A study of aerobic bacterial pathogens associated with vaginitis in reproductive age group women (15-45 years) and their sensitivity pattern

Sangeetha, K.; Golia, Saroj; Vasudha, C L

doi:10.18203/2320-6012.ijrms20150615

Cited by 13 publications

(2 citation statements)

References 12 publications

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“…Similar risk factors predispose to aerobic vaginitis (AV), which is a type of vaginal dysbiosis often overlooked and that can be misdiagnosed as BV. AV is often characterized by more extreme inflammatory changes than BV and by the presence of mainly aerobic enteric commensals or pathobionts, including Group B Streptococcus (S. agalactiae), Enterococcus faecalis, Escherichia coli, and Staphylococcus aureus [9], living as non-harming symbionts under normal circumstances [10]. These pathobionts occur at lower levels than BV-anaerobes but with a higher pathogenic potential.…”

Section: Introductionmentioning

confidence: 99%

Vaginal Dysbiosis and Partial Bacterial Vaginosis: The Interpretation of the “Grey Zones” of Clinical Practice

et al. 2021

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Bacterial vaginosis (BV) affects one-third of reproductive age women, increasing the risk of acquiring sexually transmitted infections (STIs) and posing a risk for reproductive health. The current diagnosis with Gram stain (Nugent Score) identifies a transitional stage named partial BV or intermediate microbiota, raising the problem of how to clinically handle it. We retrospectively analyzed cervicovaginal swabs from 985 immunocompetent non-pregnant symptomaticspp. women (vaginal discharge, burning, itching) by Nugent score and qPCR for BV, aerobic or fungal vaginitis, and STIs (Mycoplasmas spp., Chlamydia t., Trichomonas v., and Neisseria g.). Nugent scores 0–3 and 7–10 were confirmed in 99.3% and 89.7% cases, respectively, by qPCR. Among Nugent scores 4–6 (partial BV), qPCR identified 46.1% of BV cases, with 37.3% of cases negative for BV, and only 16.7% of partial BV. Gram staining and qPCR were discordant (p value = 0.0001) mainly in the partial BV. Among the qPCR BV cases, the presence of aerobic vaginitis and STIs was identified, with a significant association (p < 0.0001) between the STIs and partial BV/overt BV. qPCR is more informative and accurate, and its use as an alternative or in combination with Gram staining could help clinicians in having an overview of the complex vaginal microbiota and in the interpretation of partial BV that can correspond to vaginitis and/or STIs.

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Section: Introductionmentioning

confidence: 99%

Vaginal Dysbiosis and Partial Bacterial Vaginosis: The Interpretation of the “Grey Zones” of Clinical Practice

et al. 2021

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“…Recently, enterococci have turned out to be one of the most imperative nosocomial pathogens, causing mortality up to 61% (Lopes et al, 2005). E. faecalis is considered the etiological agent of almost 80 percent of human infections and is incriminated in the pathogenesis of AV at a rate of 32.26% followed by E. coli (25.8%) (Sangeetha et al, 2015;Daood et al, 2020). The symptoms of AV include thinned vaginal mucosa, increased vaginal inflammation, and invariably an abundant yellowish foul-odor vaginal discharge (Donders et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

In vivo studies could not confirm in vitro prophylactic synergism between Moringa essential oil and Lactobacillus reuteri (MT180537)

et al. 2024

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Aerobic vaginitis (AV) is a recently defined vaginal recurring infection, which is treated with antibiotics. However, excessive and prolonged use of antibiotics disrupts healthy vaginal microflora and leads to the emergence of antibiotic resistance among pathogens. This situation has directed researchers to explore alternative antimicrobials. The current study describes in vitro and in vivo antimicrobial efficacy and pharmaceutical interactions between plant essential oils (EOs) and five lactic acid bacteria (LABs), isolated from the healthy vagina, against E. faecalis, one of the major etiological agents of AV. In vitro experiments confirm good antimicrobial activity of both plant EOs and cell free supernatant (CFS) from LABs. Based on high antimicrobial efficacy, Moringa essential oil (MO) was selected to determine its nature of interaction with CFS of five LAB strains. Synergism was recorded between MO and CFS of L. reuteri (MT180537). To validate in vitro findings, prophylactic responses of individual and synergistic application of MO and L. reuteri (MT180537) were evaluated in an E. faecalis (MW051601) induced AV murine model. The prophylactic efficacy was evidenced by a reduction in intensity of clinical symptoms, E. faecalis (MW051601) count per vaginal tissue along with a reduction in AV associated changes in histological markers of infection in animals receiving Moringa essential oil and L. reuteri (MT180537) alone or in combination. However, significant synergism between Moringa essential oil and L. reuteri (MT180537) could not be observed. Our data confirms the importance of in vivo experiments in deducing pharmacological interactions.

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Evaluation of inhibitory and probiotic properties of lactic acid bacteria isolated from vaginal microflora

Shazadi

Arshad

2022

Folia Microbiol

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A study of aerobic bacterial pathogens associated with vaginitis in reproductive age group women (15-45 years) and their sensitivity pattern

Cited by 13 publications

References 12 publications

Vaginal Dysbiosis and Partial Bacterial Vaginosis: The Interpretation of the “Grey Zones” of Clinical Practice

Vaginal Dysbiosis and Partial Bacterial Vaginosis: The Interpretation of the “Grey Zones” of Clinical Practice

In vivo studies could not confirm in vitro prophylactic synergism between Moringa essential oil and Lactobacillus reuteri (MT180537)

Evaluation of inhibitory and probiotic properties of lactic acid bacteria isolated from vaginal microflora

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