A Study of the Cell and Colony Variations of Blastomyces Dermatitidis

Kelley, William H.

doi:10.1093/infdis/64.3.293

Cited by 27 publications

(6 citation statements)

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“…The challenge inoculum of Paracoccidioides brasiliensis (Londofio) was grown in the yeast phase for 8 days on Kelly's medium [17] at 37 °C. Growth from three tubes was suspended in phosphate buffered saline and shaken for 5min on a vortex mixer.…”

Section: Intraperitoneal Inoculationmentioning

confidence: 99%

Pathogenesis and immune response toParacoccidioides brasiliensisin the fructivorous bat,Artibeus lituratus

Greer¹,

McMurray²

1981

Med Mycol

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Section: Intraperitoneal Inoculationmentioning

confidence: 99%

Pathogenesis and immune response toParacoccidioides brasiliensisin the fructivorous bat,Artibeus lituratus

Greer¹,

McMurray²

1981

Med Mycol

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“…However, standardized antigens and antisera are essential pre-requisites [2,6]. The mould-yeast conversion of B. dermatitidis in vitro is a fairly simple procedure on appropriate culture media at 37°C [1,3,7,10]. A widely recommended substrate for in vitro mould-yeast conversion of B. dermatitidis is pharmamedia [4,9,11] (Buckeye Cellulose Corporation, Memphis, Tennessee, and Traders Oil Mill Co., Traders Protein Division, Fort Worth, Texas, U.S.A.), a cottonseed embryo-derived proteinacious product.…”

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Cottonseed extract versus pharmamedia for thein vitromould-yeast conversion ofBlastomyces dermatitidis

et al. 1990

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A cottonseed medium, based on a 1% aqueous extract of seeds of any of the eight indigenously available varieties of cotton belonging to Gossypium hirsutum or Gossypium arboreum, was evaluated for the mould-yeast conversion of Blastomyces dermatitidis in vitro, and compared with pharmamedia agar as a control. The cottonseed agar was found to be as efficient as pharmamedia agar for the mould-yeast conversion of the 19 B. dermatitidis strains tested. Therefore, cottonseeds provide an adequate and inexpensive substitute for pharmamedia for the mould-yeast conversion of B. dermatitidis.It should be noted that the term 'cottonseed' medium has been used rather loosely in the literature and that this medium is based on pharmamedia and not on a cottonseed extract. The authors suggest that media based on pharmamedia should be referred to as pharrnamedia agar and the use of the term 'cottonseed' should be restricted to media containing cottonseed extract.Blastomyces dermatitidis, the etiologic agent of pulmonary and systemic blastomycosis in humans and canines, is generally isolated from clinical specimens in the mould form. As the mould form of the pathogen is not diagnostic, its identity in vitro is established by conversion to the yeast form or by the use of the exoantigen technique. The exoantigen test is performed by Ouchterlony's double diffusion technique and usually yields results within one week. However, standardized antigens and antisera are essential pre-requisites [2, 6]. The mould-yeast conversion of B. dermatitidis in vitro is a fairly simple procedure on appropriate culture media at 37°C [1, 3, 7, 10]. A widely recommended substrate for in vitro mould-yeast conversion of B. dermatitidis is pharmamedia [4,9,11] (Buckeye Cellulose Corporation, Memphis, Tennessee, and Traders Oil Mill Co., Traders Protein Division, Fort Worth, Texas, U.S.A.), a cottonseed embryo-derived proteinacious product. Recently, Kane [5] reported a Tween-albumin-niacin agar supplemented with casamino acid to be highly efficient for the mould-yeast conversion of B. dermatitidis. The preparation of pharmamedia glucose agar and Tween-albumin-niacin casamino acid agar media is

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“…At 370C, clinical isolates cultured on conventional conversion media occasionally require repeated subculture and often require extended incubation for conversion to the yeast form (5). Several agar media have been recommended for the conversion of B. dermatitidis from its mold form to its yeast forms, including brain heart infusion (BHI) agar, blood agar, blood agar supplemented by glutamine, Kelley agar, and cotton seed agar (1, 2,5,11,13,19,24). McGinnis has indicated that total colonial conversion is not necessary for the identification of B. dermatitidis (17).…”

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confidence: 99%

Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C

Kane¹

1984

J Clin Microbiol

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A Study of the Cell and Colony Variations of Blastomyces Dermatitidis

Cited by 27 publications

References 0 publications

Pathogenesis and immune response toParacoccidioides brasiliensisin the fructivorous bat,Artibeus lituratus

Pathogenesis and immune response toParacoccidioides brasiliensisin the fructivorous bat,Artibeus lituratus

Cottonseed extract versus pharmamedia for thein vitromould-yeast conversion ofBlastomyces dermatitidis

Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C

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