Important routes to lipid vesicles (liposomes) are detergent removal techniques, such as dialysis or dilution. Although they are widely applied, there has been only limited understanding about the structural evolution during the formation of vesicles and the parameters that determine their properties. We use time-resolved static and dynamic light scattering to study vesicle formation in aqueous lecithin-bile salt mixtures. The kinetic rates and vesicle sizes are found to strongly depend on total amphiphile concentration and, even more pronounced, on ionic strength. The observed trends contradict equilibrium calculations, but are in agreement with a kinetic model that we present. This model identifies the key kinetic steps during vesicle formation: rapid formation of disk-like intermediate micelles, growth of these metastable micelles, and their closure to form vesicles once line tension dominates bending energy. A comparison of the rates of growth and closure provides a kinetic criterion for the critical size at which disks close and thus for the vesicle size. The model suggests that liposomes are nonequilibrium, kinetically trapped structures of very long lifetime. Their properties are hence controlled by kinetics rather than thermodynamics.
Many efforts have been devoted to the design and achievement of negative-refractive-index metamaterials since the 2000s. One of the challenges at present is to extend that field beyond electromagnetism by realizing three-dimensional (3D) media with negative acoustic indices. We report a new class of locally resonant ultrasonic metafluids consisting of a concentrated suspension of macroporous microbeads engineered using soft-matter techniques. The propagation of Gaussian pulses within these random distributions of 'ultra-slow' Mie resonators is investigated through in situ ultrasonic experiments. The real part of the acoustic index is shown to be negative (up to almost - 1) over broad frequency bandwidths, depending on the volume fraction of the microbeads as predicted by multiple-scattering calculations. These soft 3D acoustic metamaterials open the way for key applications such as sub-wavelength imaging and transformation acoustics, which require the production of acoustic devices with negative or zero-valued indices.
We use pervaporation-based microfluidic devices to concentrate species in aqueous solutions with spatial and temporal control of the process. Using experiments and modelling, we quantitatively describe the advection-diffusion behavior of the concentration field of various solutions (electrolytes, colloids, etc) and demonstrate the potential of these devices as universal tools for the kinetic exploration of the phases and textures that form upon concentration.PACS numbers: 07.90.+c, 64.75.+g Determination of the phase diagram of multicomponent systems is of importance in many realms: industrial formulation, protein cristallization, bottom up material assembly from spontaneous ordering of surfactant, polymeric or colloidal systems [1,2,3]. Depending on the application, one may want to access only the equilibrium phase diagram or gain additional information as to the metastable phases that can appear for kinetic reasons. Methods to reach these goals often imply tedious and systematic measurements, requiring for screening purposes the use of robotic platforms. Two generic strategies consist in varying (in space or time) the temperature of samples of given concentrations on the one hand, and on the other hand isothermal concentration by either removal of the solvent (osmosis, drying), external action on the solutes (sedimentation or dielectrophoresis for colloids), or studies of spontaneous interdiffusion in contact experiments.In this Letter we introduce microfluidic tools for controlled isothermal concentration of a wide range of systems, covering solutions of ions, polymers, proteins, surfactants and colloidal suspensions. Our work is inspired by recent observations [4,5] that in standard microsystems built of PolyDiMethylSiloxane (PDMS), spontaneous water permeation through the PDMS matrix induces flows that can be used to concentrate colloids. Taking a step further, we have engineered specialized microgeometries that allow us to control spatially and temporally the evaporation process as well as the resulting concentration of solutes. Their parallel implementation in microfluidic format could open the way to fast screening methods.After a brief description of the micro-devices, we demonstrate first our control of the concentration process on dilute aqueous solutions of fluorescein and nanoparticles in a simple geometry. We then discuss how microfabrication permits to widen the range of possibili- ties and applications of such devices. As a study case, we report controlled nucleation and growth of crystals of potassium chloride (KCl), and show how such experiments provide quantitative information on various thermodynamic quantities (solubility, crystal density) as well as kinetic features (sensitive to the rate of concentration).The devices -The devices used in this paper are twolayer PDMS on glass microsystems (Fig. 1) (fabrication procedure detailed in [6]). The microchannels of the bottom layer are filled with the solution of interest, while air (at controlled humidity) is circulated through the microchan...
Microfluidics offers a wide range of new tools that permit one to revisit the formation of crystals in solution and yield insights into crystallization processes. We review such recent microfluidic devices and particularly emphasize lab-on-chips dedicated to the high-throughput screening of crystallization conditions of proteins with nanolitre consumption. We also thoroughly discuss the possibilities offered by the microfluidic tools to acquire thermodynamic and kinetic data that may improve industrial processes and shed a new light on nucleation and growth mechanisms.
Raspberry-like magnetic nanoclusters are synthesized and self-assembled to form a bulk magnetic metamaterial.
Soft materials that embed small resonators in a host material can dampen or focus sound.
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