A study of the influence of the growth media on the fatty acid composition inCandida lipolytica diddens and lodder

Montēt, Didier; Ratomahenina, R.; Galzy, P.; Pina, Michel; Graille, Jean

doi:10.1007/bf01032285

Cited by 41 publications

(38 citation statements)

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“…(Candida) lipolytica strain cultured on vegetable oils lowered the α-linolenic acid concentration in its storage lipid [16]. The C. lipolytica strain used by Montet et al [11] showed significant ∆ 9 desaturase activity on accumulated C16:0, whereas erucic acid was shortened to oleic acid. In a similar manner, when Mucor circinelloides CBS 172-27 was cultured on sunflower oil, during storage lipid breakdown unsaturated fatty acids were preferentially oxidized [6].…”

Section: Discussionmentioning

confidence: 97%

“…Yarrowia (Candida) lipolytica can be considered to be a model microorganism with the ability to modify fatty materials. Various strains of this yeast have been reported to synthesize significant quantities of lipid composed mainly of TAGs, after growth on either fatty materials [7,10,11,16], or on substrates inducing de novo TAG biosynthesis (e.g. industrial glycerol) [17].…”

Section: Introductionmentioning

confidence: 99%

“…Compositional modifications conducted in both fat medium and accumulated lipid, may lead to the production of "new" lipids with industrial and financial importance (e.g. storage lipids mainly composed of TAGs either rich in polyunsaturated fatty acids or with structures similar to exotic fats) [6,9,10], especially if various crude fats or fatty waste-waters of low value (presently generated in high quantities [11][12][13][14][15]), could be used as substrate. Yarrowia (Candida) lipolytica can be considered to be a model microorganism with the ability to modify fatty materials.…”

Section: Introductionmentioning

confidence: 99%

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Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Papanikolaou

Aggelis

2003

Euro J Lipid Sci & Tech

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Yarrowia lipolytica ACA-DC 50109, maintained on potato dextrose agar (PDA, Fluka, Hannover, Germany) at T = 4 °C, was used. The salt composition of the culture medium was (g/l): KH 2 PO 4 7, Na 2 HPO 4 2. Selective uptake of fatty acids by the yeast Yarrowia lipolyticaYarrowia lipolytica, when cultivated on mixtures of free fatty acid substrates was found to remove C12:0, C14:0, ∆9 C18:1, ∆9,12 C18:2 and ∆9,12,15 C18:3 at significantly higher rates than C16:0 and C18:0, regardless of fatty acid composition of the initial substrate. C12:0, C14:0 and ∆9,12,15 C18:3 were specifically and completely removed from the substrate, while ∆9 C18:1 and ∆9,12 C18:2 concentrations decreased by 55-80 wt-% in comparison with initial concentrations. In contrast, concentration of C18:0 increased 2.1-3.5 fold in the substrate. Although C18:0 was removed slowly from the substrate, this fatty acid was selectively accumulated in the storage lipid. Inversely, only low quantities of ∆9 C18:1 and ∆9,12 C18:2 and traces of C12:0, C14:0 and ∆9,12,15 C18:3 were accumulated in the storage lipid. During storage lipid breakdown, cellular C16:0 and ∆9 C18:1 were taken up more rapidly than C18:0. We concluded that the capability of Yarrowia lipolytica to selectively remove several fatty acids from the substrate and accumulate others could be used in modification of the composition of selected mixtures of fatty acids and probably of common fats, to produce "new" fats with a predetermined composition.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Papanikolaou

Aggelis

2003

Euro J Lipid Sci & Tech

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“…[60,89,[94][95][96][97][98][99], fatty by-products or wastes such as crude fish oils, soap-stocks, ''stearin'' (a low-cost derivative of tallow composed of saturated free-fatty acids), hydrolyzed rapeseed oil, etc. [39,[100][101][102][103][104][105][106][107][108][109], pure freefatty acids [110][111][112], fatty esters [113], and n-alkanes [114,115]. In some investigations utilization of mixtures of hydro-…”

Section: Ex Novo Lipid Accumulationmentioning

confidence: 99%

Lipids of oleaginous yeasts. Part II: Technology and potential applications

Papanikolaou

Aggelis

2011

Euro J Lipid Sci & Tech

344

281

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The process of lipid accumulation in the oleaginous yeasts cultivated in various fermentation configurations when either sugars and related compounds or hydrophobic substances are used as substrates is presented and kinetic models describing both de novo and ex novo lipid accumulation are analyzed. Technological aspects related with single cell oil (SCO) produced by oleaginous yeasts are depicted. The influence of culture parameters upon lipid production process is presented. Lipid production has been studied in batch, fed-batch, and continuous cultivation systems using yeasts belonging to the species Lipomyces starkeyi, Rhodosporidium toruloides, Apiotrichum curvatum, Candida curvata, Cryptococcus curvatus, Trichosporon fermentans, and Yarrowia lipolytica. The potentiality of yeasts to produce SCO as starting material of 2nd generation biodiesel is indicated and discussed. Of significant importance is also the utilization of yeast lipids as substitutes of high added value exotic fats (e.g., cocoa butter). Lipid produced by the various yeasts presents, in general, similar composition with that of common vegetable oils being composed of unsaturated fatty acids, whereas cocoa butter is principally composed of saturated fatty acids, consequently the various strategies that are followed in order to increase the cellular saturated fatty acid content of the yeast lipid are presented and comprehensively discussed.

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“…Harvested cells were washed twice with either aqueous BSA (5 g/liter) (Sigma-Aldrich, St. Louis, Mo.) or hexane (20), giving rise to fractions containing SurfLip. InLip was extracted (10) from cells washed with SB buffer (9 g of NaCl/liter plus 5 g of BSA/liter), and LBLip was extracted from isolated LBs.…”

Section: Methodsmentioning

confidence: 99%

Lipid Accumulation, Lipid Body Formation, and Acyl Coenzyme A Oxidases of the Yeast Yarrowia lipolytica

Mlı́čková

Roux

Athenstaedt

et al. 2004

Appl Environ Microbiol

195

135

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Yarrowia lipolytica contains five acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX5 genes, that catalyze the limiting step of peroxisomal ␤-oxidation. In this study, we analyzed morphological changes of Y. lipolytica growing in an oleic acid medium and the effect of POX deletions on lipid accumulation. Protrusions involved in the uptake of lipid droplets (LDs) from the medium were seen in electron micrographs of the surfaces of wild-type cells grown on oleic acid. The number of protrusions and surface-bound LDs increased during growth, but the sizes of the LDs decreased. The sizes of intracellular lipid bodies (LBs) and their composition depended on the POX genotype. Only a few, small, intracellular LBs were observed in the mutant expressing only Aox4p (⌬pox2 ⌬pox3 ⌬pox5), but strains expressing either Aox3p or both Aox3p and Aox4p had the same number of LBs as did the wild type. In contrast, strains expressing either Aox2p or both Aox2p and Aox4p formed fewer, but larger, LBs than did the wild type. The size of the LBs increased proportionately with the amount of triacylglycerols in the LBs of the mutants. In summary, Aox2p expression regulates the size of cellular triacylglycerol pools and the size and number of LBs in which these fatty acids accumulate.The yeast Yarrowia lipolytica can grow on alkanes or fatty acids as the sole carbon source (for a review, see reference 5). Alkanes must be converted to fatty acids before they can serve as substrates for different metabolic pathways. In mammalian cells, the degradation of fatty acids occurs through ␤-oxidation in mitochondria and peroxisomes. In yeasts, the enzymes in this pathway are present only in peroxisomes. Fatty acid degradation is a multiple-step process requiring four different enzymatic activities. The first step is catalyzed by an acyl-coenzyme A (CoA) oxidase (Aoxp). Saccharomyces cerevisiae contains only one Aox (28), but Y. lipolytica contains five Aox isoenzymes with different substrate specificities and different activity levels ( Fig. 1) (27,28). Aox3p is specific for short-chain acyl-CoAs (17), Aox2p preferentially oxidizes long-chain acylCoAs (18), and the Aox4p and Aox5p activities do not appear to be sensitive to the length of the aliphatic chain of the acyl-CoA (29). Previous studies revealed that the mutants MTLY36 (⌬pox2 ⌬pox3 ⌬pox5) and MTLY37 (⌬pox2 ⌬pox3 ⌬pox4 ⌬pox5) either had a growth defect or did not grow at all, respectively, when cultivated on oleic acid minimal medium (28).Fatty acids can also be utilized as storage molecules when they are incorporated into triacylglycerols. All eukaryotic organisms and some gram-positive bacteria store triacylglycerols in intracellular compartments that are variously termed lipid particles, lipid droplets (LDs), lipid bodies (LBs), oil bodies, oleosomes, or spherosomes (in plants). The structure of these lipid-rich compartments is similar in all cell types and is rather simple: LBs consist of a hydrophobic core formed from neutral lipids, mainly triacylglycerols and/or steryl esters...

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A study of the influence of the growth media on the fatty acid composition inCandida lipolytica diddens and lodder

Cited by 41 publications

References 2 publications

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Selective uptake of fatty acids by the yeast Yarrowia lipolytica

Lipids of oleaginous yeasts. Part II: Technology and potential applications

Lipid Accumulation, Lipid Body Formation, and Acyl Coenzyme A Oxidases of the Yeast Yarrowia lipolytica

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