A study of the effects of column porosity on gradient separations of proteins

Shell particles, trials, tribulations and triumphs

“…This translated into minimum values of 2.6 for the former and 3.2 for the latter [72], showing a significant but moderate improvement. In a systematic comparison, Urban et al [74] found that the Poroshell 300 column performed better for the separation of proteins than columns packed with fully porous particles. It seems that the actual performance of the Poroshell columns was markedly improved between their initial development and their commercialization.…”

Monolithic Silica for Fast HPLC: Current Success and Promising Future

“…Both monolithic silica standard and narrowbore columns as special capillary LC monolithic silica columns (microseparation) have been used for fast separation and analysis of peptides and protein by mass spectrometry (MS) or by MS/MS [131][132][133][134][135][136][137][138][139][140][141].…”

Determination of PAHs by ultra fast liquid chromatography using a core-shell technology – Application to their determination after using biochar as adsorbent

“…The separation of PAHs by LC is frequently performed using columns packed with the octadecyl group bonded type silica gel (C 18 ) but in the last decades, the performance of the system was improved using ultra-high pressure liquid chromatography (UHPLC) system and columns packed with particles of sub-2-µm order, like pellicular and fused-core particles [8][9][10][11][12][13][14]. In the 1990s, a second generation of columns appeared [15] and more recently, columns packed with core-shell particles, called the third generation, were commercially available. The stationary phase particles of these columns are composed of a solid silica core surrounded by a homogeneous and durable porous layer, allowing better efficiencies than totally porous particles of the same size [8][9][10][11][12]16,17].…”

“…Efficiency and performance of these columns were improved with regard to each term of the Van Deemter plate height equation, i.e. HETP = A + B / u + C.u , where HETP is the height equivalent to a theoretical plate, u the linear velocity of the mobile phase and A, B, C the Eddy diffusion term, the longitudinal diffusion/acquity particles and the mass transfer coefficients, respectively [12][13][14][15][16][17]19]. The A parameter value, related to the flow of the mobile phase along the stationary phase, depends on the particle size.…”