A study of hydrolases stability in supercritical carbon dioxide (SC-CO2)

Enzymatic activity and conformational and morphological studies of four commercial lipases treated with supercritical carbon dioxide

“…These results are in agreement with Marty et al [27] that reported activity loss in the lipase from Mucor miehei treated with hydrated SC-CO 2 and no further activity loss in successive depressurizations using dry SC-CO 2 . It has been also reported that hydrolases with disulfide bonds have a lower degree of inactivation after several pressurization/depressurization cycles that enzymes without them [28]. 14 In this study, longer exposure times had greater negative effect on enzyme activity than the number of pressurization/depressurization cycles: for 60 min the activity loss was 5.6 % and 11.8% for Lipozyme RM IM and Lipozyme 435 respectively while for 360 min the activity loss increased up to 12.6% and 20.3 % for Lipozyme RM IM and Lipozyme 435, respectively.…”

Digestive enzymes characterization of krill (Euphausia superba) residues deoiled by supercritical carbon dioxide and organic solvents

“…The most important factor that may cause enzyme activity loss is probably the depressurization step; in a long-term enzyme application its activity decreases with increasing number of depressurizations [21].…”

Parameter optimization for the enzymatic hydrolysis of sunflower oil in high‐pressure reactors

“…Lipase-catalyzed reactions in SCF have been reported by numerous investigators (8)(9)(10). Supercritical media retain good enzyme activity and stability (11) and can be good solvents for hydrophobic compounds. An additional benefit of using enzymes in SCF is that it provides a very convenient way to recover products or nonreacted components.…”