A strategy for enzyme immobilization on layer-by-layer dendrimer–gold nanoparticle electrocatalytic membrane incorporating redox mediator

Crespilho, Frank N.; Ghica, Mariana Emilia; Florescu, Monica; Nart, Francisco Carlos; Oliveira, Osvaldo N.; Brett, Christopher M.A.

doi:10.1016/j.elecom.2006.07.032

Cited by 175 publications

(115 citation statements)

References 10 publications

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“…43 Numerous materials in LbL films are used for biosensing, including carbon nanotubes (CNTs), 44 graphene sheets, 45 metal nanoparticles, 46 and biomolecules. 47 Of particular importance in this regard is the control of molecular architecture, as exemplified in the biosensor schematically shown in Figure 3. The main aim in this specially designed architecture was to provide a friendly environment for adsorption of glucose oxidase (GOx) in order to detect glucose at a low potential and with high sensitivity.…”

Section: A Generic Architecture For Biosensorsmentioning

confidence: 99%

“…The enzyme glucose oxidase (GOx) was immobilized in a friendly environment obtained with a solution containing BSA and glutaraldehyde. Modified with permission from ref 47. Copyright 2006 Elsevier.…”

Section: A Generic Architecture For Biosensorsmentioning

confidence: 99%

“…185,186 In other instances, it is better to keep the enzyme only on the topmost layer because the main reactions occur on the surface. 47,187 Also worth mentioning is a nanoscale protein chip prepared with an etched polystyrene (PS) template, immobilized as LB films, used for an immunoassay exploring SERS spectra. 188 …”

Section: Graphenementioning

confidence: 99%

See 2 more Smart Citations

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Oliveira

Iost

Siqueira

et al. 2014

ACS Appl. Mater. Interfaces

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150

137

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Clinical diagnosis has always been dependent on the efficient immobilization of biomolecules in solid matrices with preserved activity, but significant developments have taken place in recent years with the increasing control of molecular architecture in organized films. Of particular importance is the synergy achieved with distinct materials such as nanoparticles, antibodies, enzymes, and other nanostructures, forming structures organized on the nanoscale. In this review, emphasis will be placed on nanomaterials for biosensing based on molecular recognition, where the recognition element may be an enzyme, DNA, RNA, catalytic antibody, aptamer, and labeled biomolecule. All of these elements may be assembled in nanostructured films, whose layer-by-layer nature is essential for combining different properties in the same device. Sensing can be done with a number of optical, electrical, and electrochemical methods, which may also rely on nanostructures for enhanced performance, as is the case of reporting nanoparticles in bioelectronics devices. The successful design of such devices requires investigation of interface properties of functionalized surfaces, for which a variety of experimental and theoretical methods have been used. Because diagnosis involves the acquisition of large amounts of data, statistical and computational methods are now in widespread use, and one may envisage an integrated expert system where information from different sources may be mined to generate the diagnostics.

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Section: A Generic Architecture For Biosensorsmentioning

confidence: 99%

Section: A Generic Architecture For Biosensorsmentioning

confidence: 99%

See 1 more Smart Citation

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Oliveira

Iost

Siqueira

et al. 2014

ACS Appl. Mater. Interfaces

Self Cite

150

137

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“…A new method for enzymatic immobilisation was recently demonstrated by us, which uses a combination of LbL self-assembly, redox mediator electrodeposition and cross-linking [7]. It was shown how one can benefit from combining the biocatalytic properties of the enzyme with the conductivity and electrocatalytic properties of gold nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

“…The present paper concerns several new developments with respect to the report in [7], focusing on optimising enzymatic devices and further characterising the biosensor architectures obtained with the LbL approach. Glucose oxidase (GOx) was the enzyme of choice because the catalytic properties of this enzyme are well known in the literature and because the determination of glucose concentration is important in clinical, biological and chemical samples, as well as in food processing and fermentation [14].…”

Section: Introductionmentioning

confidence: 99%

Enzyme immobilisation on electroactive nanostructured membranes (ENM): Optimised architectures for biosensing

Crespilho

Ghica

Gouveia‐Caridade

et al. 2008

Talanta

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a b s t r a c tElectroactive nanostructured membranes have been produced by the layer-by-layer (LbL) technique, and used to make electrochemical enzyme biosensors for glucose by modification with cobalt hexacyanoferrate redox mediator and immobilisation of glucose oxidase enzyme. Indium tin oxide (ITO) glass electrodes were modified with up to three bilayers of polyamidoamine (PAMAM) dendrimers containing gold nanoparticles and poly(vinylsulfonate) (PVS). The gold nanoparticles were covered with cobalt hexacyanoferrate that functioned as a redox mediator, allowing the modified electrode to be used to detect H 2 O 2 , the product of the oxidase enzymatic reaction, at 0.0 V vs. SCE. Enzyme was then immobilised by cross-linking with glutaraldehyde. Several parameters for optimisation of the glucose biosensor were investigated, including the number of deposited bilayers, the enzyme immobilisation protocol and the concentrations of immobilised enzyme and of the protein that was crosslinked with PAMAM. The latter was used to provide glucose oxidase with a friendly environment, in order to preserve its bioactivity. The optimised biosensor, with three bilayers, has high sensitivity and operational stability, with a detection limit of 6.1 M and an apparent Michaelis-Menten constant of 0.20 mM. It showed good selectivity against interferents and is suitable for glucose measurements in natural samples.

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Gold Nanoparticle‐Based Electrochemical Biosensors for Medical Applications

Anık

2012

Biomedical Materials and Diagnostic Devices

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A strategy for enzyme immobilization on layer-by-layer dendrimer–gold nanoparticle electrocatalytic membrane incorporating redox mediator

Cited by 175 publications

References 10 publications

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Enzyme immobilisation on electroactive nanostructured membranes (ENM): Optimised architectures for biosensing

Gold Nanoparticle‐Based Electrochemical Biosensors for Medical Applications

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