A statistical mechanical theory for the adsorption of protein to liposomal membranes

Suezaki, Yukio; Ichinose, Hiroyuki; Takiguchi, K.; Hotani, Hirokazu

doi:10.1016/s0301-4622(99)00065-4

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…Although our model is based on lecithin-bile salt mixtures, variants of the model could perhaps be applied to other systems, in particular other lipid-detergent mixtures (Jiskoot et al, 1986;Kaler et al, 1989;Edwards and Almgren, 1991;Silvander et al, 1996;O'Connor et al, 1997;Campbell et al, 1998;Brinkmann et al, 1998;Chen et al, 1999;Marques, 2000;Ollivon et al, 2000;Almgren, 2000;Xia et al, 2002;Schmölzer et al, 2002) or lipid-peptide mixtures (Dufourc et al, 1986;Saitoh et al, 1998;Suezaki et al, 1999), which undergo a similar micelle-to-vesicle transition. Because the parameters L,k k; and a m are independently accessible for various other systems, it should be possible to predict and understand their behavior based on the model we present here; we are currently investigating this.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of the Micelle-to-Vesicle Transition: Aqueous Lecithin-Bile Salt Mixtures

Leng

Egelhaaf

Cates

2003

Biophysical Journal

202

220

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Important routes to lipid vesicles (liposomes) are detergent removal techniques, such as dialysis or dilution. Although they are widely applied, there has been only limited understanding about the structural evolution during the formation of vesicles and the parameters that determine their properties. We use time-resolved static and dynamic light scattering to study vesicle formation in aqueous lecithin-bile salt mixtures. The kinetic rates and vesicle sizes are found to strongly depend on total amphiphile concentration and, even more pronounced, on ionic strength. The observed trends contradict equilibrium calculations, but are in agreement with a kinetic model that we present. This model identifies the key kinetic steps during vesicle formation: rapid formation of disk-like intermediate micelles, growth of these metastable micelles, and their closure to form vesicles once line tension dominates bending energy. A comparison of the rates of growth and closure provides a kinetic criterion for the critical size at which disks close and thus for the vesicle size. The model suggests that liposomes are nonequilibrium, kinetically trapped structures of very long lifetime. Their properties are hence controlled by kinetics rather than thermodynamics.

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Section: Discussionmentioning

confidence: 99%

Kinetics of the Micelle-to-Vesicle Transition: Aqueous Lecithin-Bile Salt Mixtures

Leng

Egelhaaf

Cates

2003

Biophysical Journal

202

220

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“…The author and co-workers successfully explained these observed data of cuplike vesicles by a statistical mechanical calculation. 18 This report is an extension of our theory to the mixture of lipid and talin.…”

Section: Introductionmentioning

confidence: 69%

“…The first three terms of the RHS of eq 17 are negative, a decreasing function of c and has a inflection point at c ) 2c th . 18 Thus, the formation of disclike micelles lowers the total free energy of the system, which has not been proven in the previous theoretical studies.…”

Section: Theoretical Formulationmentioning

confidence: 79%

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Theoretical Possibility of Cuplike Vesicles for Aggregates of Lipid and Bile Salt Mixture

Suezaki

2002

J. Phys. Chem. B

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The aggregate forms of bile salt and lipid mixture were studied by a statistical mechanical theory. The free energies of disclike mixed micelles, cuplike vesicles, and of rodlike micelles were defined and the minimized free energies were compared to study the stable forms of these aggregates. In addition to the adsorption affinity free energy of bile salt to the vesicle membrane, the following two energy terms were taken into account: one is the bending energies of the cuplike vesicle and rodlike micelle, and the other is the enhancement of the affinity free energies of bile salt from aqueous solvent to the curved membranes. By postulating the spontaneous curvature, which is assumed to be proportional to the surface coverage of bile salt, the cuplike vesicle and the rodlike micelle were shown to be mechanically frustrated. Despite the bending energy cost, the cuplike vesicle was shown to be the stable aggregate form in a certain concentration region. In this concentration region the molecular weight of the aggregate is expected to increase monotonically, while the radius of gyration is expected to possess a maximum value. This is consistent with the light scattering measurement made by Egelhaaf and Schurtenberger (J.

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“…This opening-up topological change was analyzed by a statistical theory [12], and was found to be due to the adsorption of talin to the verges of cup-shaped liposomes. The adsorption isotherm of talin between an aqueous solution and a liposome membrane was analyzed by taking into account the bending energy of the membrane.…”

Section: Opening-up Of Liposomesmentioning

confidence: 99%

Untitled

Nomura

Honda

Takeda

et al. 2002

Journal of Biological Physics

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Liposomes are micro-compartments made of lipid bilayer membranes withcharacteristics quite similar to those of biological membranes. To formartificial cell-like structures, we generated liposomes that containedsubunit proteins of cytoskeletons: tubulin or actin. Spherical liposomeswere transformed into bipolar or cell-like shapes by mechanical forcesgenerated by the polymerization of encapsulated subunits of microtubules.Disk- or dumbbell-shaped liposomes were developed by the polymerizationof encapsulated actin. Dynamic processes of morphological transformationsof liposomes were visualized by high intensity dark-field lightmicroscopy.Topological changes, such as fusion and division of membrane vesicles,play an essential role in cellular activities. To investigate themechanism of these processes, we visualized in real time the liposomesundergoing topological transformation. A variety of novel topologicaltransformations were found, including the opening-up of liposomes and thedirect expulsion of inner vesicles.

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A statistical mechanical theory for the adsorption of protein to liposomal membranes

Cited by 7 publications

References 13 publications

Kinetics of the Micelle-to-Vesicle Transition: Aqueous Lecithin-Bile Salt Mixtures

Kinetics of the Micelle-to-Vesicle Transition: Aqueous Lecithin-Bile Salt Mixtures

Theoretical Possibility of Cuplike Vesicles for Aggregates of Lipid and Bile Salt Mixture

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