A study of the non-covalent interaction between flavonoids and DNA triplexes by electrospray ionization mass spectrometry

Screening and investigation of triplex DNA binders from Stephania tetrandra S. Moore by a combination of peak area‐fading ultra high‐performance liquid chromatography with orbitrap mass spectrometry and optical spectroscopies

“…ESI-MS is an efficient method to investigate DNA-ligand interactions [24,25]. The results were further verified using the direct infusion ESI-MS method, as described previously [23].…”

Molecular aspects on the interaction of quercetin and its metal complexes with DNA

“…Competitive bindings study between quercetin and DNA using acridine orange (AO) as a DNA probe has been reported by Bi et al They showed the fluorescence quenching in the system of quercetin-DNA-AO and demonstrated that the binding constants Intercalation and electrostatic binding [9] Quercetin-La (III) complex ∼1.00 × 10 5 Intercalation [28] of the quercetin with DNA in the presence of AO were 3.19 × 10 4 . They believe that groove binding was much more reasonable, through which the quercetin interacted reversibly with DNA [24].…”

“…DNA interaction with small molecules such as flavonoids has been studied intensively, since these small molecules exhibit a high potential as chemotherapeutic drugs, mainly in anticancer chemotherapy. Small molecules can react with DNA via covalent or noncovalent interactions [9]. There are several sites in the DNA molecule where such binding can occur: (i) between two base pairs (full intercalation), (ii) in the minor groove, (iii) in the major groove, and (iv) on the outside of the helix such ad electrostatics binding [10].…”

Study on noncovalent complexes of alkaloids with DNA duplex using electrospray ionization mass spectrometry

“…A previous study in our laboratory has found six alkaloids that interact with human survivin promoter DNA sequences using electrospray ionization mass spectrometry (ESI‐MS). Actually, ESI‐MS has been used to evaluate binding affinities and selectivities of ligands for different DNA for more than ten years . Compared with spectroscopic techniques (absorption, fluorescence, linear dichroism, NMR), calorimetric or electrochemical techniques, ESI‐MS has proved to be a valid and quick analytical technique for examining drug/DNA complexes .…”