A study of glycoprotein–lectin interactions using quartz crystal microbalance

Yakovleva, Maria; Safina, Gulnara; Danielsson, Bengt

doi:10.1016/j.aca.2009.12.004

Cited by 34 publications

(23 citation statements)

References 24 publications

(24 reference statements)

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“…A number of surfaces were functionalised with carbohydrates based on alpha‐D‐mannose, beta‐D‐galactose, and N‐acetyl‐beta‐D‐glucosamine and QCM was used to monitor the binding of several lectins with discreet selectivities (Norberg et al ., ). In another study, the lectins rather than the carbohydrates were bound to the sensor surface and used to detect glycoprotein interactions (Yakovleva et al ., ). In a similar way, lectin layers were prepared and used to bind glyconanoparticle sugar clusters (Mahon et al ., ) and glycovesicles (Mahon et al ., ).…”

Section: Carbohydratesmentioning

confidence: 97%

A Survey of the 2010 Quartz Crystal Microbalance Literature

Speight

Cooper

2012

J of Molecular Recognition

132

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In 2010 there has again been an increase in the number of papers published involving piezoelectric acoustic sensors, or quartz crystal microbalances (QCM), when compared to the last period reviewed 2006-2009. The average number of QCM publications per annum was 124 in the period 2001-2005, 223 in the period 2006-9, and 273 in 2010. There are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase), and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterisation of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights theoretical and practical aspects of the principals that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells, and membrane interfaces.

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Section: Carbohydratesmentioning

confidence: 97%

A Survey of the 2010 Quartz Crystal Microbalance Literature

Speight

Cooper

2012

J of Molecular Recognition

132

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“…A large variety of different biorecognition elements were tested for LPS biosensor applications [1,[8][9][10] including natural and synthetic proteins [11] and peptides, antibodies [12,13] and aptamers [14]. However, the most widely used biorecognition element in LPS biosensors is concanavalin A (Con A) [15][16][17][18][19][20][21]. It is lectin-type carbohydrate-binding protein obtained from jack beans (Canavalia ensiformis) with well-characterized structure that recognizes specifically α-Dglucose and α-D-mannose groups [9].…”

Section: Introductionmentioning

confidence: 99%

“…Interactions of Con A with analytes may be registered in a label-free mode using different experimental techniques, like surface plasmon resonance (SPR) [24,29,30], quartz crystal microbalance [15,[19][20][21]31] or electrochemical impedance on a Con A modified metal electrodes [12,[32][33][34][35]. Biosensors based on the last two techniques are the most widely reported.…”

Section: Introductionmentioning

confidence: 99%

Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration

Brosel-Oliu

Galyamin

Abramova

et al. 2017

Electrochimica Acta

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An impedimetric sensor based on a three dimensional electrode array modified with concanavalin A (Con A) was used for label-free detection of bacterial endotoxin: lipopolysaccharide (LPS) from Escherichia coli. The transducer permits the detection of the surface charge changes due to interaction of immobilized Con A biorecognition element and LPS of E. coli in test solution. The deposition of Con A on the surface was carried out using the layer-by-layer method with polyethyleneimine (PEI) polycation as an initial layer. The sensor surface characterization by means of electrochemical impedance spectroscopy technique allowed registering variations in superficial resistance provoked by surface charge changes and is demonstrated as an effective method to monitor sensor parameters at each modification step as well as to follow Con A-LPS reaction. In order to prevent nonspecific adsorption of LPS on PEI covered surface different blocking strategies were tested to achieve the specific response between Con A and LPS. Results obtained in this work clearly show that blocking with bovine serum albumin (BSA) is not sufficient to prevent non-specific interactions of PEI and to ensure the selective biorecognition of LPS by Con A. To achieve more efficient PEI blocking a new method was proposed based on consecutive deposition of Con A-glycogen-Con A layers. Sensors modified with PEI-(Con A-Gly)2-Con A multilayers are shown to be highly sensitive, selective and reproducible. Presented biosensor is able to detect bacterial LPS in a very short detection time (20 min) with 1.5 µg/mL limit of detection, which is much lower than reported for other biosensors with Con A. CENTRO NACIONAL DE MICROELECTRÓNICA INSTITUTO DE MICROELECTRÓNICA DE BARCELONA Dear editor, On behalf of the authors I would like to submit a manuscript: " Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration" based on the work carried out in

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“…The mainstream of recent QCM-based techniques is production of special coating layers for quartz plate, which are able to adsorb specifically target chemicals, and evaluate them quantitatively by quartz frequency shift [32] [33]. This approach, for example, allows Lin et al [34] albumin determination with detection limits of 60 -150 ppm, and Yakovleva et al [35] glycoproteins determinations from 50 µg/mL to 1 mg/mL. It was possible also to determine toxic components, and a number of specific antibodies in biological fluids, if a quartz plate coating contained immobilized specific antigens [36] [37].…”

Section: Introductionmentioning

confidence: 99%

Drying Drop Technology as a Possible Tool for Detection Leukemia and Tuberculosis in Cattle

Yakhno¹,

Sanin²,

Ilyazov³

et al. 2015

JBiSE

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Usual response of organism to viral or bacterial invasion represents antibodies production, qualitative and quantitative changes in composition of biological fluids. These changes influence conformation and surface characteristics of macromolecules (proteins), which become apparent in sessile drying drops, when they form aggregates due to salting-out effect and sediment. The bottom adsorption layers change their adhesive and viscoelastic properties in time depending on fluid composition and structure. The aim of this study was verification the idea of using this phenomenon in rapid vet diagnostics. Milk, blood and serum samples of 183 cows were tested using Drop Drying Technology (DDT). A drop of tested fluid dried on a polished quartz plate, oscillated with constant frequency-60 kHz. Mechanical properties of the drop changed during drying, influenced the electrical conductivity of the quartz plate. This signal was converted to the Acoustical-Mechanical Impedance (AMI) and displayed as a curve in coordinates AMI vs. Time. Shape of the AMI curve reflected this dynamics, and was used as a target for quantitative comparison between * Corresponding author. T. A. Yakhno et al. 2 control and infected animals. Frequency analysis of the estimated parameters of the curves was performed using features of the Excel program. Powerful method of artificial neural network processing of the experimental data was also tested in this work as a possible tool for future development. Significant differences between control, Bovine leucosis virus positive (BLV+) and Bovine tuberculin positive (BTub+) cattle groups were obtained using all biological fluids-blood, serum and milk. We fixed also a season shift of the data, but distinction between groups still remained. In serum and milk some features of the AMI curves were more stable, and retained diagnostic properties when combined winter and spring databases. Further development of DDT is proposed.

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A study of glycoprotein–lectin interactions using quartz crystal microbalance

Cited by 34 publications

References 24 publications

A Survey of the 2010 Quartz Crystal Microbalance Literature

A Survey of the 2010 Quartz Crystal Microbalance Literature

Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration

Drying Drop Technology as a Possible Tool for Detection Leukemia and Tuberculosis in Cattle

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