A Study of the Effect of Zataria multiflora Extract on Methicillin Resistant Staphylococcus aureus

Motevasel, Maryam; Okhovat, Mohammad Ali; Zomorodian, Kamiar; Farshad, Shohreh

doi:10.5812/jjm.5453

Cited by 8 publications

(7 citation statements)

References 8 publications

(7 reference statements)

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“…Many minor skin infections (i.e., localized infections) caused by S. aureus can be treated with topical agents such as mupirocin and retapamulin; however, most MRSA strains are resistant to these and other conventional antibiotics. Cationic AMPs and their synthetic derivatives are promising candidates to combat antibiotic resistance ( 8 , 20 , 21 ). We investigated the wound-healing efficacy of CM11, a short hybrid cationic peptide, against S. aureus skin infection.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Healing Effect of Alginate Sulfate Hydrogel Dressing Containing Antimicrobial Peptide on Wound Infection Caused by Methicillin-Resistant Staphylococcus aureus

Babavalian

Latifi

Shokrgozar

et al. 2015

Jundishapur J Microbiol

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Background:Wound infections caused by methicillin-resistant Staphylococcus aureus are a health problem worldwide; therefore, it is necessary to develop new antimicrobial compounds. Considering broad-spectrum antimicrobial activity and low probability of drug resistance to peptides, applications these peptides are being studied extensively.Objectives:In this study, to control drug release over time, an alginate sulfate-based hydrogel impregnated with the CM11 peptide as the antimicrobial agent was developed, and its healing effects were tested on skin infections caused by methicillin-resistant S. aureus strains in a mouse model.Materials and Methods:Minimum inhibitory and minimum bactericidal concentrations of the CM11 peptide and alginate hydrogel in combination with the peptide were determined. Forty mice were divided into 4 groups: 1 group as a negative control (without treatment; however, 5 mice received hydrogel dressing without peptide), 1 group as a positive control (2% mupirocin treatment), and 2 groups as test groups. To establish skin infection, 200 μL of bacterial suspension with 3 × 108 CFU/mL concentration was subcutaneously injected in the scapular region of the mice. On the basis of the in vitro minimal bactericidal concentration of the alginate hydrogel containing peptide for 15 clinical isolates, hydrogel containing 128 mg/L of peptide was used for wound dressing over an 8-day period.Results:The highest and lowest numbers of wounds were observed on day 2 in the negative and positive control groups, respectively. During the 8-day period, the positive control and hydrogel containing peptide treatment groups showed similar levels of wound healing.Conclusions:This study showed that compared to standard drug treatment, treatment with hydrogel containing peptide had substantial antibacterial effects on S. aureus wound infections in mice.

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Section: Discussionmentioning

confidence: 99%

Analysis of Healing Effect of Alginate Sulfate Hydrogel Dressing Containing Antimicrobial Peptide on Wound Infection Caused by Methicillin-Resistant Staphylococcus aureus

Babavalian

Latifi

Shokrgozar

et al. 2015

Jundishapur J Microbiol

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“…ZM is chemically similar to T. vulgaris, and its leaf is listed in the Herbal Pharmacopoeia (15). Many studies have demonstrated the antibacterial and anti-inflammatory effects of ZM extract (16)(17)(18)(19) though there are inconclusive results about ZM products used for the relief of gastrointestinal syndromes, few studies have been carried out on the antitussive effects of this indigenous plant (20,21).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Zataria multiflora Extract Syrup and Diphenhydramine in the Treatment of Common Cold-Induced Cough in Children: A Double-Blind, Randomized, Clinical Trial

Hosseini¹,

Hossein²,

Amanati³

et al. 2016

Arch Pediatr Infect Dis

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Background: Management of cold-associated cough is a challenging aspect of supportive care for the common cold for pediatricians and parents worldwide. Herbal compounds have traditionally been used for the treatment of cold-related cough. Among these compounds, Zataria multiflora (ZM) has been found to be effective for cough relief. Objectives: The aim of this study is to compare a thyme mixture with diphenhydramine in the treatment of cold-related cough in a double-blind, randomized, clinical trial. Patients and Methods: A total of 52 pediatric patients (2 -12 years old) with common colds were included in the study. The patients were randomly divided into two treatment groups: one group received diphenhydramine compound and the other received ZM syrup, each for five days. The severity of cold-related symptoms and the efficacy of each drug were determined seven days later by asking the parents to complete a prepared questionnaire. sedation, sleepiness, a four-point scale of cough status, and a two-point scale of consumption convenience were also evaluated in these questionnaires. Results: Our results showed that sedation and sleepiness occurred in 30.8% and 19.2% of the patients in the diphenhydramine and ZM groups, respectively (P = 0.54). Also, 65.4% and 84.6% of the patients in the diphenhydramine and ZM groups, respectively, reported convenient usage (P = 0.10). The patients who received ZM syrup had significantly better outcomes (P = 0.036). Conclusions: Herbal compounds, such as ZM mixtures, are acceptably efficacious in cough relief with fewer adverse effects than chemical compounds in the treatment of cold-related cough, especially in infants and younger children.

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“…We showed that the MIC of ZMB extract for the standard strain was 10 µg/mL and for the clinical isolate it was 25 µg/mL, as compared with commercial antibiotics (Table ). Similar results showed that the alcoholic extract of ZMB at low concentrations could inhibit the growth of gram‐positive bacteria . In kinetic assays, Montagu et al showed that carvacrol had a bacteriostatic effect at 3 hours and a bactericidal effect from 6 to 24 hours.…”

Section: Discussionmentioning

confidence: 73%

“…Similar results showed that the alcoholic extract of ZMB at low concentrations could inhibit the growth of gram-positive bacteria. 19,20 In kinetic assays, Montagu et al 21 showed that carvacrol had a bacteriostatic effect at 3 hours and a bactericidal effect from 6 to 24 hours. Furthermore, Eftekhar et al 22 reported that ZMB essential oil had considerable in vitro activity against the standard ATCC cultures, as well as the clinical isolates of K. pneumonia.…”

Section: Discussionmentioning

confidence: 99%

In vivo antibacterial activity of Zataria multiflora Boiss extract and its components, carvacrol, and thymol, against colistin‐resistant Acinetobacter baumannii in a pneumonic BALB/c mouse model

Hassannejad

Bahador

Rudbari

et al. 2019

J of Cellular Biochemistry

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Background Acinetobacter baumannii has emerged as a major cause of nosocomial infections. Various resistance mechanisms of A. baumannii against antibiotics have transformed it into a successful nosocomial pathogen. Because of the limited number of available antibiotics, we used a medicinal plant with an antibacterial effect. Zataria multiflora Boiss (ZMB) extract and its components were used for the treatment of pneumonic mice infected with A. baumannii. The biological effects of this extract and the regulation of the outer membrane protein A (ompA) gene were used in a mouse model. Methods A pneumonic mouse model was prepared using clinical and standard strains (1.5 × 108 colony‐forming units/mL) of A. baumannii. BALB/c mice groups were treated with a ZMB extract, carvacrol, thymol, and sensitive antibiotics. The lung tissues of the treated mice were cultured for 5 days and each day, bacterial clearance and the ompA gene expression were assessed by quantitative real‐time polymerase chain reaction. Results In the lung tissue culture of pneumonic mice infected with standard or clinical isolate, no colony was detected when treated with the ZMB extract after 2 and 3 days (P < 0.01), respectively. In the carvacrol‐treated group, bacterial clearance was seen at day 4 and day 5 (P < 0.05). Bacterial clearance was seen 5 days after treatment with thymol and imipenem and 6 days after ampicillin/sulbactam treatment. The regulation of ompA gene was significantly decreased in this order: ZMB extract, carvacrol, thymol, imipenem, and ampicillin/sulbactam. Discussion The ZMB extract had a potent bactericidal effect against A. baumannii that could downregulate the ompA gene. ZBM extract and carvacrol could be novel therapeutic agents for antibiotic‐resistant A. baumannii.

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A Study of the Effect of Zataria multiflora Extract on Methicillin Resistant Staphylococcus aureus

Cited by 8 publications

References 8 publications

Analysis of Healing Effect of Alginate Sulfate Hydrogel Dressing Containing Antimicrobial Peptide on Wound Infection Caused by Methicillin-Resistant Staphylococcus aureus

Analysis of Healing Effect of Alginate Sulfate Hydrogel Dressing Containing Antimicrobial Peptide on Wound Infection Caused by Methicillin-Resistant Staphylococcus aureus

Comparison of Zataria multiflora Extract Syrup and Diphenhydramine in the Treatment of Common Cold-Induced Cough in Children: A Double-Blind, Randomized, Clinical Trial

In vivo antibacterial activity of Zataria multiflora Boiss extract and its components, carvacrol, and thymol, against colistin‐resistant Acinetobacter baumannii in a pneumonic BALB/c mouse model

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