Gradual stretching of the proximal nerve stump induces the growth of regenerating sprouts in rats

Saijilafu,; Nishiura, Yasumasa; Hara, Yuki; Yoshii, Yukie; Ochiai, Naoyuki

doi:10.1002/jor.20587

Cited by 9 publications

(4 citation statements)

References 28 publications

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“…In proximal nerve stumps, regenerating sprouts will grow out from the first and second nodes of Ranvier proximal to the lesion 13, 32. The result of our previous study showed that it is possible to lengthen these regenerating axons by mechanically stretching the transected proximal nerve stump and that axonal lengthening occurs at the same rate as nerve stump lengthening 26. Furthermore, the nerve regeneration after the repair of segmental peripheral nerve defects with proximal nerve stump lengthening was as good as that induced by autogeneous nerve grafting 25.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous gradual lengthening of both proximal and distal nerve stumps for repair of peripheral nerve defect in rats

et al. 2008

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We investigated nerve regeneration following the repair of a segmental nerve defect induced by direct end-to-end neurorrhaphy after simultaneous gradual lengthening of both proximal and distal nerve stumps in rats. A 15-mm-long nerve segment was resected from the sciatic nerve of each rat. The proximal and distal nerve stumps, respectively, were directly lengthened at a rate of 1 mm/day using a custom-made external nerve-lengthening device. After being lengthened for 14 days, both nerve stumps were refreshed, and direct end-to-end neurorrhaphy was performed. For a control, 15-mm nerve grafting was performed immediately after nerve resection. Nerve regeneration was evaluated by motor nerve conduction velocity, muscle contraction force, and histological studies at 6, 8, and 14 weeks after initial nerve resection in both groups. As a result, at 8 and 14 weeks, the motor nerve conduction velocity was significantly higher in the nerve-lengthening group than in the autografting group. In addition, at 14 weeks, the tetanic force and wet weight of the gastrocnemius muscle were significantly higher in the nerve-lengthening group than in the autografting group. Histologically, the mean axonal diameter of myelinated nerve fibers and the total number of myelinated nerve fibers were also significantly higher in the nerve-lengthening group than in the autografting group for each evaluation period. It appears that the simultaneous gradual lengthening of both proximal and distal nerve stumps might have potential application in the repair of peripheral nerve defects.

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Section: Discussionmentioning

confidence: 99%

Simultaneous gradual lengthening of both proximal and distal nerve stumps for repair of peripheral nerve defect in rats

et al. 2008

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“…Tissue encapsulation of the cuff, a common effect [2], [35-45], may serve to stabilize the cuff over the life of the implant and lead to growth and partial recovery of damage done to the nerve [43]. Nerve growth has been shown to be positively influenced by strains induced under natural conditions [59], [60]; as a result, it is possible that a sustained increase in nerve tension during chronic cuff implantation may promote mechanically induced growth pathways within the axon leading to ameliorative growth [61]. …”

Section: Discussionmentioning

confidence: 99%

Biomechanical and functional variation in rat sciatic nerve following cuff electrode implantation

Restaino

Abliz

Wachrathit

et al. 2014

J NeuroEngineering Rehabil

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BackgroundNerve cuff electrodes are commonly and successfully used for stimulating peripheral nerves. On the other hand, they occasionally induce functional and morphological changes following chronic implantation, for reasons not always clear. We hypothesize that restriction of nerve mobility due to cuff implantation may alter nerve conduction.MethodsWe quantified acute changes in nerve-muscle electrophysiology, using electromyography, and nerve kinematics in anesthetized Sprague Dawley rat sciatic nerves during controlled hindlimb joint movement. We compared electrophysiological and biomechanical response in uncuffed nerves and those secured within a cuff electrode using analysis of variance (ANOVA) and regression analysis.ResultsTethering resulting from cuff implantation resulted in altered nerve strain and a complex biomechanical environment during joint movement. Coincident with biomechanical changes, electromyography revealed significantly increased variability in the response of conduction latency and amplitude in cuffed, but not free, nerves following joint movement.ConclusionOur findings emphasize the importance of the mechanical interface between peripheral nerves and their devices on neurophysiological performance. This work has implications for nerve device design, implantation, and prediction of long-term efficacy.

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“…In recent years, nerve lengthening has been studied as a new way of repairing a segmental nerve defect. We have investigated direct lengthening of a proximal nerve stump in rats [1,2], but we have not studied the direct lengthening of a distal nerve stump. Before this study, we could elongate a distal nerve stump up to the point at which both stumps could be repaired without tension, and the lengthening distal nerve stump was elongated not only at the epineurium but also at the band of Bü ngner in the endoneurium.…”

Section: Introductionmentioning

confidence: 99%

Repair of peripheral nerve defect by direct gradual lengthening of the distal nerve stump in rats: Effect on nerve regeneration

Yamada

Nishiura

Saijilafu

et al. 2009

Scandinavian Journal of Plastic and Reconstructive Surgery and

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We investigated the use of direct lengthening of the distal stump of a peripheral nerve to compare the results of nerve regeneration using the direct lengthening method with simple end-to-end suture and autografting in rats. A segment 10 mm long was resected from the rat sciatic nerve (n=18 in each group). The distal nerve stump was fixed to a ring and pulled at a rate of 1 mm/day for 20 days using an original external nerve distraction device. The results showed that the degree of nerve regeneration in the lengthened group was superior to that of the grafted group electrophysiologically and histologically, but there were no significant differences between the lengthened and end-to-end suture groups. We conclude that direct lengthening of the distal stump of a peripheral nerve can promote nerve regeneration similar to that observed in a Wallerian degenerated nerve. We think that this technique may be used for the treatment of peripheral nerve injuries.

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Gradual stretching of the proximal nerve stump induces the growth of regenerating sprouts in rats

Cited by 9 publications

References 28 publications

Simultaneous gradual lengthening of both proximal and distal nerve stumps for repair of peripheral nerve defect in rats

Simultaneous gradual lengthening of both proximal and distal nerve stumps for repair of peripheral nerve defect in rats

Biomechanical and functional variation in rat sciatic nerve following cuff electrode implantation

Repair of peripheral nerve defect by direct gradual lengthening of the distal nerve stump in rats: Effect on nerve regeneration

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