A Study of Imidazole-Based Nuclear Magnetic Resonance Probes of Cellular pH

Gasparovic, Charles; Barba, Ignasi; Born, Jan; Barton, Sandra L.; Arús, Carles; Mann, Paul L.

doi:10.1006/abio.1998.2698

Cited by 25 publications

(25 citation statements)

References 27 publications

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“…Histidine Administration and 1 5.88 (9). Using their titration results to calculate the brain pH values in our subjects yields an average pH of 6.85 Ϯ 0.05.…”

Section: Discussionmentioning

confidence: 99%

Administration and1H MRS detection of histidine in human brain: Application to in vivo pH measurement

2000

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“…Histidine Administration and 1 5.88 (9). Using their titration results to calculate the brain pH values in our subjects yields an average pH of 6.85 Ϯ 0.05.…”

Section: Discussionmentioning

confidence: 99%

Administration and1H MRS detection of histidine in human brain: Application to in vivo pH measurement

2000

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“…Using the imidazole-based NMR technique for pH measurement overcomes the broad band problem, however this method does not provide sufficient pH resolution (`1 ppm/per pH unit), and requires very good water suppression. 25 The proton resonances of TmDOTP 5À , on the other hand, have a strong chemical shift pH dependence ($3 ppm/pH unit) which is advantageous for clinical use.…”

Section: Tmdotpmentioning

confidence: 99%

“…16 Gasparolic et al utilized imidazole-based compounds as 1 H NMR probes with limited resolution for intracellular pH in perfused cells at high field, which requires highly effective water suppression. 25 Among the drawbacks of existing methodology is the fact that separate techniques are required to measure temperature and pH. Ideally, a single agent could be used for simultaneous measurements of both temperature and pH with high sensitivity in vivo.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous measurements of temperature and pHin vivo using NMR in conjunction with TmDOTP5?

et al. 2000

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NMR techniques for temperature and pH measurements have attracted increasing interest in recent years, motivated in part by the growing importance of medical hyperthermia for the treatment of cancer. The chemical shifts of thulium 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7, 10-tetrakis(methylene phosphonate) (TmDOTP5-) have been studied as a function of temperature and pH. The results demonstrate that TmDOTP5- resonance shifts are highly sensitive to temperature (approximately 1.0 ppm/degrees C) and pH (approximately 3.2 ppm/pH unit) at clinically relevant field strengths. A new method is presented which utilizes two magnetically non-equivalent protons in TmDOTP5- for simultaneous NMR measurements of both temperature and pH. The difference in the chemical shift values of pairs of 1H resonances provides a temperature sensitivity of about 1.6 ppm/ degrees C. The technique is demonstrated in live rats undergoing ultrasound-induced hyperthermia therapy.

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“…The addition of phosphate buffer (NaH 2 PO 4 / K 2 HPO 4 ) can be used to adjust the pH (~7.4) of the solution to minimize this effect. The property of pH sensitivity is usually employed to investigate the microenvironment of the cellparticularly that of the cancer cell [39,40]. In addition, different ionic concentrations of salt in solution (especially calcium and magnesium ions in urine) can cause the chemical shift to drift, which can be optimized by adding the appropriate potassium or sodium fluoride solution to decrease the concentration of Ca 2+ and Mg 2+ with ion exchange reactions [41,42].…”

Section: Nmr-based Metabolomic Approachmentioning

confidence: 99%

NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research

2016

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Purpose of Review In this review, the contribution of NMR to the metabolomics field will be discussed-particularly as it relates to cancer and drug metabolism research. An overview of the typical NMR metabolomic experiment will be presented while emphasizing important caveats ranging from experimental design to data interpretation. Recent Findings Despite some of the drawbacks of NMR, including reduced sensitivity compared to mass spectrometry-based approaches, NMR-based metabolomic approaches have provided considerable insight into drug and cancer metabolism. Advances in flux analysis based on the strategic use of isotopes have generated unique perspectives of cancer from the cellular and tissue levels. Summary Combined with other "omics" tools, including genomics, proteomics, and transcriptomics, metabolomic analysis completes the holistic view of metabolism. Many of these NMR-based approaches are likely to find their way into the clinic and become a common diagnostic tool.

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A Study of Imidazole-Based Nuclear Magnetic Resonance Probes of Cellular pH

Cited by 25 publications

References 27 publications

Administration and1H MRS detection of histidine in human brain: Application to in vivo pH measurement

Administration and1H MRS detection of histidine in human brain: Application to in vivo pH measurement

Simultaneous measurements of temperature and pHin vivo using NMR in conjunction with TmDOTP5?

NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research

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