A Study of the Cytotoxicity of a New Nonwoven Polymeric Fibrous Bandaging Material In-Vitro

Abstract

Traditionally used cotton-based bandaging materials have several disadvantages which can be overcome by using another fabric structure – nonwoven fabric. Moreover, these materials are more spongeous which increases their sorption capacity. The new bandaging material developed by the Institute of Physics of Strength and Material Science of the Siberian Branch of the Russian Academy of Sciences has even better sorption capacity with improved sorption properties. Its sorption capacity has been increased by means of an additional introduction of porous aluminum hydrate particles into fabric. It is important that a bandaging material has a good biocompatibility and does not have any cytotoxic effect on cells and tissues. Here it is present results of the study of the material's direct contact and indirect cytotoxicity assays in comparison with cotton gauze. It have found that in the direct contact of nonwoven polymeric fibrous bandaging material (NPFBM) with cells for 24 hours of cultivation no changes in cell morphology take place, nor does the amount of dead cells increase. These conclusions have been made by means of both a visual examination and an МТТ assay. The NPFBM extract did not have any cytotoxic effect on the tested cells either. The obtained results allow us to make a conclusion that the NPFBM complies with the international standard ISO 10993-5, which is applied to medical goods, and can from now on be applied in the treatment of infected wounds in clinical practice

Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects

“…Taking into account the diversity of medical devices and the complexity of the interaction between the medical devices and body, a uniform evaluation method or cytotoxicity test evaluation system cannot be established [38]. The cytotoxicity methods selected by us, such as the detection of cell damage by morphological changes (by F-actin staining), determination of cell damage (by lactate dehydrogenase release measurement), measuring cell growth and metabolic properties (by MTT or WST assay), represent the most commonly used tests for in vitro biocompatibility investigation of medical devices, including functionalized textiles, according to the literature available at this moment [39,40,41,42]. It is well known that there are three types of cytotoxicity tests mentioned in ISO 10993-5: extract, direct contact and indirect contact tests, the second one being the most sensitive for testing the cytotoxicity of the medical devices [38].…”

“…Also, the textile technologies have succeeded to bring novel advantages as researchers were able to develop biocompatible core-shell composite living fibers to act as scaffold for tissue engineering purposes [39]. Furthermore, fabrics without cytotoxic effects were developed for medical purposes, such as nonwoven polymeric fibrous bandaging materials for the treatment of infected wounds [40] and polypyrrole-coated polyester fabrics for vascular prostheses [42].…”