A Study of Factors Affecting the Success of Human Fertilization in Vitro. I. Influence of Ovarian Stimulation Upon the Number and Condition of Oocytes Collected

Testart, J; Frydman, René; Mouzon, J. de; Lassalle, Bruno; Belaïsch, J

doi:10.1095/biolreprod28.2.415

Cited by 70 publications

(21 citation statements)

References 10 publications

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“…For these calculations we did not consider oocytes classified as intermediate and the "gold standard" was microscopic analysis of the oocytes as described previously (10,11).…”

Section: Discussionmentioning

confidence: 99%

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Costa

Mendes

Ferriani

et al. 2004

Braz J Med Biol Res

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The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing mature oocytes (median = 368 and 5.7 ng/ml, respectively; P < 0.05). Progesterone, androstenedione and estrone levels were not significantly different between mature and immature oocytes. The application of the receiver-operating characteristic curve statistical approach to determine the best cut-off point for the discrimination between mature and immature oocytes indicated levels of 505.8 ng/ml for E2 (81.0% sensitivity and 81.8% specificity) and of 10.4 ng/ ml for testosterone (90.9% sensitivity and 82.4% specificity). Follicular diameter was associated negatively with E2 and testosterone levels in FF. There was a significant increase in progesterone/testosterone, progesterone/E2 and E2/testosterone ratios in FF containing mature oocytes, suggesting a reduction in conversion of C21 to C19, but not in aromatase activity. The overall fertility rate was 61% but there was no correlation between the steroid levels or their ratios and the fertilization rates. E2 and testosterone levels in FF may be used as a predictive parameter of oocyte maturity, but not for the in vitro fertilization rate.

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“…For these calculations we did not consider oocytes classified as intermediate and the "gold standard" was microscopic analysis of the oocytes as described previously (10,11).…”

Section: Discussionmentioning

confidence: 99%

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Costa

Mendes

Ferriani

et al. 2004

Braz J Med Biol Res

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show abstract

“…Culture of immature cumulus complexes in vitro can lead to the maturation of their oocytes (Testart et al, 1983b;Boldt et al, 1987). The proportion of immature oocytes recovered is affected by the method of ovarian stimulation, being © Oxford University Press higher with use of clomiphene citrate and/or human menopausal gonadotrophin (HMG) because of the more asynchronous follicular growth than is observed with use of gonadotrophin releasing hormone (GnRH) superactive analogues combined with HMG (Cabau and Bessis, 1981;Testart et al, 1983a). The number of immature oocytes can also be reduced if administration of human chorionic gonadotrophin (HCG) is delayed until more large follicles ( > 18 mm) are present.…”

Section: Can Failed-fertilized Oocytes Produce Pregnancies?mentioning

confidence: 99%

Are failed-fertilized human oocytes useful?

Winston

Braude

Johnson³

1993

Human Reproduction

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“…Since the introduction of IVF for clinical purposes, a considerable body of evidence has accumulated to suggest that in stimulated cycles the type of COH protocol used may affect the rate and synchrony of the maturation of oocytes aspirated. In menotropin-only stimulated IVF cycles, resumption of meiosis and expansion of the OCCC have been noted to be asynchronous (1,3,13,23,24). As noted, (1,3,8), only 39 to 55% of oocytes had a PB at the time of retrieval and sometimes even half still possessed a GV at retrieval.…”

Section: Discussionmentioning

confidence: 91%

Treatment variables in relation to oocyte maturation: Lessons from a clinical micromanipulation-assisted in vitro fertilization program

Avrech

Goldman

Rufas

et al. 1997

J Assist Reprod Genet

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A Study of Factors Affecting the Success of Human Fertilization in Vitro. I. Influence of Ovarian Stimulation Upon the Number and Condition of Oocytes Collected

Cited by 70 publications

References 10 publications

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Are failed-fertilized human oocytes useful?

Treatment variables in relation to oocyte maturation: Lessons from a clinical micromanipulation-assisted in vitro fertilization program

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