A Study and New Description of<i>Corynebacterium Vaginale (Haemophilus Vaginalis)</i>

Dunkelberg, W. E.; Skaggs, Romulus; Kellogg, Douglas S.

doi:10.1093/ajcp/53.3.370

Cited by 41 publications

(31 citation statements)

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“…PSD medium was prepared as described (8). Mixed organisms were originally from anaerobic cultures on blood agar as described in Methods.…”

Section: Resultsmentioning

confidence: 99%

“…Chocolate agar plates were examined after 48 h for growth of H. vaginalis, which was identified as described by Pheifer et al (1). Pure subcultures of five separate isolates of H. vaginalis from chocolate agar plates were inoculated with a wire loop into sterile tubes that contained 5 ml of peptonestarch-dextrose (PSD) medium (8). Inoculated PSD medium and uninoculated medium controls were incubated anaerobically at 35°C in a Gas Pak jar.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Amine content of vaginal fluid from untreated and treated patients with nonspecific vaginitis.

Chen¹,

Forsyth²,

Buchanan³

et al. 1979

J. Clin. Invest.

189

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A B S T R A C T We examined the vaginal washings from patients with nonspecific vaginitis (NSV) to seek biochemical markers and possible explanations for the signs and symptoms of this syndrome. Seven amines were identified including methylamine, isobutylamine, putrescine, cadaverine, histamine, tyramine, and phenethylamine. These amines may contribute to the symptoms of NSV and may contribute to the elevated pH of the vaginal discharge. They may also be partly responsible for the "fishy" odor that is characteristic of vaginal discharges from these patients. Among the seven amines, putrescine and cadaverine were the most abundant and were present in all vaginal discharges from each of ten patients before treatment. These amines are produced in vitro during growth of mixed vaginal bacteria in chemically defined medium, presumably by decarboxylation of the corresponding amino acids. We hypothesize that anaerobic vaginal organisms, previously shown to be quantitatively increased in NSV, are responsible for the amine production, because metronidazole inhibited the production ofamines by vaginal bacteria in vitro, and Haemophilus vaginalis did not produce amines. H. vaginalis did release high concentrations of pyruvic acid and of amino acids during growth in peptone-starch-dextrose medium, whereas, other vaginal flora consumed both pyruvic acid and amino acids in the same medium during growth. These findings suggest that a symbiotic relationship may exist between H. vaginalis and other vaginal flora in patients with NSV.

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“…PSD medium was prepared as described (8). Mixed organisms were originally from anaerobic cultures on blood agar as described in Methods.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Amine content of vaginal fluid from untreated and treated patients with nonspecific vaginitis.

Chen¹,

Forsyth²,

Buchanan³

et al. 1979

J. Clin. Invest.

189

View full text Add to dashboard Cite

show abstract

“…In addition, growth was tested on the peptone-starch-dextrose (PSD) medium of Dunkelberg et al (14) and on campylobacter blood-free selective agar base (2), which contains charcoal, casein, and 0.1% deoxycholate. Growth anaerobically at 37°C was tested in a jar that was evacuated and filled three times with a gas mixture containing 10% CO,, 10% H,, and 80% N, and also in an anaerobic chamber.…”

Section: Analysis Of Respiratory Quinonesmentioning

confidence: 99%

Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov., Respectively

Goodwin

Armstrong

Chilvers

et al. 1989

International Journal of Systematic Bacteriology

511

205

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“…The G. vaginalis strains used in this study (see Table 1) are designated by the numbers used in the University of Queensland Department of Microbiology (UQM). Strain UQM1258 was obtained from the National Collection of Type Cultures as NCTC 10287; all other strains were freshly isolated from vaginal specimens, G. vaginalis isolates were identified by the cultural and physiological characteristics recommended by Dunkelberg et al (6).…”

Section: Methodsmentioning

confidence: 99%