A study of degeneration and regeneration in the divided rat sciatic nerve based on electron microscopy

Morris, James H.; Hudson, Alan R.; Weddell, G.

doi:10.1007/bf00981944

Cited by 153 publications

(42 citation statements)

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“…This segregation is an obligatory antecedent to myelination (Morris et al, 1972;Webster, 1993); there are a few descriptions of myelination of more than one axon by the same Schwann cell, but these examples are rare (Waxman, 1968;Jaros and Bradley, 1979;Beuche and Friede, 1984). The process of segregation occurred in the GDNF-treated nerves in very much the same way as seen during development.…”

Section: Segregation To a 1:1 Relationshipmentioning

confidence: 98%

Glial Cell Line-Derived Neurotrophic Factor Alters Axon Schwann Cell Units and Promotes Myelination in Unmyelinated Nerve Fibers

et al. 2003

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Glial cell line-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of a subset of dorsal root ganglion sensory neurons. We administered high-dose exogenous recombinant human GDNF (rhGDNF) daily to adult rats to examine its effect on unmyelinated axon-Schwann cell units in intact peripheral nerves. In rhGDNF-treated animals, there was a dramatic proliferation in the Schwann cells of unmyelinated fibers, which resulted in the segregation of many unmyelinated axons into a 1:1 relationship with Schwann cells and myelination of normally unmyelinated small axons. This study demonstrates that the administration of high doses of a growth factor to adult rats can change the phenotype of nerve fibers from unmyelinated to myelinated.

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Section: Segregation To a 1:1 Relationshipmentioning

confidence: 98%

Glial Cell Line-Derived Neurotrophic Factor Alters Axon Schwann Cell Units and Promotes Myelination in Unmyelinated Nerve Fibers

et al. 2003

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“…These results are consistent with previous studies (Williams et al, 1983;Madison et al, 1988) suggested that activated macrophages can secrete numerous growth factors, including nerve growth factor (NGF) and basic fibroblast growth factor which can promote regeneration in transected nerves . (Morris et al, 1972;Williams et al, 1983;Knoops et al, 1990;Kosaka, 1990;Derby et al, 1993;Tong et al, 1994). For example, phosphate-buffered 2% osmic acid has been used to fix myelin sheaths efficiently in the case of myelinated axons found in studies using relatively larger inner diameter nerve cuff silicone rubber tubing (Woolley et al.…”

Section: Discussionmentioning

confidence: 99%

“…These axon-Schwann cluster formations are the common organizational structures seen under similar nerve cuff bridging conditions and such structures have been termed "regenerating units" by Morris et al (1972) in their study observing regeneration of divided rat peripheral nerve. Williams et al (1983) also found that many cells were organized in clusters at locations 1 -3 mm from the distal stump toward the midpoint of cables regenerated between proximal and distal stumps using silicone rubber single-lumen nen/e cuffs.…”

Section: Regeneration Units and Schwann Ceil Coiumnsmentioning

confidence: 99%

Development of a Multiple-Lumen Nerve Cuff Utilizing Growth Stimulant Patterns for Controlled Regeneration

et al. 1998

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This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.

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“…Compartmentation of the nerve fascicle has been observed in the regenerating process of the retrograde degenerated part of the transected proximal nerve stump and considered a mechanism for perineurial regeneration and reconstitution of the blood-nerve barrier. 16,17 The regenerating unit is considered to be a cluster of a multiple sprouts occurring from a single axon and extending along a single Schwann cell's basal lamina. 17 In our study, we further observed the appearance of a regenerating axon from the node of Ranvier in the longitudinal section.…”

Section: Stretching Of the Proximal Stump Induces The Growth Of Sproutsmentioning

confidence: 99%

“…16,17 The regenerating unit is considered to be a cluster of a multiple sprouts occurring from a single axon and extending along a single Schwann cell's basal lamina. 17 In our study, we further observed the appearance of a regenerating axon from the node of Ranvier in the longitudinal section. Furthermore, in the present study, the total number of myelinated fibers also increased near the marking suture.…”

Section: Stretching Of the Proximal Stump Induces The Growth Of Sproutsmentioning

confidence: 99%

Gradual stretching of the proximal nerve stump induces the growth of regenerating sprouts in rats

Saijilafu

Nishiura

Hara

et al. 2008

Journal Orthopaedic Research

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ABSTRACT:We investigated the effect of direct gradual stretching on the proximal nerve stump morphologically. A 10-mm-long nerve segment was resected from the sciatic nerve of a rat. The end of the proximal nerve stump was fixed to a small ring and the marking suture was placed at a point 1 mm proximal to the ring. Then, the nerve stump was lengthened at a rate of 1 mm/day via a stretching of the ring using an original external device. After a stretching of 20 days, the distance from the ring to the marking suture became 12 mm. Whereas large mature myelinated axons were observed in the proximal part of the marking, only small axons with thin myelin sheath were observed in the distal part, and the mean axonal diameter showed a significant difference between the two parts. Moreover, the mean internodal length was 172.4 AE 13.4 mm in the distal part of the marking and 1019.0 AE 56.2 mm in the proximal part. The internodal length also showed a significant difference between the two parts. Thus, the axonal diameter and internodal length were consistent with the characteristics of regenerating axons in the distal part. Furthermore, ultrastructural analysis also showed the histological characteristics of axonal regeneration. Thus, a transected proximal nerve stump may be lengthened by axonal regeneration during gradual stretching, and the stimulus of mechanical stretching may induce the growth of regenerating axons. ß

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A study of degeneration and regeneration in the divided rat sciatic nerve based on electron microscopy

Cited by 153 publications

References 0 publications

Glial Cell Line-Derived Neurotrophic Factor Alters Axon Schwann Cell Units and Promotes Myelination in Unmyelinated Nerve Fibers

Glial Cell Line-Derived Neurotrophic Factor Alters Axon Schwann Cell Units and Promotes Myelination in Unmyelinated Nerve Fibers

Development of a Multiple-Lumen Nerve Cuff Utilizing Growth Stimulant Patterns for Controlled Regeneration

Gradual stretching of the proximal nerve stump induces the growth of regenerating sprouts in rats

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