Is adenomyosis a problem in reproduction and fertility?

Korzekwa, Anna; Łupicka, M.; Socha, Barbara; Mannelli, Chiara; Skarzynski, Dariusz J.

doi:10.2478/pjvs-2014-0028

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…According to this hypothesis, glandular nests may arise de novo within the myometrial layer from undifferentiated stem cells under specific conditions, in particular under the influence of oestradiol (E 2 ) [ 7 , 8 ]. Whatever the mechanism underlying formation of glandular foci in the myometrium, hormonal and immunological abnormalities certainly play a role during adenomyosis development [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Expression of pluripotency markers in the bovine uterus with adenomyosis

Łupicka

Socha

Szczepańska

et al. 2015

Reprod Biol Endocrinol

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BackgroundAdenomyosis is a proliferative uterine dysfunction with unknown aetiology. One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue. Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.FindingsImmunolocalisation revealed protein expression of NANOG, OCT4 and SOX2 in both normal and adenomyotic uteri. mRNA expression for NANOG and OCT4 was increased in tissues obtained from uteri with adenomyosis compared to controls, but at the protein level there were no significant differences. mRNA expression for all three pluripotency markers was higher in myometrial cells isolated from uteri with adenomyotic lesions than in those isolated from normal uteri. The protein level of NANOG and SOX2 was decreased in stromal cells from adenomyotic tissues, whereas the level of OCT4 and SOX2 was increased in myometrial cells obtained from dysfunctional uteri.ConclusionsThe results indicate significant changes in expression of pluripotency markers in adenomyotic compared to normal uteri, which suggest the involvement of uterine stem cells in adenomyosis.

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Section: Introductionmentioning

confidence: 99%

Expression of pluripotency markers in the bovine uterus with adenomyosis

Łupicka

Socha

Szczepańska

et al. 2015

Reprod Biol Endocrinol

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“…As a special form of endometriosis, the typical clinical features of adenomyosis include menorrhagia, dysmenorrhea, metrorrhagia and infertility (1)(2)(3). The medications most commonly used to treatments for adenomyosis are hormonal, including the levonorgestrel intrauterine system and gonatropin-releasing hormone agonists.…”

Section: Introductionmentioning

confidence: 99%

Berberine inhibits the LPS‑induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway

et al. 2017

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Abstract.A previous study by our group has demonstrated that lipopolysaccharide (LPS) induces adenomyosis through stimulating inflammatory cell proliferation and invasive growth of stromal cells via Toll-like receptor 4 (TLR4) signaling. The present study aimed to investigate the effects of berberine (BBR) on LPS-induced ectopic endometrial stromal cells (EESCs) isolated from patients with adenomyosis. The viability of EESCs treated with LPS or LPS plus BBR was detected by a cell counting kit-8 assay, and the cell cycle distribution and apoptosis were evaluated by flow cytometry. The effect of BBR on the expression of key molecules of inflammatory proliferation and invasive growth of LPS-induced EESCs was also evaluated. BBR significantly inhibited the LPS-induced proliferation of EESCs in a dose-and time-dependent manner. BBR induced cell cycle arrest in G0/G1 phase and enhanced apoptosis of LPS-induced EESCs. Furthermore, BBR inhibited the expression of interleukin (IL)-6, IL-8, transforming growth factor-β, epithelial growth factor, vascular endothelial growth factor and matrix metalloproteinase 2 in LPS-induced EESCs. To the best of our knowledge, the present study was the first to demonstrate that BBR has a protective effect on ameliorating the LPS-induced progression of adenomyosis. This result may provide a novel therapeutic strategy for the clinical treatment of the disease.

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“…These days of the estrous cycle were selected for experiments because this study is continuation of our concerning adenomyosis (Korzekwa et al 2013(Korzekwa et al , 2014(Korzekwa et al , Łupicka et al 2015 and adenomyosis has been observed during the luteal stage of the estrous cycle in cattle (Moreira et al 2007). Uterine tissues were obtained at the Meat Processing Plant "Warmia" (Biskupiec, Poland) and transported on ice to the laboratory within 40 min.…”

Section: Materials Collectionmentioning

confidence: 99%

“…Adenomyosis is a uterine dysfunction characterized by the presence of endometrial glands with stromal elements in the myometrium (Azziz 1989, Campo et al 2012) but the etiology of this disorder is still unclear (Ferenczy 1998, Korzekwa et al 2014. Our previous studies showed that in cows the freCorrespondence to: A. Korzekwa, e-mail: a.korzekwa@pan.olsztyn.pl, tel.…”

Section: Introductionmentioning

confidence: 99%

Estradiol Reduces Connexin43 Gap Junctions in the Uterus during Adenomyosis in Cows

Korzekwa¹,

Łupicka²,

Socha³

et al. 2016

Polish Journal of Veterinary Sciences

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Adenomyosis is defined as the presence of glandular foci external to the endometrium of the uterus, either in the myometrium or/and perimetrium, depending on the progress of this dysfunction. To date, we showed that steroids secretion and prolactin expression and proliferative processes are disturbed during uterine adenomyosis in cows. During endometriosis in eutopic endometrium in women, gap junctions are down regulated. The transmembrane gap junction protein, connexin (Cx43) is necessary for endometrial morphological, biochemical and angiogenic functions. The aim of this study is recognition of adenomyosis etiology by determination of the role of Cx43 in this process. Immunolocalization and comparison of Cx43 mRNA and protein expression in healthy (N=9) and adenomyotic uterine tissue (N=9), and Cx43 mRNA expression (real time PCR) in uterine stromal -myometrium co-culture under 24-hour stimulation with 17-beta estradiol (10 -7 M) isolated from healthy (N=5) and adenomyotic (N=5) cows were determined. Cx43 was localized in healthy and adenomyotic uteri. mRNA and protein expression was down-regulated in uterine tissue in adenomyotic compared with healthy cows (p<0.05). Estradiol stimulated Cx43 mRNA expression in myometrial cell culture and co-culture of stromal and myometrial cells in adenomyotic compared with healthy cows (p<0.05). In summary, down-regulation of Cx43 expression in the junction zone might play an important role in pathogenesis of adenomyosis. Estradiol modulates gap junctions during adenomyosis.

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Is adenomyosis a problem in reproduction and fertility?

Cited by 9 publications

References 40 publications

Expression of pluripotency markers in the bovine uterus with adenomyosis

Expression of pluripotency markers in the bovine uterus with adenomyosis

Berberine inhibits the LPS‑induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway

Estradiol Reduces Connexin43 Gap Junctions in the Uterus during Adenomyosis in Cows

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