A Student Team in a University of Michigan Biomedical Engineering Design Course Constructs a Microfluidic Bioreactor for Studies of Zebrafish Development

Shen, Yu Chi; Li, David; Al-Shoaibi, Ali; Bersano-Begey, Tom; Chen, Hao; Ali, Shahid; Flak, Betsy; Perrin, Catherine; Winslow, Max B.; Shah, Harsh; Ramamurthy, Poornapriya; Schmedlen, Rachael H.; Takayama, Shuichi; Barald, Kate F.

doi:10.1089/zeb.2008.0572

Cited by 23 publications

(15 citation statements)

References 46 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, there has been a trend for zebrafish-related study turning to lab-on-a-chip, such as high-efficient embryonic microinjection, 25 high-throughput embryonic droplets generation, 26 and digital transportation of droplet encapsulated embryo, 27 ranging from dynamic manipulation to static stimulation by electroporation 28 and microfluids. 29 However, the microscale droplet is ill suited for long-time culture of embryos, particularly in small-size segments, 26 and the autocrine or paracrine may affect their normal development. Although the on-chip research for zebrafish ͑embryos͒ have been successfully performed, their significant application in drug discovery and toxic assessment are still limited, and the corresponding technical challenges also exist.…”

mentioning

confidence: 99%

An integrated microfluidic array system for evaluating toxicity and teratogenicity of drugs on embryonic zebrafish developmental dynamics

et al. 2011

View full text Add to dashboard Cite

Seeking potential toxic and side effects for clinically available drugs is considerably beneficial in pharmaceutical safety evaluation. In this article, the authors developed an integrated microfluidic array system for phenotype-based evaluation of toxic and teratogenic potentials of clinical drugs by using zebrafish ͑Danio rerio͒ embryos as organism models. The microfluidic chip consists of a concentration gradient generator from upstream and an array of open embryonic culture structures by offering continuous stimulation in gradients and providing guiding, cultivation and exposure to the embryos, respectively. The open culture reservoirs are amenable to long-term embryonic culturing. Gradient test substances were delivered in a continuous or a developmental stage-specific manner, to induce embryos to generate dynamic developmental toxicity and teratogenicity. Developmental toxicity of doxorubicin on zebrafish eggs were quantitatively assessed via heart rate, and teratological effects were characterized by pericardial impairment, tail fin, notochord, and SV-BA distance /body length. By scoring the teratogenic severity, we precisely evaluated the time-and dose-dependent damage on the chemical-exposed embryos. The simple and easily operated method presented herein demonstrates that zebrafish embryo-based pharmaceutic assessment could be performed using microfluidic systems and holds a great potential in high-throughput screening for new compounds at single animal resolution.

show abstract

mentioning

confidence: 99%

An integrated microfluidic array system for evaluating toxicity and teratogenicity of drugs on embryonic zebrafish developmental dynamics

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Multiple flow-through systems have been developed to allow observation of zebrafish development over time with the ability to introduce compounds on demand 15,16,17 . More complex devices have been designed to array embryos while still within their chorion, which provides a simple spherical geometry that can be easily manipulated with relatively high throughput in these systems 18,19 .…”

Section: Discussionmentioning

confidence: 99%

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Ellett

Irimia

2017

JoVE

View full text Add to dashboard Cite

Zebrafish have emerged as a powerful model of various human diseases and a useful tool for an increasing range of experimental studies, spanning fundamental developmental biology through to large-scale genetic and chemical screens. However, many experiments, especially those related to infection and xenograft models, rely on microinjection and imaging of embryos and larvae, which are laborious techniques that require skill and expertise. To improve the precision and throughput of current microinjection techniques, we developed a series of microstructured devices to orient and stabilize zebrafish embryos at 2 days post fertilization (dpf) in ventral, dorsal, or lateral orientation prior to the procedure. To aid in the imaging of embryos, we also designed a simple device with channels that orient 4 zebrafish laterally in parallel against a glass cover slip. Together, the tools that we present here demonstrate the effectiveness of photolithographic approaches to generate useful devices for the optimization of zebrafish techniques.

show abstract

“…In 2009, Shen et al presented a microfluidic chip for zebrafish embryo immobilization, whereby only the inferior of the embryo was exposed to the test compounds, and the embryo viability was maintained . The chip was made of two layers of PDMS: the top layer had a funnel‐shaped aperture to immobilize the embryo; and the bottom layer contained a microchannel to deliver fluidic samples . The chip was submerged in a Petri dish containing fish medium, and a gravity‐driven pump was used to deliver test compounds via the microchannel .…”

Section: First Steps To Miniaturization: Microfluidic Chip‐based Embrmentioning

confidence: 99%

Fishing on chips: Up‐and‐coming technological advances in analysis of zebrafish and Xenopus embryos

et al. 2014

View full text Add to dashboard Cite

Biotests performed on small vertebrate model organisms provide significant investigative advantages as compared with bioassays that employ cell lines, isolated primary cells, or tissue samples. The main advantage offered by whole-organism approaches is that the effects under study occur in the context of intact physiological milieu, with all its intercellular and multisystem interactions. The gap between the high-throughput cell-based in vitro assays and low-throughput, disproportionally expensive and ethically controversial mammal in vivo tests can be closed by small model organisms such as zebrafish or Xenopus. The optical transparency of their tissues, the ease of genetic manipulation and straightforward husbandry, explain the growing popularity of these model organisms. Nevertheless, despite the potential for miniaturization, automation and subsequent increase in throughput of experimental setups, the manipulation, dispensing and analysis of living fish and frog embryos remain labor-intensive. Recently, a new generation of miniaturized chip-based devices have been developed for zebrafish and Xenopus embryo on-chip culture and experimentation. In this work, we review the critical developments in the field of Lab-on-a-Chip devices designed to alleviate the limits of traditional platforms for studies on zebrafish and clawed frog embryo and larvae. V C 2014 International Society for Advancement of Cytometry

show abstract

A Student Team in a University of Michigan Biomedical Engineering Design Course Constructs a Microfluidic Bioreactor for Studies of Zebrafish Development

Cited by 23 publications

References 46 publications

An integrated microfluidic array system for evaluating toxicity and teratogenicity of drugs on embryonic zebrafish developmental dynamics

An integrated microfluidic array system for evaluating toxicity and teratogenicity of drugs on embryonic zebrafish developmental dynamics

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Fishing on chips: Up‐and‐coming technological advances in analysis of zebrafish and Xenopus embryos

Contact Info

Product

Resources

About