A Stem Cell Molecular Signature

Ivanovа, Natalia; Dimos, John; Schaniel, Christoph; Hackney, Jason A.; Moore, Kateri; Lemischka, Ihor R.

doi:10.1126/science.1073823

Cited by 1,387 publications

(1,154 citation statements)

References 21 publications

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“…Indeed, ES, HSC, and CNS stem cells share more than 200 genes, demonstrating the close relation between these cell types as well as their specific and common features at the transcriptional level. This gene profiling was done by using cDNA arrays for ES cells, gene enrichment for NSC and HSC by using subtractive hybridization (Ivanova et al, 2002;Ramalho-Santos et al, 2002).…”

Section: Can Anything Make Anything?mentioning

confidence: 99%

Mechanism of stem cells in the central nervous system

Johansson

2003

Journal Cellular Physiology

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Injury to the central nervous system (CNS) can result in severe functional impairment. The brain and spinal cord, which constitute the CNS, have been viewed for decades as having a very limited capacity for regeneration. However, over the last several years, the body of evidence supporting the concept of regeneration and continuous renewal of neurons in specific regions of the CNS has increased. This evidence has significantly altered our perception of the CNS and has offered new hope for possible cell therapy strategies to repair lost function. Transplantation of stem cells or the recruitment of endogenous stem cells to repair specific regions of the brain or spinal cord is the next exciting research challenge. However, our understanding of the existing stem cell pool in the adult CNS remains limited. This review will discuss the identification and characterization of CNS stem cells in the adult brain and spinal cord.

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Section: Can Anything Make Anything?mentioning

confidence: 99%

Mechanism of stem cells in the central nervous system

Johansson

2003

Journal Cellular Physiology

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“…The reported HSCs gene expression profiles include mouse fetal liver HSCs [41], human and mouse fetal liver HSCs and mouse BM HSCs [42], and mouse BM HSCs [43,44]. The global gene expression profiles of HSCs have identified growth factor receptors, cytokine receptors, hematopoietic growth factor receptors, G-protein coupled receptors (GPCR), morphogen receptors, hormone receptors, and putative genes encoding transmembrane proteins ( Table 2).…”

Section: Global Gene Expression Profiles Of Hscsmentioning

confidence: 99%

Local signals in stem cell-based bone marrow regeneration

Wang

Liu

2006

Cell Res

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The cellular basis of bone marrow (BM) tissue development and regeneration is mediated through hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs). Local interplays between hematopoietic cells and BM stromal cells (BMSCs) determine the reconstitution of hematopoiesis after myelosuppression. Here we review the BM local signals in control of BM regeneration after insults. Hematopoietic growth factors (HGFs) and cytokines produced by BMSCs are primary factors in regulation of BM hematopoiesis. Morphogens which are critical to early embryo development in multiple species have been added to the family of HSCs regulators, including families of Wnt proteins, Notch ligands, BMPs, and Hedgehogs. Global gene expression analysis of HSCs and BMSCs has begun to reveal signature groups of genes for both cell types. More importantly, analysis of global gene expression coupled with biochemical and biological studies of local signals during BM regeneration have strongly suggested that HGFs and cytokines may not be the primary local regulators for BM recovery, rather chemokines (SDF-1, FGF-4) and angiogenic growth factors (VEGF-A, Ang-1) play instructive roles in BM reconstitution after myelosuppression. A new direction of management of BM toxicity is emerging from the identification of BM regenerative regulators.

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“…Corresponding results were reported by [30], in which cat ES-like cells expressed the "stemness" transcription factors and the levels of expression of OCT-4, NANOG and C-MYC were reduced in cat ES-like colonies as compared to that seen in blastocysts. In other study, expression of transcription factors like Oct 4 and Nanog were used as molecular markers for ES cells in various species [16,17,[41][42][43]. Moreover, when undifferentiated primary colonies were analyzed for the expression of pluripotent transcription factors, Gómez et al [30] found that OCT-4 was down regulated regardless of the expression of NANOG.…”

Section: Discussionmentioning

confidence: 99%

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

Kumar

Anand

Singh

et al. 2011

J Assist Reprod Genet

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Purpose The aim of the present study is to compare the ability of homologous and heterologous embryonic fibroblast feeder layers to support isolation and proliferation of buffalo ES-like cells generated from hatched and expanded blastocysts produced by in vitro fertilization and characterization of derived cells through expression of pluripotent markers. Methods Embryonic stem cells were derived from hatched and expanded blastocysts through intact blastocyst culture and enzymatic method respectively and compared for proliferation rate on homologous (buffalo) and heterologous feeder layers (goat and sheep). Results A total of 69 hatched and 83 expanded blastocysts were used for isolation of inner cell masses which were seeded on buffalo, goat and sheep embryonic feeder layers. Following seeding, attachment rate, primary colony formation rate and survival to maximum number of passages were observed to be higher on homologous feeder layers.Conclusions Upon comparison of different feeder layer cells for derivation and maintenance of buffalo ES-like cells from hatched and expanded blastocysts, buffalo embryonic fibroblast cells were able to provide a better environment for maintaining pluripotency in culture conditions.

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A Stem Cell Molecular Signature

Cited by 1,387 publications

References 21 publications

Mechanism of stem cells in the central nervous system

Mechanism of stem cells in the central nervous system

Local signals in stem cell-based bone marrow regeneration

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

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