A Study of Interaction Between hWJ-MSCs and SiO₂-Coated PDMS Micropattern

Abstract

In order to enhance the likelihood of bone healing process from fracture, currently bone graft combined with osteocytes is a new alternative in promoting new bone formation. Silica-based nanoparticles had been proven to induce intrinsic biology activity, especially in promoting bone-formation and differentiation of mesenchymal stem cells towards osteocytes. Previous studies had shown positive results of new bone tissue formation by incorporating SiO2 nanoparticles synthesized using TEOS. In this current study, the experiment was using micropatterning technique, with PDMS as the substrate, which was coated by SiO2 nanoparticle powder with the particle size distribution between 150nm-450nm. The effect of SiO2-coated PDMS on (Wharton’s jelly mesenchymal stem cells) hWJ-MSCs viability was evaluated using MTT cytotoxicity assay for 1, 3, 5, and 7 days, which was compared with two treatments: cells grown with SiO2 powder without patterns and cells on 96 well plate). The MTT assay result showed that SiO2-coated PDMS micropattern was non-toxic to the cells and had the highest increase in cell viability for 7 days in comparison to the two controls used.