A study of the reproducibility of the MTT test

Wan, Hongxia; Williams, Rachel; Doherty, Patrick; Williams, D. F.

doi:10.1007/bf00053336

Cited by 65 publications

(40 citation statements)

References 6 publications

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“…This test also displayed a lack of reproducibility, as already reported by Wan et al (25), due to the insolubility of formazan, the product formed. The MTT test therefore requires a solubilization step to dissolve the product in an organic solvent, such as DMSO (26).…”

Section: Discussionsupporting

confidence: 63%

Comparison of Tetrazolium Salt Assays for Evaluation of Drug Activity against Leishmania spp

et al. 2014

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In French Guiana, leishmaniasis is an essentially cutaneous infection. It constitutes a major public health problem, with a real incidence of 0.2 to 0.3%. Leishmania guyanensis is the causal species most frequently encountered in French Guiana. The treatment of leishmaniasis is essentially drug based, but the therapeutic compounds available have major side effects (e.g., liver damage and diabetes) and must be administered parenterally or are costly. The efficacy of some of these agents has declined due to the emergence of resistance in certain strains of Leishmania. There is currently no vaccine against leishmaniasis, and it is therefore both necessary and urgent to identify new compounds effective against Leishmania. The search for new drugs requires effective tests for evaluations of the leishmanicidal activity of a particular molecule or extract. Microculture tetrazolium assays (MTAs) are colorimetric tests based on the use of tetrazolium salts. We compared the efficacies of three tetrazolium salts-3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8)-for quantification of the promastigotes of various species of Leishmania. We found that the capacity of Leishmania to metabolize a tetrazolium salt depended on the salt used and the species of Leishmania. WST-8 was the tetrazolium salt best metabolized by L. guyanensis and gave the best sensitivity.

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Section: Discussionsupporting

confidence: 63%

Comparison of Tetrazolium Salt Assays for Evaluation of Drug Activity against Leishmania spp

et al. 2014

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“…It has been recognized that features of the surface morphology of the implant biomaterials may affect the cell response. 33 Therefore, it can be concluded that the chemical and physical properties of the AAc-grafted PET surface, including charge, chemistry, wettability, and morphology, affect the platelet adhesion and activation.…”

Section: Platelet Adhesionmentioning

confidence: 99%

Platelet adhesion on laser‐induced acrylic acid–grafted polyethylene terephthalate

Mirzadeh

Dadsetan

Sharifi-Sanjani

2002

J of Applied Polymer Sci

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ABSTRACT:To improve blood compatibility, acrylic acid (AAc) was grafted onto a polyethylene terephtalate (PET) film surface using lasers. The PET surface was irradiated with a CO 2 pulsed laser, and then graft copolymerization was carried out in an aqueous solution of AAc in the presence of Mohr's salt. Different techniques such as attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements were used to characterize the modified PET surface. The ATR-FTIR spectra confirmed the creation of new functional groups on the PET surface, and contact angle measurements revealed that the hydrophilicity of the PET surface increased as a result of the AAc graft polymerization. The electron micrographs showed that the grafting changed the surface morphology of the PET film. To evaluate the blood compatibility in vitro, the number of platelets adhering to the modified PET surface was determined using lactate dehydrogenase (LDH) activity measurement. The data from LDH method indicated that the extent of platelet adherence on the unmodified PET was much higher than that on the AAc grafted PET. The morphology of adhered platelets on the PET surface was investigated by SEM. The results showed that platelet adhesion and activation onto the PET surface was reduced because of AAc graft polymerization.

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“…2.6 MTT assay According to the previous paper, 31) MTT assay was carried out. MTT solution was prepared by dissolution of 7.5 mg of the lyophilized MTT powder into 30 cm 3 of aMEM contain ing 10 vol÷ FBS.…”

Section: Characterization Of Porous Calcium Phosphatesmentioning

confidence: 99%

泡セラミックス法と水熱処理法を組み合わせた多孔質リン酸カルシウムの合成とその細胞応答性

Aoki

Yamaguchi

Nakahira

et al. 2004

J. Ceram. Soc. Japan

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AZ~bNX@AEM@ðgÝí¹½½E¿_JVEÌ¬AE »Ì×E«ÂØTîERûrYE½ ÖÞ EÀº åãåwYAEÈw¤C5670047 ïØsüäu 81 Þ sH|@ÛåwH|w¨¿HwÈC6068585 ss ¶ae¼PèäC¹¬Four porous calcium phosphates (HAp, HAp/bTCP, bTCP, and aTCP) were prepared using a ceramic foaming technique combined with a hydrothermal treatment. These porous calcium phosphates had enough pore size, high specific surface area, and high porosity (80÷) for the cell growth of osteoblastlike cells. The compositions of the porous calcium phosphates depended on the solution in hydrothermal treatment and the sintering temperature. The cell activities of MC3T3E1 cells on porous HAp, HAp/bTCP, and bTCP were similar to that of monolayer cultivation as a control in MTT assay, ALPase activities, and the cell density.

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A study of the reproducibility of the MTT test

Cited by 65 publications

References 6 publications

Comparison of Tetrazolium Salt Assays for Evaluation of Drug Activity against Leishmania spp

Comparison of Tetrazolium Salt Assays for Evaluation of Drug Activity against Leishmania spp

Platelet adhesion on laser‐induced acrylic acid–grafted polyethylene terephthalate

泡セラミックス法と水熱処理法を組み合わせた多孔質リン酸カルシウムの合成とその細胞応答性

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