A study of acid phosphatase of the mosquito Aedes aegypti L.

Lambremont, Edward N.

doi:10.1016/0022-1910(59)90034-4

Cited by 13 publications

(8 citation statements)

References 8 publications

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“…The QlO value (2·1) between 20 and 30°C is also normal and close to the values reported by Lambremont (1959), Ashrafi and Fisk (1961), and Gilbert and Huddleston (1965).…”

Section: Discussionsupporting

confidence: 89%

“…Optimum Huddleston and Gilbert (1961) The relationship between activity and substrate concentration was similar to that reported by Lambremont (1959), Ashrafi and Fisk (1961), and Gilbert and Huddleston (1965). The Km value (3·0xlO-4 M) compares well with that of acid phosphatase from stable fly (9· 7 X 10-4 M) and giant milkweed bug (1· 74 X lO-3M) reported by Ashrafi and Fisk but not with that of enzyme from A. aegypti (8·57 X 10-2M).…”

Section: Discussionsupporting

confidence: 83%

“…Fitzgerald (1949), Rockstein and Herron (1951), Rockstein (1956), Lambremont (1959), Ashrafi and Fisk (1962), and Gilbert and Huddleston (1965) have also reported magnesium ion as an activator of acid phosphatases. The optimum concentration of magnesium ion for enzyme activation was found to be 0·025M.…”

Section: Discussionmentioning

confidence: 96%

“…The optimum concentration of magnesium ion for enzyme activation was found to be 0·025M. Fitzgerald (1949) reported an optimum magnesium ion concentration for acid phosphatase activation of 0·05M, Lambremont (1959) reported 0'08M, and Rockstein (1956) reported 0·16-O' 20M. Thus the acid phosphatase of the desert locust required the least concentration for maximum activation.…”

Section: Discussionmentioning

confidence: 97%

“…During the last two decades work on biochemical characterization of phosphatases in insects has been done by Drilhon and Busnel (1945), Fitzgerald (1949), Rockstein and Herron (1951), Rockstein and Levine (1951), Denuce (1952), Rockstein (1956), Barker and Alexander (1958), Lambremont (1959), Fisk (1961, 1962), Hodgson (1963), Sridhara and Bhat (1963), Huddleston (1965), McCaman, Smith, andCock (1965), Raychaudri and Butz (1965), Hirano and Gilbert (1967), and Naqvi, Ashrafi, and Qadri (1967).…”

Section: Introduotionmentioning

confidence: 99%

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Acid Phosphatase Activity in the Digestive System of the Desert Locust Schistocerca Gregaria (Forskal)

Ashrafi¹,

Qadri²

1968

Aust. Jnl. Of Bio. Sci.

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SummaryAcid phosphatase activity was determined biochemically in a homogenate of the digestive tract of the desert locust, S. gregaria. p-Nitrophenol was used as colorimetric standard and disodium p.nitrophenyl phosphate as substrate.The enzyme was found to have a linear relationship between concentration and activity, maximum activity at pH 4· 4, a Michaelis constant of 3·0 X 10-4M, and a temperature coefficient (QlO) of 2 ·10 between 20 and 30°C. Zero-order kinetics were maintained for 35 min at 40°C. Magnesium, calcium, and potassium ions and magnesium and phenylmercuric acetates slightly activated the enzyme, while ferrous ion, sodium fluoride, disodium hydrogen phosphate, and disodium hydrogen arsenate inhibited the enzyme. The magnesium ion concentration required for maximum activation was found to be O· 025M. INTRODUOTIONPhosphomonoesterases or phosphoric monoester hydrolases (3.l.3, Thompson 1962) are widely present in animal and plant tissues. During the last two decades work on biochemical characterization of phosphatases in insects has been done by Drilhon and Busnel (1945), Fitzgerald (1949), Rockstein and Herron (1951), Rockstein and Levine (1951), Denuce (1952), Rockstein (1956), Barker and Alexander (1958), Lambremont (1959), Fisk (1961, 1962), Hodgson (1963), Sridhara and Bhat (1963), Huddleston (1965), McCaman, Smith, andCock (1965), Raychaudri and Butz (1965), Hirano and Gilbert (1967), and Naqvi, Ashrafi, and Qadri (1967).In general these authors have studied the phosphomonoesterases in homogenates of holometabolous insects, whereas the present work deals with the biochemical characterization of acid phosphatase in the alimentary canal of a hemimetabolous insect, the desert locust, Schistocerca gregaria (Forskal). II. MATERIALS AND METHODS Enzyme SourceAdult locusts (28 days old) which had been fed on 6% glucose solution for 1 day prior to the experiment were selected as the source of enzymes. Locusts were dissected in cold doubledistilled demineralized water and the alimentary canal was transferred to a test tube containing 5 ml chilled double-distilled demineralized water. It was ground for exactly 3 min in a TeflonPyrex tissue grinder. The homogenate was filtered through a 2-mm layer of glass fibre in a

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“…The QlO value (2·1) between 20 and 30°C is also normal and close to the values reported by Lambremont (1959), Ashrafi and Fisk (1961), and Gilbert and Huddleston (1965).…”

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

Section: Introduotionmentioning

confidence: 99%

See 3 more Smart Citations

Acid Phosphatase Activity in the Digestive System of the Desert Locust Schistocerca Gregaria (Forskal)

Ashrafi¹,

Qadri²

1968

Aust. Jnl. Of Bio. Sci.

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Biochemical basis of alphamethrin resistance in different life stages ofAnopheles stephensistrains of Bangalore, India

Hariprasad

Shetty

2016

Pest Management Science

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BACKGROUND: Anopheles stephensi is an important urban malaria vector in the Indian subcontinent. Extensive application of insecticides evokes microevolution, which results in resistance that can be traced back to their genotypes. In this study, resistant and susceptible strains of An. stephensi for alphamethrin were selected by selective inbreeding for 27 and ten generations respectively. The biochemical basis of resistance in all the life stages was investigated. Quantitative assays were performed for proteins (total and soluble), esterases ( , and acetylcholine) and phosphatases (acid and alkaline) by spectrophotometry, and qualitative assays for the enzymes by native polyacrylamide gel electrophoresis. RESULTS:The enzyme quantities significantly varied in all life stages of the resistant strain as compared with the susceptible ones. Qualitative studies showed seven isoforms for -and -esterases, three each for acetylcholinesterase and alkaline phosphatase and two for acid phosphatase. Exclusive bands were found in the resistant strain, such as -Est 1 and -Est 1 in eggs and larvae, -Est 3 in adult males, -Est 2 in adult females and AlkP 1, AlkP 2 and AlkP 3 in adult females, larvae and adult males respectively. CONCLUSION: Variations in the quantity and specific enzyme isoforms play a key role in the development of alphamethrin resistance in An. stephensi.

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Nachweis und Charakterisierung von Phosphorsäureester spaltenden Enzymen in der Hämolymphe von Lepidopterenraupen

Jarczyk

Tietz

1962

Naturwissenschaften

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A study of acid phosphatase of the mosquito Aedes aegypti L.

Cited by 13 publications

References 8 publications

Acid Phosphatase Activity in the Digestive System of the Desert Locust Schistocerca Gregaria (Forskal)

Acid Phosphatase Activity in the Digestive System of the Desert Locust Schistocerca Gregaria (Forskal)

Biochemical basis of alphamethrin resistance in different life stages ofAnopheles stephensistrains of Bangalore, India

Nachweis und Charakterisierung von Phosphorsäureester spaltenden Enzymen in der Hämolymphe von Lepidopterenraupen

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