A study in vitro on differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in mice

Zhang, Wenbi; Cheng, Mingjun; Huang, Yu‐Ting; Jiang, Wei; Cong, Qing; Zheng, Yufang; Xu, Congjian

doi:10.1016/j.ejogrb.2011.10.012

Cited by 33 publications

(35 citation statements)

References 29 publications

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“…Therefore, our data strongly suggested that this disadvantage of BMSCs administration in endometriosis model was largely due to E2 secretion. A few previous studies demonstrated the contribution of BMSCs in development of endometriosis3536. However, the explanations were mainly around the ability of BMSCs to differentiate into epithelial cells35 and repopulation of endometrium36.…”

Section: Discussionmentioning

confidence: 99%

Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application

Peng²,

Zeng³

et al. 2015

Sci Rep

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Mesenchymal stem cells are therapeutically applicable and involved in the development of some types of diseases including estrogen (E2)-related ones. Little is known about E2 secretion by mesenchymal stem cells and its potential influence on their therapeutical applications. Our in vitro experiments showed that BMSCs cultured from C57BL/6J mice secreted E2 in a time-dependent manner. In vivo study identified a significantly increased E2 level in serum after a single administration of BMSCs, and a sustained elevation of E2 level upon a repetitive administration. Morris water maze test in the ovariectomised (OVX) mouse model revealed BMSCs transplantation ameliorated OVX-induced memory deficits by secreted E2. On the contrary, in endometriosis model, BMSCs transplantation aggravated endometriotic lesions because of E2 secretion. Mechanistically, the aromatase cytochrome P450 appeared to be critical for the biosynthesis and exerted effects of estrogen secretion by BMSCs. Our findings suggested that BMSCs transplantation is on the one hand an attractive option for the therapeutic treatment of diseases associated with E2 deficits in part through E2 secretion, on the other hand a detrimental factor for the E2-exasperated diseases largely via E2 production. It is important and necessary to monitor serum E2 level before and after the initiation of BMSCs therapy.

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Section: Discussionmentioning

confidence: 99%

Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application

Peng²,

Zeng³

et al. 2015

Sci Rep

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“…After bone marrow transplantation in women and mice, genetic chimerism of endometrial cells was observed (Figueira et al 2011). Moreover, mouse BMSC under the influence of estradiol (E 2 ) and the environment of endometrial stromal cells differentiated into endometrial epithelial cells (Zhang et al 2012). Therefore, bone marrow may also constitute the source of cells involved in endometrial regeneration (Figueira et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

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The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8-10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus.

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“…Glandular nests may arise de-novo deep in the myometrium from undifferentiated stem cells in response to exogenous stimuli. Mesenchymal stem cells may also migrate to uterine tissues from the bone marrow with blood or through the lymphatic vessels, and once there they may differentiate into endometrial epithelial cells under E2 influence (Gargett 2006, Zhang et al 2012. Our recent studies on bovine uterine cells also suggest the involvement of stem cells in the pathogenesis of adenomyosis.…”

Section: Metaplasia Processmentioning

confidence: 86%

Is adenomyosis a problem in reproduction and fertility?

Korzekwa

Łupicka

Socha

et al. 2014

Polish Journal of Veterinary Sciences

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Adenomyosis is defined as the presence of glandular foci beside the endometrium of uterus: in the myometrium and/or perimetrium depending on the progress of the disorder. So far, adenomyosis has been diagnosed in women and rodents, and studies conducted on cows have been rare. In this review we: (1) summarize the knowledge regarding adenomyosis, (2) compare the symptoms and aetiopathology between women and cows, (3) describe angiogenic uterine processes related to adenomyosis development and (4) outline the influence of adenomyosis on proper fertility processes in cattle (conception and fertility rates).

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A study in vitro on differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in mice

Cited by 33 publications

References 29 publications

Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application

Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Is adenomyosis a problem in reproduction and fertility?

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