A structure–gelation ability study in a short peptide-based ‘Super Hydrogelator’ system

Wang, Huaimin; Yang, Cuihong; Tan, Ming; Wang, Ling; Kong, Deling; Yang, Zhimou

doi:10.1039/c0sm01405b

Cited by 82 publications

(55 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A sample of P2b passed the inversion test at a concentration as low as 0.006 wt-%, (Fig. S8) which is about an order of magnitude lower in concentration than many of the well-known synthetic superhydrogelators 23 .…”

mentioning

confidence: 99%

Responsive biomimetic networks from polyisocyanopeptide hydrogels

Kouwer

Koepf

Sage

et al. 2013

Nature

470

561

View full text Add to dashboard Cite

Responsive hydrogels applied in the biomedical area show great potential as synthetic extracellular matrix mimics and as host medium for cell growth. The hydrogels often lack the characteristic mechanical properties that are typically seen for natural gels. Here, we demonstrate the unique responsive and mechanical properties of hydrogels based on oligo(ethylene glycol) functionalized polyisocyanopeptides. These stiff helical polymers form gels upon warming at concentrations as low as 0.006 %-wt polymer, with materials properties almost identical to those of their intermediate filaments, a class of cytoskeletal proteins. Using a combination of macroscopic rheology and molecular force microscopy the hierarchical relationship between the macroscopic behaviour of theses peptide mimics has been correlated with the molecular parameters.

show abstract

mentioning

confidence: 99%

Responsive biomimetic networks from polyisocyanopeptide hydrogels

Kouwer

Koepf

Sage

et al. 2013

Nature

470

561

View full text Add to dashboard Cite

show abstract

“…21 Within our system, less efficient overlap of the Fmoc groups is observed in gelled samples, and similar emission trends have been reported in Fmoc-peptide families post-gelation. 20, 21, 45 In water, self-assembly leads to critical gelation density only after 3 weeks. During the initial stages of assembly, it is possible that the fluorenyl group transitions from an orientation with a very high degree of stacking to a much less ordered arrangement.…”

Section: Resultsmentioning

confidence: 99%

Charge and sequence effects on the self-assembly and subsequent hydrogelation of Fmoc-depsipeptides

2014

View full text Add to dashboard Cite

Herein we report on the self-assembly of a family of Fmoc-depsipeptides into nanofibers and hydrogels. We show that fiber formation occurs in depsipeptide structures in which the fluorenyl group is closely associated and that side-chain charge and sequence affect the extent of self-assembly and subsequent gelation. Using fluorescence emission spectroscopy and circular dichroism, we show that self-assembly can be monitored and is observed in these slow-gelling systems prior to hydrogel formation. We also demonstrate that the ionic strength of salt-containing solutions affects the time at which self-assembly results in gelation of the bulk solution. From transmission electron microscopy, we report that morphological changes progress over time and are observed as micelles transitioning to fibers prior to the onset of gelation. Gelled depsipeptides degraded at a slower rate than non-gelled samples in the presence of salt, while hydrolysis in water of both gels and solution samples was minimal even after 14 days. Our work shows that while incorporating ester functionality within a peptide backbone reduces the number of hydrogen bonding sites available for forming and stabilizing supramolecular assemblies, the substitution does not prohibit self-assembly and subsequent gelation.

show abstract

“…The value of G' was more than an order of magnitude greater than that of G'', indicating the formation of a true hydrogel. [1,43] Atomic force microscopy (AFM) was used to characterize the morphology of microstructures in the gel. As shown in Figure 3A, nanofibers with uniform size (25 nm) were observed and some of them were helical.…”

Section: Resultsmentioning

confidence: 99%

Orthogonal Enzymatic Reactions to Control Supramolecular Hydrogelations

Chen

Ren

et al. 2011

Chin. J. Chem.

Self Cite

View full text Add to dashboard Cite

Enzyme-responsive hydrogels have great potential in applications of controlled drug release, tissue engineering, etc. In this study, we reported on a supramolecular hydrogel that showed responses to two enzymes, phosphatase which was used to form the hydrogels and esterase which could trigger gel-sol phase transitions. The gelation process and visco-elasticity property of the resulting gel, morphology of the nanostructures in hydrogel, and peptide conformation in the self-assembled nanostructure were characterized by rheology, transmission electron microscope (TEM), and circular dichroism (CD), respectively. Potential application of the enzyme-responsive hydrogel in drug release was also demonstrated in this study. Though only one potential application of drug release was proved in this study, the responsive hydrogel system in this study might have potentials for the applications in fields of cell culture, controlled-drug release, etc.

show abstract

A structure–gelation ability study in a short peptide-based ‘Super Hydrogelator’ system

Cited by 82 publications

References 76 publications

Responsive biomimetic networks from polyisocyanopeptide hydrogels

Responsive biomimetic networks from polyisocyanopeptide hydrogels

Charge and sequence effects on the self-assembly and subsequent hydrogelation of Fmoc-depsipeptides

Orthogonal Enzymatic Reactions to Control Supramolecular Hydrogelations

Contact Info

Product

Resources

About