A Strategy for the Sequential Patterning of Proteins:  Catalytically Active Multiprotein Nanofabrication

Jang, Chang‐Hyun; Stevens, Benjamin D.; Phillips, Ryan; Calter, Michael A.

doi:10.1021/nl0259636

Cited by 34 publications

(32 citation statements)

References 7 publications

(13 reference statements)

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“…Commercial gold-coated glass slides (BIAcore, SIA kit) were pretreated and coated with a selfassembled monolayer (SAM) of alkanethiol derivatives, as described. 37 The alkane thiol had a vicinal diol on its other end, similar to what has been described earlier 38 ; its synthesis will be published elsewhere. Before use, the vicinal diols on the SAM were converted into aldehydes by incubation in buffer (100 mM sodium acetate, pH 5.5 adjusted with HCl) containing 10 mM NaIO 4 for 30 min.…”

Section: Covalent Immobilization Of Fibrinogen Onto Biosensor Surfacesmentioning

confidence: 75%

Kinetics of the interaction of desAABB–fibrin monomer with immobilized fibrinogen

et al. 2006

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The soluble and stable fibrin monomer-fibrinogen complex (SF) is well known to be present in the circulating blood of healthy individuals and of patients with thrombotic diseases. However, its physiological role is not yet fully understood. To deepen our knowledge about this complex, a method for the quantitative analysis of interaction between soluble fibrin monomers and surface-immobilized fibrinogen has been established by means of resonant mirror (IAsys) and surface plasmon resonance (BIAcore) biosensors. The protocols have been optimized and validated by choosing appropriate immobilization procedures with regeneration steps and suitable fibrin concentrations. The highly specific binding of fibrin monomers to immobilized fibrin(ogen), or vice versa, was characterized by an affinity constant of approximately 10(-8)M, which accords better with the direct dissociation of fibrin triads (KD approximately 10(-8) -10(-9) M) (J. R. Shainoff and B. N. Dardik, Annals of the New York Academy of Science, 1983, Vol. 27, pp. 254-268) than with earlier estimations of the KD for the fibrin-fibrinogen complex (KD approximately 10(-6) M) (J. L. Usero, C. Izquierdo, F. J. Burguillo, M. G. Roig, A. del Arco, and M. A. Herraez, International Journal of Biochemistry, 1981, Vol. 13, pp. 1191-1196).

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Section: Covalent Immobilization Of Fibrinogen Onto Biosensor Surfacesmentioning

confidence: 75%

Kinetics of the interaction of desAABB–fibrin monomer with immobilized fibrinogen

et al. 2006

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“…(a) nanografting [308][309][310][311][312] (b) (direct write) dip pen nanolithography. [313][314][315][316][317][318][319][320] Near field electrospinning, a recently introduced technique, 321 can also be used for polymeric NW fabrication and integration with microstructures.…”

Section: High Resolution Lithography and Serial Deposition Techniquesmentioning

confidence: 99%

Integration of one-dimensional nanostructures with microsystems: an overview

Alaca

2009

International Materials Reviews

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The current state of solutions provided for the issue of integration between micro-and nanoscales is reviewed for the specific case of nanowires and nanotubes. Such structures serve as field emitters, transistors or laser sources, digits for manipulation and handling, as sensing elements or as agents for the modification of surface properties such as the adhesive strength. However, it is noteworthy that the majority of reported device work remains confined to component level prototype development without the prospect of full scale system integration due to the lack of batch compatible fabrication and processing techniques. On the one hand, nanostructures made by self-assembly do not possess a high level of control on their orientation and numbers, and hence, their interfacing and integration with a microsystem pose difficulties. On the other hand, top-down approaches such as manipulation, serial deposition and high resolution lithographic techniques do not satisfy the needs of large scale fabrication due to their expensive and/or nonparallel working principles. These techniques along with hybrid approaches taking advantage of the structural control of self-assembly and geometric control of high resolution lithography will be discussed and major applications will be highlighted to shed light on the capabilities and limitations associated with each process.

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“…Using nTP, we are able to transfer the metal pattern from a stamp with the relief feature, which defines the geometry of the pattern, onto both conformal (thin poly(dimethylsiloxane) film supported on plastic) and rigid (e.g. Such approaches will be combined with other methods, such as micromachining [383,384], orthogonal self-assembly of electroactive monolayers [57,[385][386][387], enzymatic nanolithography [388][389][390], and nanofabrication using a nanopipet addressing [391]. They describe that using nTP, a wide range of patterns can be printed in parallel with nanometer length scale resolution over large areas.…”

Section: Microcontact Printing By Use Of Organosulfur Inksmentioning

confidence: 99%