A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres

Liu, Xiao; Yang, Jifei; Li, Qingmei; Wang, Yinbiao; Wang, Yanhong; Li, Ge; Shi, Jianzhou; Ding, Peiyang; Guo, Junqing; Deng, Ruiguang; Zhang, Gaiping

doi:10.1007/s00604-020-04255-1

Cited by 23 publications

(17 citation statements)

References 29 publications

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“…Colloidal gold was prepared by trisodium citrate method (22). Briefly, 1 mL of 1% chloroauric acid was added to the erlenmeyer flask with 99 mL double distilled water which was stirring and heating, followed by the rapid addition of 1.6 mL of 1% trisodium citrate solution with rapid stirring.…”

Section: Preparation Of Colloidal Gold and Gold-labeled Monoclonal Anmentioning

confidence: 99%

Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein

Wang

Chen

et al. 2021

Front. Immunol.

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The outbreak and worldwide pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have a significant impact on global economy and human health. In order to reduce the disease spread, 16 monoclonal antibodies (McAbs) again SARS-CoV-2 were generated by immunized mice with the spike protein receptor binding domain (RBD), which was expressed in Chinese hamster ovary cell (CHO). A colloidal gold-based immunochromatographic strip was developed with two McAbs to detect SARS-CoV-2 spike protein, which can play a potential role in monitoring vaccine quality. The strip is highly specific, detecting only SARS-CoV-2 spike protein, and does not show any non-specific reactions with syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and other coronavirus and influenza viruses. The strip detected subunit vaccine in our laboratory with a detection limit of spike protein of 62.5 ng/mL. This strip provides an effective method in monitoring vaccine quality by detecting the antigen content of spike protein.

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Section: Preparation Of Colloidal Gold and Gold-labeled Monoclonal Anmentioning

confidence: 99%

Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein

Wang

Chen

et al. 2021

Front. Immunol.

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“…The lateral flow assay strip had a detection limit that was 50 times lower than conventional AuNPs. In another study, Liu et al coated AuNPs in polystyrene latex microspheres (PS) to increase the sensitivity for determining influenza virus H3 subtype [ 100 ]. Along with the sandwich format, the use of AuNPs-PS as a reporter for influenza virus detection can achieve 64 times higher sensitivity than that of 10 nm AuNPs.…”

Section: Enhancement Of Sensitivity and Specificitymentioning

confidence: 99%

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

Hsiao

Pham

et al. 2021

Biosensors

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The development of reliable and robust diagnostic tests is one of the most efficient methods to limit the spread of coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, most laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, and require highly trained professional operators. On the other hand, the lateral flow immunoassay (LFIA) is a simpler, cheaper device that can be operated by unskilled personnel easily. Unfortunately, the current technique has some limitations, mainly inaccuracy in detection. This review article aims to highlight recent advances in novel lateral flow technologies for detecting SARS-CoV-2 as well as innovative approaches to achieve highly sensitive and specific point-of-care testing. Lastly, we discuss future perspectives on how smartphones and Artificial Intelligence (AI) can be integrated to revolutionize disease detection as well as disease control and surveillance.

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“…Preparation of colloidal gold and gold-labeled antibodies Preparation of colloidal gold by trisodium citrate method [16]. Brie y, 1 mL of 1% chloroauric acid and 99 mL of double distilled water was added into the erlenmeyer ask, stiring and heating, followed by the rapid addition of 1.6 mL of 1% trisodium citrate solution with rapid stirring.…”

Section: Virus Neutralization Testmentioning

confidence: 99%

Development of an Immunochromatographic Strip for Rapid Detection of H7 Subtype Avian Influenza Viruses

Wang

et al. 2021

Preprint

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Background: H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. H7N9 AIV has a high mortality rate after infection in humans, and most human cases have close contacted with poultry in the live poultry market. Therefore, it is necessary to develop a rapid point-of-care testing (POCT) technique for H7N9 AIV detection.Methods: The H7N9 AIV was inactivated and purified, and was used as the antigen to immunize BALB/c. Twelve H7-HA specific monoclonal antibodies (McAbs) were produced through the hybridoma technique. The McAb 10A8 was conjugated with colloid gold as detecting antibody; McAb 9B6 was dispensed on the nitrocellulose membran as the capture test line and the Goat-anti mouse IgG antibody was dispensed as control line respectively. The immunochromatographic strip was prepared.Results: The analysis of ELISA and virus neutralization test showed that the obtained McAbs specifically recognized H7 HA. Based on the prepared strip, the detection of H7 AIV was achieved within 10 min. No cross-reaction occurred between H7 AIVs and other tested viruses. The detection limit of the strip for H7 was 2.4 log10EID50/0.1 mL for chicken swab samples.Conclusion: The McAbs were specific for H7 and the immunochromatographic strip developed in this study was convenient, rapid and reliable for the detection of H7 AIV. The strip could provide an effective method for the rapid and early detection of H7 AIV.

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A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres

Cited by 23 publications

References 29 publications

Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein

Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

Development of an Immunochromatographic Strip for Rapid Detection of H7 Subtype Avian Influenza Viruses

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