A statistically significant sex difference in the number of colony-forming cells from human peripheral blood

Hörner, Susanne; Pasternak, G; Hehlmann, R.

doi:10.1007/s002770050296

Cited by 11 publications

(6 citation statements)

References 20 publications

(16 reference statements)

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“…). As expected, MNCs from female donors contained fewer BFU‐E than those from males (Table ) but generated numbers of ERYs similar to those generated by MNCs from males both in HEMA ser and in HEMA def (Fig. ).…”

Section: Discussionsupporting

confidence: 73%

Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype

et al. 2013

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Background Cultured red blood cells (cRBCs) from cord blood (CB) have been proposed as transfusion products. Whether buffy-coats discarded from blood donations (AB) may be used to generate cRBCs for transfusion has not been investigated. Study Design and Methods Erythroid progenitor cell content and numbers and blood group antigen profiles of erythroblasts (ERYs) and cRBCs generated in Human Erythroid Massive Amplification (HEMA) culture by CB (n=7) and AB (n=33, three females, three males, one AB with rare blood antigens cryopreserved using CB protocols) were compared. Results Variability was observed both in progenitor cell content (2-fold) and number of ERYs generated (1-log) by CB and AB in HEMA. The average progenitor cell contents of the subset of AB and CB analyzed were similar. AB generated numbers of ERYs 3-times lower (p<0.01) than CB in HEMA containing fetal bovine serum but similar to CB in HEMA containing human proteins. Female AB contained 2-times less (p<0.05) erythroid progenitor cells but generated numbers of ERYs similar to those generated by male AB. Cryopreserved AB with a rare blood group phenotype and shipped to another laboratory generated great numbers of ERYs, 90% of which matured into cRBCs. Blood group antigen expression was consistent with the donor genotype for ERYs generated both by CB and AB but concordant with that of native RBCs only for cells derived from AB. Conclusion Buffy-coats from regular donors, including a donor with rare phenotypes stored under conditions established for CB, are not inferior to CB for the generation of cRBCs.

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Section: Discussionsupporting

confidence: 73%

Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype

et al. 2013

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“…As far as we know, this feature (the influence of donor sex on the number of all hematopoietic progenitors and stroma formation) has not been previously reported. However, a higher proportion of erythropoietic precursors in men has been related to the stimulatory effect of androgens [29]. We observed a lower CD34+ concentration in peripheral blood on day 5 in women compared to men.…”

Section: Resultsmentioning

confidence: 51%

Predictive factors that affect the mobilization of CD34+cells in healthy donors treated with recombinant granulocyte colony-stimulating factor (G-CSF)

et al. 2006

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No specific characteristics have been identified as predictors of peripheral blood stem cells (PBSC) mobilization in healthy donors. In this study, clinical characteristics and laboratory data for 122 healthy donors who underwent apheresis on day 5 of treatment with recombinant granulocyte colony-stimulating factor (G-CSF) were retrospectively analyzed for correlations with CD34(+) cell mobilization. The variables that were analyzed included age, sex, body weight, basal complete blood count, and maximum white blood count (WBC) before apheresis, G-CSF type, and dosage. Median age and body weight were 42.5 years (range 16-65) and 72.5 kg (range 47-121), respectively. By univariate analysis, male sex (P = 0.007), body weight (< or = 70 vs. >70 kg, P = 0.04), and donor's age (< or = 50 vs. > 50 years; P = 0.015) were correlated with the number of CD34(+) cells mobilized. By multivariate analysis, donor's age and male sex were the only two variables that significantly predicted a high CD34(+) cell level. In conclusion, our data suggest that male sex and younger age are the only factors that significantly affect CD34(+) mobilization in healthy donors.

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“…Specifically, male infants had significantly higher median CD34+ cell concentrations than female infants (31.8/μL compared with 30.2/μL, respectively; P = 0.03). This relative increase is reflected in adult males, who, when compared with age-matched females, have an increased number of colony-forming cells, erythroblastic colonies, and granulocytemacrophage colonies in their peripheral blood (56). This signifies that gender may affect the hematopoietic potential of cord blood transplants (57).…”

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

Sex Steroids and Stem Cell Function

Ray

Novotny

Crisostomo

et al. 2008

Mol Med

104

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Gender dimorphisms exist in the pathogenesis of a variety of cardiovascular, cardiopulmonary, neurodegenerative, and endocrine disorders. Estrogens exert immense influence on myocardial remodeling following ischemic insult, partially through paracrine growth hormone production by bone marrow mesenchymal stem cells (MSCs) and endothelial progenitor cells. Estrogens also facilitate the mobilization of endothelial progenitor cells to the ischemic myocardium and enhance neovascularization at the ischemic border zone. Moreover, estrogens limit pathological myocardial remodeling through the inhibitory effects on the proliferation of the cardiac fibroblasts. Androgens also may stimulate endothelial progenitor cell migration from the bone marrow, yet the larger role of androgens in disease pathogenesis is not well characterized. The beneficial effects of sex steroids include alteration of lipid metabolism in preadipocytes, modulation of bone metabolism and skeletal maturation, and prevention of osteoporosis through their effects on osteogenic precursors. In an example of sex steroid-specific effects, neural stem cells exhibit enhanced proliferation in response to estrogens, whereas androgens mediate inhibitory effects on their proliferation. Although stem cells can offer significant therapeutic benefits in various cardiovascular, neurodegenerative, endocrine disorders, and disorders of bone metabolism, a greater understanding of sex hormones on diverse stem cell populations is required to improve their ultimate clinical efficacy. In this review, we focus on the effects of estrogen and testosterone on various stem and progenitor cell types, and their relevant intracellular mechanisms.

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A statistically significant sex difference in the number of colony-forming cells from human peripheral blood

Cited by 11 publications

References 20 publications

Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype

Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype

Predictive factors that affect the mobilization of CD34+cells in healthy donors treated with recombinant granulocyte colony-stimulating factor (G-CSF)

Sex Steroids and Stem Cell Function

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