A study of calcium absorption using an automated fluorimetric assay procedure

Rushton, M. L.; Sammons, H. G.; Robinson, Brian H.

doi:10.1016/0009-8981(71)90286-5

Cited by 10 publications

(2 citation statements)

References 8 publications

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“…Lewin et al (1969), Moser and Gerarde (1969), and Bandrowski and Benson (1972) have used this order of alkali concentration in manual methods where calcium is determined using calcein. Rushton et al (1971) have automated the method of Lewin, and include EDTA in the calcein reagent to 'back off' fluorescence below 8 mg/IOO ml calcium, in …”

Section: Discussionmentioning

confidence: 99%

Continuous Flow Techniques for the Determination of Serum Calcium and the Simultaneous Determination of Calcium and Inorganic Phosphate in Serum and Urine

Brydon

1974

Ann Clin Biochem

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The fluorometric method of Fingerhut et al. (1969) for the determination of serum calcium has been modified to approximately treble the sensitivity. Using only 120 μl of sample a method has been developed for the simultaneous determination of calcium and inorganic phosphate in serum and urine, the phosphate being measured by complexing phosphomolybdate with methyl green, a method developed by Van Belle (1970). Only a single dialysis stage is required, and reagents and sample can be delivered using an AutoAnalyzer Pump I. The techniques correlate well with other methods. Accuracy and precision data are presented.

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Section: Discussionmentioning

confidence: 99%

Continuous Flow Techniques for the Determination of Serum Calcium and the Simultaneous Determination of Calcium and Inorganic Phosphate in Serum and Urine

Brydon

1974

Ann Clin Biochem

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“…The calcium results obtained from those albumin solutions diluted in saline were used to correct for the calcium content of the albumin preparation. The results obtained showed that over the protein range studied (5 g/l-l00 g/I) the calcium measured did not vary from the expected value of 2.50 mmol/I, Phosphate and magnesium interference was studied by the method of Rushton et al (1971). Phosphate was found to interfere at levels greater than 20 mmol/l; a phosphate concentration of 30 mmol/l decreased the serum calcium by 0.10 mmol/l (at a level of 2.50 mmolJl).…”

Section: Working Blank Reagentmentioning

confidence: 94%

A Study of Factors Influencing Continuous Flow Kinetics: The Use of Serum Calcium Estimation as a Model

Spencer¹

1976

Ann Clin Biochem

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Previous studies of continuous flow kinetics have described an "AutoAnalyzer" peak in terms of two time constants, lag constant "a" and exponential constant "b". Further investigations described here define some of the factors which affect these constants and hence peak shape and analytical performance. This communication outlines the application of this information to the development of an automated method for the determination of serum calcium, having a carry-over of less than 1% and an analytical rate greater than 100/hour. The instaneous colour reaction, simple hydraulics, removal of the dialysis step, and the use of an integral debubbling flow cell have enabled small kinetic constants to be achieved. Over a period of three months' routine use between-batch co-efficient of variation (C.V.) was 1.4%.

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Pathophysiology of Calcium Absorptive Disorders

Birge

Avioli

1986

Physiology of Membrane Disorders

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A study of calcium absorption using an automated fluorimetric assay procedure

Cited by 10 publications

References 8 publications

Continuous Flow Techniques for the Determination of Serum Calcium and the Simultaneous Determination of Calcium and Inorganic Phosphate in Serum and Urine

Continuous Flow Techniques for the Determination of Serum Calcium and the Simultaneous Determination of Calcium and Inorganic Phosphate in Serum and Urine

A Study of Factors Influencing Continuous Flow Kinetics: The Use of Serum Calcium Estimation as a Model

Pathophysiology of Calcium Absorptive Disorders

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