacterial contamination was measured on two days of the week at representative sites in each ward, and in the adjacent day-room and bathroom. Air counts were performed with pairs of 3-5 in. Petri dishes containing blood agar and 7 5 % salt-milk agar respectively. These were exposed for 2 hr. periods as 'settle plates' and during each period similar plates were exposed in a 'Casella' slit sampler (but this could not be operated in the bathrooms). Solid surfaces were examined by contact-transfer using self-adhesive cellulose tape in a similar manner to that used by Thomas (1961) for swabbing skin. Direct impressions

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“…Those developed here have not yet been compared with those developed and used in other laboratories (28,(38)(39)(40)(41). Moreover, it is recognized that phages of pseudomonas, as well demonstrated in this study, may be rather difficult to maintain in "pure" lines because of the frequency of lysogenization and the unstable lysogenic state of the bacterial hosts.…”

Section: Resultsmentioning

confidence: 99%