A study of the interactions of Hg(II) with T-T mispair containing hairpin loops

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

“…A specific signal is produced due to the structure's conformational change when the hybridization between the target and the loop region occurs. Similar MB-based biosensors have been largely applied to detect DNAs [109,110], microRNAs [111], proteins [112], metal ions [113][114][115], and pathogens/viruses [116].…”

Role of the backbone of nucleic acids in the stability of Hg2+-mediated canonical base pairs and thymine–thymine mispair: a DFT study

“…46 Experimental and computational reports on the UV-vis absorption spectrum of TT dimers with Hg 2+ are available, which appears at 260 nm with the successive addition of mercury ions. 38,61,76 The mercury-mediated formation of TT dimers in DNA duplexes causes a decrease in the optical density with an increase in the Hg 2+ ion concentration in the different model systems. We examined the UV-vis absorption spectra using time-dependent density functional theory (TD-DFT) calculations in the aqueous phase (Fig.…”

A Multifunctional Molecular Probe for Detecting Hg2+ and Ag+ Based on Ion-Mediated Base Mismatch

“…Since Miyake et al demonstrated that Hg 2+ can bind and stabilize two thymine bases by forming a T–Hg 2+ –T binding mismatch, and Ag + is able to specifically interact with the C–Ag + –C mismatch in a DNA duplex [ 29 , 30 , 31 ], many researchers have applied these properties to the detection of Hg 2+ and Ag + in water and the environment. On this basis, many new methods for detecting Hg 2+ and Ag + have been proposed, such as the G-quadruplexes detection method [ 32 , 33 ], the fluorescence detection method [ 34 , 35 ], the electrochemical detection method [ 11 , 36 , 37 , 38 , 39 ], the DNA-modified gold nanoparticles detection method [ 11 , 16 , 21 , 40 ], the colorimetry detection method [ 41 , 42 ] and the probe microscope detection method [ 43 ]. These methods have the advantages of low cost, high efficiency, time efficiency, simple operation, high sensitivity, and specificity, and they completely overcome the disadvantages of traditional detection methods [ 44 ].…”