A study of bone marrow iron storage in hematological disorder

Pujara, Krupal M; Bhalara, Rohit V; Dhruva, Gauravi

doi:10.4103/2278-344x.143052

Cited by 10 publications

(20 citation statements)

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“…[5] IDA was the most common finding in our study with a iron store of 0 to 1+. This finding was similar to the finding in Pujara et alMA was second most common finding recorded in our study with a normal iron store(2+ to 3+).…”

Section: Discussionmentioning

confidence: 46%

“…[7] Pujara et al also found absent to low normal marrow iron content in the cases of aplastic anaemia. [5] This dissimilarity may be due to lesser number of cases of AA recorded in our study. Increased iron store in AA may be due to associated autoimmune disorder which causes AA.…”

Section: Discussionmentioning

confidence: 64%

“…While Pujara et al found increased store , Dharwadkar et al found normal (1+to 3+) iron store in bone marrow fragments. [5,6] This dissimilarity with Pujara et al may be due to the grading followed by them who considered 1+ to 2+ iron as normal store while grades above that was recorded as increased store.…”

Section: Discussionmentioning

confidence: 90%

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Importance of Perls’ stain as a routine test in anaemia in adults

Deka

Saikia

Kakati

et al. 2017

APALM

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Background: Anaemia is one of the frequent finding in patients of all age. Proper diagnosis is of utmost important to avoid fatal morbidity in untreated chronic cases. Aims: This study is meant to observe the role of Perls' stain in routine practice in etiological evaluation of anaemia in adults. Methods:Prospective cross sectional study from June 2007 to July 2010. 128 cases of anaemia, alone or as a part of bicytopenia and pancytopenia, in 20-80 years of age, who were recommended by clinicians for bone marrow aspiration analysis after initial routine haematological investigation, were collected as samples. Perls' stain was done on bone marrow smears to estimate marrow iron store in fragments and ring sideroblasts in all the smears.Result: Anaemia was most common haematological presentation(49%), mainly in males (62%) and majority in 20-30 years of age (31.3%). Iron deficiency anaemia was most common cause of anaemia(59.6%) with 0 to1+ iron store. Megaloblastic anaemia, aplastic anaemia, thalassemia and other haemoglobinnopathies, anaemia of chronic disease(AOCD) , myelodysplastic syndrome(MDS) and secondary sideroblastic anaemia(SA) were other causes of anaemia found. 3.2% cases had ring sideroblasts of which 0.78% was MDS and 2.3% was secondary SA. Conclusion:Perls' stain is a cheap and relatively simple test that can be used as a routine test in all bone marrow aspirates to provide provisional diagnosis of some relatively infrequent causes of anaemias which otherwise could be missed when only non invasive methods are relied upon for assessing body iron content.

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Section: Discussionmentioning

confidence: 46%

Section: Discussionmentioning

confidence: 64%

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Importance of Perls’ stain as a routine test in anaemia in adults

Deka

Saikia

Kakati

et al. 2017

APALM

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“…Nevertheless, iron profile is preferred now-a-day because it is a non-invasive test. 4 Megaloblastic anemia presents as macrocytic normochromic anemia. Megaloblasts are found in bone marrow aspirate smear which display nucleocytoplasmic asynchrony.…”

Section: Introductionmentioning

confidence: 99%

Bone marrow aspiration cytology in the diagnosis of hematologic and non-hematologic diseases in a multi-specialty hospital in Nepal

et al. 2017

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Background: Peripheral blood smear examination and other routine laboratory assays are not always sufficient to diagnose various diseases which affect the blood and bone marrow. A bone marrow aspirate examination is essential in most of the cases.Methods: This work was a hospital-based cross sectional analytical observational study carried out in the department of pathology at the Chitwan Medical College, Teaching Hospital, Nepal, over a period of 3 years from January, 2013 to December 2015. Bone marrow was aspirated from posterior superior iliac crest under local anesthesia; sternum was the alternative site in obese patients. Univariate analysis was performed for each variable using frequency distribution and means with the help of Statistical Package for Social Sciences 20.0 (SPSS 20.0) software.Results: One hundred and fifty-nine patients were included in the study. Eight cases of 'dry tap' were excluded, for whom bone marrow biopsy was advised. Anemia was the largest group followed by malignancy, infection and miscellaneous diseases. Among anemia, megaloblastic anemia was the most common, followed by aplastic anemia, dyserythropoietic anemia and myelopthisic anemia. The category 'malignancy' consisted of leukemia, multiple myeloma and myelodysplastic syndrome. Regarding individual diseases, megaloblastic anemia was the most common haematological disorder followed by immune thrombocytopenic purpura, leukemia and aplastic anemia.Conclusions: Bone marrow aspiration cytology is a mildly invasive technique which can diagnose many haematological and non-hematologic diseases that can be confirmed by more advanced investigations, if needed: serological, biochemical or molecular. However, bone marrow sample cannot be obtained (dry tap) in a proportion of cases. In such cases, a bone marrow biopsy needs to be performed.

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“…then removed and rinsed with distilled water. Counterstaining with saffranin for 30 s. then allowed to dry and examined [14] .…”

Section: Patient and Methodsmentioning

confidence: 99%