A Striking Organization of a Large Family of Human Neural Cadherin-like Cell Adhesion Genes

Wu, Qiang; Maniatis, Tom

doi:10.1016/s0092-8674(00)80789-8

Cited by 668 publications

(700 citation statements)

References 55 publications

Supporting

Mentioning

685

Contrasting

Unclassified

Order By: Relevance

“…In mammals, related molecules with 34-cadherin repeats have been described (4,6,8,9,21,22). In order to clarify their evolutionary relationship, we compared their predicted amino acid sequences.…”

Section: Structure Of the Drosophila Fat-like (Ftl)mentioning

confidence: 99%

The fat-like Gene of Drosophila Is the True Orthologue of Vertebrate Fat Cadherins and Is Involved in the Formation of Tubular Organs

Castillejo-López¹,

Arias²,

Baumgärtner³

2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

Fat cadherins constitute a subclass of the large cadherin family characterized by the presence of 34 cadherin motifs. To date, three mammalian Fat cadherins have been described; however, only limited information is known about the function of these molecules. In this paper, we describe the second fat cadherin in Drosophila, fat-like (ftl). We show that ftl is the true orthologue of vertebrate fat-like genes, whereas the previously characterized tumor suppressor cadherin, fat, is more distantly related as compared with ftl. Ftl is a large molecule of 4705 amino acids. It is expressed apically in luminal tissues such as trachea, salivary glands, proventriculus, and hindgut. Silencing of ftl results in the collapse of tracheal epithelia giving rise to breaks, deletions, and sac-like structures. Other tubular organs such as proventriculus, salivary glands, and hindgut are also malformed or missing. These data suggest that Ftl is required for morphogenesis and maintenance of tubular structures of ectodermal origin and underline its similarity in function to a reported lethal mouse knock-out of fat1 where glomerular epithelial processes collapse. Based on our results, we propose a model where Ftl acts as a spacer to keep tubular epithelia apart rather than the previously described adhesive properties of the cadherin superfamily.

show abstract

Section: Structure Of the Drosophila Fat-like (Ftl)mentioning

confidence: 99%

The fat-like Gene of Drosophila Is the True Orthologue of Vertebrate Fat Cadherins and Is Involved in the Formation of Tubular Organs

Castillejo-López¹,

Arias²,

Baumgärtner³

2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The variable exons of the Pcdh-␣ and Pcdh-␥ clusters are classified into several subgroups, Pcdh-␣, Pcdh-␥A, Pcdh-␥B, and C-type (4,10). The Pcdh-␣ gene cluster contains the variable exons Pcdh-␣1 to Pcdh-␣12, and in addition, ␣7/8 exists in wild mouse strains and two C-type variable exons, C1 and C2 (10,14,15).…”

mentioning

confidence: 99%

Allelic Gene Regulation of Pcdh-α and Pcdh-γ Clusters Involving Both Monoallelic and Biallelic Expression in Single Purkinje Cells

Kaneko

Kato

Kawamura

et al. 2006

Journal of Biological Chemistry

179

217

View full text Add to dashboard Cite

The molecular basis for providing the identity and diversity of single neurons is a key for realizing the brain system. Diverse protocadherin isoforms encoded by the Pcdh-␣ and Pcdh-␥ gene clusters are expressed in all of the vertebrates studied. For the Pcdh-␣ isoforms, differential expression patterns have been found in single Purkinje cells by unusual monoallelic and combinatorial types of gene regulation. Here we investigated total allelic gene regulation in the Pcdh-␣ and -␥ clusters, including the C-type variable exons (C1 to C5) and the Pcdh-␥A and -␥B variable exons in single Purkinje cells. Using split single-cell reverse transcription-PCR analysis, almost all of the Purkinje cells at postnatal day 21 biallelically expressed all the C-type isoforms, whereas the Pcdh-␣ isoforms showed both monoallelic and combinatorial expression. The Pcdh-␥A and -␥B isoforms also showed differential regulation in each cell with both monoallelic and combinatorial gene regulation. These data indicated that different types of allelic gene regulation (monoallelic versus biallelic) occurred in the Pcdh-␣ and -␥ clusters, although they were spliced into the same constant exons. It has been reported that each C-type Pcdh-␣ or -␥ transcript has a different expression pattern during brain development, suggesting that the different C-type variable exons may code temporal diversity, although the Pcdh-␣, -␥A, and -␥B isoforms were differential and combinatorial gene regulation within a single cell. Thus, the multiple gene regulations in the Pcdh-␣ and -␥ clusters had a potential mechanism for increasing the diversity of individual neurons in the brain.

show abstract

“…Moreover, as no obvious logic correlates cell body position of the projection neuron, birth time and the location of the glomerular projection, simple molecular gradient/counter-gradient models as used for axon guidance in the retinotectal system 16,17 are unlikely to suf®ce. Instead, we propose that dendrites of projection neurons use a combination of recognition molecules speci®c to each eventual glomerulus; mechanisms that generate a complex repertoire of cell-surface molecules have recently been described 21,22 . Furthermore, our study uncovers an elegant mechanism for specifying different projection neurons: the precisely ordered generation by a single neuroblast of a large number of distinct neurons.…”

mentioning

confidence: 99%

Target neuron prespecification in the olfactory map of Drosophila

Jefferis

Marin

Stocker³

et al. 2001

Nature

389

437

View full text Add to dashboard Cite

A Striking Organization of a Large Family of Human Neural Cadherin-like Cell Adhesion Genes

Cited by 668 publications

References 55 publications

The fat-like Gene of Drosophila Is the True Orthologue of Vertebrate Fat Cadherins and Is Involved in the Formation of Tubular Organs

The fat-like Gene of Drosophila Is the True Orthologue of Vertebrate Fat Cadherins and Is Involved in the Formation of Tubular Organs

Allelic Gene Regulation of Pcdh-α and Pcdh-γ Clusters Involving Both Monoallelic and Biallelic Expression in Single Purkinje Cells

Target neuron prespecification in the olfactory map of Drosophila

Contact Info

Product

Resources

About