Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System

Abstract

In recent years, lycopene has been highlighted due to its antioxidant and anti-inflammatory properties, associated with a beneficial effect on human health. The aim of this study was to advance the studies of antioxidant and anti-inflammatory mechanisms on human keratinocytes cells (HaCaT) of a self-emulsifying drug delivery system (SEDDS) loaded with lycopene purified from red guava (nanoLPG). The characteristics of nanoLPG were a hydrodynamic diameter of 205 nm, a polydispersity index of 0.21 and a zeta potential of −20.57, providing physical stability for the nanosystem. NanoLPG demonstrated antioxidant capacity, as shown using the ORAC methodology, and prevented DNA degradation (DNA agarose). Proinflammatory activity was evaluated by quantifying the cytokines TNF-α, IL-6 and IL-8, with only IL-8 showing a significant increase (p < 0.0001). NanoLPG showed greater inhibition of the tyrosinase and elastase enzymes, involved in the skin aging process, compared to purified lycopene (LPG). In vitro treatment for 24 h with 5.0 µg/mL of nanoLPG did not affect the viability of HaCaT cells. The ultrastructure of HaCaT cells demonstrated the maintenance of morphology. This contrasts with endoplasmic reticulum stresses and autophagic vacuoles when treated with LPG after stimulation or not with LPS. Therefore, the use of lycopene in a nanoemulsion may be beneficial in strategies and products associated with skin health.

Self-emulsifying drug delivery systems and the application in drugs and cosmetics

“…Các biến số khác như loại, nồng độ chất diện hoạt, nhiệt độ trộn và tốc độ khuấy khi pha hữu cơ được thêm vào pha nước cũng có tác động đến đường kính trung bình của các giọt [68]. Nano nhũ tương chứa lycopen bào chế được bằng phương pháp tự nhũ hoá cho kích thước giọt là 205 nm, PDI 0,21 đã chứng minh khả năng chống oxy hóa, chống viêm, chống lão hoá da, có tiềm năng trong các sản phẩm chăm sóc da [69].…”