A study focused on macrophages modulation induced by the Polymeric Electrospun Matrices (EL-Ms) for application in tissue regeneration: In vitro proof of concept

Pisani, Silvia; Dorati, Rossella; Benazzo, Marco; Conti, Bice; Prina-Mello, Adriele

doi:10.1016/j.ijpharm.2021.120712

Cited by 9 publications

(9 citation statements)

References 44 publications

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“…The results showed an initial pro-inflammatory response (after 24 h) characterized by release of Tumor Necrosis Factor (TNF)-alfa, interleukin (IL)-6 and IL-8 cytokines which decreases and is substituted by preferential anti-inflammatory response rising after 72 h, with IL-10 release and elongation of macrophages typical of M2 cell polarization. These results confirm PLA-PCL electrospun matrices can be eligible as support to enhance tissue regeneration promoting an anti-inflammatory response [233].…”

Section: Examples Of Pla and Derivatives Scaffolds For Tissue Regenerationsupporting

confidence: 62%

“…An interesting in vitro study was conducted by Pisani et al [233] evaluating the reaction of monocytes and macrophages cells after contact with a biomaterial. The results give a preliminary scenario regarding immune system reaction after contact with electrospun matrices made from PLA-PCL 70:30 ratio, which can be useful since regenerative process has to consider the role of immune system after surgical implantation of a polymer-based matrix.…”

Section: Examples Of Pla and Derivatives Scaffolds For Tissue Regenerationmentioning

confidence: 99%

“…The encouraging positive results achieved in the mentioned experimental works [231][232][233] led to start an in vivo experimentation on animal model involving pig esophagus transplant that is currently ongoing (data not published yet).…”

Section: Examples Of Pla and Derivatives Scaffolds For Tissue Regenerationmentioning

confidence: 99%

“…Such one product obtained through coating a decellularized and deproteinized bone matrix of bovine origin with a special grade of PLA, has been studied [223] and could be eligible as a medical device. Instead, PLA-PCL electrospun matrices proved capable to induce modifications in macrophages (M0) by modulating in vitro M1 and/or M2 macrophages polarization, thus promoting an anti-inflammatory response [233]. Based on the type of cell loading and on the mechanism of action such matrices could then be eligible to become an advanced therapy product.…”

Section: Regulatory Aspectsmentioning

confidence: 99%

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Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

et al. 2021

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Tissue repair and regeneration is an interdisciplinary field focusing on developing bioactive substitutes aimed at restoring pristine functions of damaged, diseased tissues. Biomaterials, intended as those materials compatible with living tissues after in vivo administration, play a pivotal role in this area and they have been successfully studied and developed for several years. Namely, the researches focus on improving bio-inert biomaterials that well integrate in living tissues with no or minimal tissue response, or bioactive materials that influence biological response, stimulating new tissue re-growth. This review aims to gather and introduce, in the context of Italian scientific community, cutting-edge advancements in biomaterial science applied to tissue repair and regeneration. After introducing tissue repair and regeneration, the review focuses on biodegradable and biocompatible biomaterials such as collagen, polysaccharides, silk proteins, polyesters and their derivatives, characterized by the most promising outputs in biomedical science. Attention is pointed out also to those biomaterials exerting peculiar activities, e.g., antibacterial. The regulatory frame applied to pre-clinical and early clinical studies is also outlined by distinguishing between Advanced Therapy Medicinal Products and Medical Devices.

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Section: Examples Of Pla and Derivatives Scaffolds For Tissue Regenerationsupporting

confidence: 62%

Section: Examples Of Pla and Derivatives Scaffolds For Tissue Regenerationmentioning

confidence: 99%

Section: Examples Of Pla and Derivatives Scaffolds For Tissue Regenerationmentioning

confidence: 99%

Section: Regulatory Aspectsmentioning

confidence: 99%

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Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

et al. 2021

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“…Machado et al [ 47 ] recently demonstrated that the immune response is modulated in Caco-2 cells in the presence of pomegranate oil (PO), with a significant reduction in the secretion of IL-6 and IL-8 in unstimulated cells, as well as a significant reduction in all cytokines (TNF-α, IL-6 and Il-8) in stimulated cells. Therefore, it is possible to understand that the molecules TNF-α, IL-6 and IL-8 participate in inflammatory responses [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System

et al. 2023

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In recent years, lycopene has been highlighted due to its antioxidant and anti-inflammatory properties, associated with a beneficial effect on human health. The aim of this study was to advance the studies of antioxidant and anti-inflammatory mechanisms on human keratinocytes cells (HaCaT) of a self-emulsifying drug delivery system (SEDDS) loaded with lycopene purified from red guava (nanoLPG). The characteristics of nanoLPG were a hydrodynamic diameter of 205 nm, a polydispersity index of 0.21 and a zeta potential of −20.57, providing physical stability for the nanosystem. NanoLPG demonstrated antioxidant capacity, as shown using the ORAC methodology, and prevented DNA degradation (DNA agarose). Proinflammatory activity was evaluated by quantifying the cytokines TNF-α, IL-6 and IL-8, with only IL-8 showing a significant increase (p < 0.0001). NanoLPG showed greater inhibition of the tyrosinase and elastase enzymes, involved in the skin aging process, compared to purified lycopene (LPG). In vitro treatment for 24 h with 5.0 µg/mL of nanoLPG did not affect the viability of HaCaT cells. The ultrastructure of HaCaT cells demonstrated the maintenance of morphology. This contrasts with endoplasmic reticulum stresses and autophagic vacuoles when treated with LPG after stimulation or not with LPS. Therefore, the use of lycopene in a nanoemulsion may be beneficial in strategies and products associated with skin health.

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Electrospun fiber‐based immune engineering in regenerative medicine

Xu,

Saiding,

Zhou

et al. 2024

Smart Medicine

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Immune engineering, a burgeoning field within regenerative medicine, involves a spectrum of strategies to optimize the intricate interplay between tissue regenerative biomaterials and the host tissue. These strategies are applied across different types of biomaterials and various disease models, which encompasses finely modulating the immune response at the levels of immune cells and factors, aiming to mitigate adverse effects like fibrosis and persistent inflammation that may arise at the injury site and consequently promote tissue regeneration. With the continuous progress in electrospinning technology, the immunoregulatory capabilities of electrospun fibers have gained substantial attention over the years. Electrospun fibers, with their extracellular matrix‐like characteristics, high surface‐area‐to‐volume ratio, and reliable pharmaceutical compound capacity, have emerged as key players among tissue engineering materials. This review specifically focuses on the role of electrospun fiber‐based immune engineering, emphasizing their unique design strategies. Notably, electrospinning actively engages in immune engineering by modulating immune responses through four essential strategies: (i) surface modification, (ii) drug loading, (iii) physicochemical parameters, and (iv) biological grafting. This review presents a comprehensive overview of the intricate mechanisms of the immune system in injured tissues while unveiling the key strategies adopted by electrospun fibers to orchestrate immune regulation. Furthermore, the review explores the current developmental trends and limitations concerning the immunoregulatory function of electrospun fibers, aiming to drive the advancements in electrospun fiber‐based immune engineering to its full potential.

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A study focused on macrophages modulation induced by the Polymeric Electrospun Matrices (EL-Ms) for application in tissue regeneration: In vitro proof of concept

Cited by 9 publications

References 44 publications

Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System

Electrospun fiber‐based immune engineering in regenerative medicine

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