A statherin-derived peptide promotes hydroxyapatite crystallization and <i>in situ</i> remineralization of artificial enamel caries

Wang, Kun; Wang, Xiuqing; Li, Haoran; Zheng, Sainan; Ren, Qian; Wang, Yufei; Niu, Yumei; Zhou, Xuedong

doi:10.1039/c7ra12032j

Cited by 26 publications

(19 citation statements)

References 40 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of synthetic peptides, especially those derived from proteins in an enamel or dentin matrix, have been developed to promote remineralization of enamel and dentin [ 34 , 35 , 36 , 37 ]. Most were capable of inducing hydroxyapatite deposition, and a DSP-derived peptide was reported to act on pulp cells and induce osteodentin formation [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

et al. 2020

View full text Add to dashboard Cite

We aim to examine the effects of a newly developed peptide derived from CPNE7 (Cpne7-DP) in tertiary dentin formation and peritubular space occlusion, and comprehensively evaluate its potential as a bioactive therapeutic agent. Human dental pulp cells (HDPCs) and a mouse pre-odontoblast cell line, MDPC-23, were chosen for in vitro studies to characterize lineage-specific cell responses after Cpne7-DP treatment. Whether Cpne7-DP reproduces the dentin regenerative potential of CPNE7 was tested using a beagle dog model by generating dentinal defects of various degrees in vivo. Peritubular space occlusion was further examined by scanning electron microscopy and microleakage test, while overall mineralization capacity of Cpne7-DP was tested ex vivo. CPNE7 promotes tubular dentin formation under both shallow and deep dentinal defects, and the functional peptide Cpne7-DP induces odontoblast-like differentiation in vitro, mineralization ex vivo, and tubular dentin formation in in vivo beagle dog dentin exposure and pulp exposure models. Moreover, Cpne7-DP leads to peritubular space occlusion and maintains stability under different conditions. We show that CPNE7 and its derivative functional peptide Cpne7-DP promotes dentin regeneration in dentinal defects of various degrees and that the regenerated hard tissue demonstrates the characteristics of true dentin. Limitations of the current dental materials including post-operative hypersensitivity make biological repair of dentin a field of growing interest. Here, we suggest that the dual functions of Cpne7-DP in tubular dentin formation and peritubular space occlusion are promising for the treatment of dentinal loss and sensitivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Human oral keratinocytes (HOKs) (ATCC11303) were chosen to assess cell viability, which were derived from the stratum corneum of the oral mucosa, in order to study the cytotoxic effect of TDP (Liu et al, 2018; Wang et al, 2018). HOKs were cultured in alpha Dulbecco's Modified Eagle Medium containing 10% fetal bovine serum and 1% penicillin–streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

et al. 2020

Self Cite

View full text Add to dashboard Cite

With the gradual discovery of functional domains in natural proteins, several biologically inspired peptides have been designed for use as biomaterials for hard tissue regeneration and repair. In this study, we designed a tuftelin-derived peptide (TDP) and tested its effects on hydroxyapatite crystallization and remineralization of initial enamel carious lesions in vitro. Using circular dichroism spectroscopy, we found that TDP contained 36.1% β-sheets and β-turns, which could be influenced by calcium ions. We verified the ability of TDP to crystallize hydroxyapatite using transmission electron microscopy and its ability to bind to the enamel surface and hydroxyapatite using confocal laser scanning microscopy and Langmuir adsorption isotherms (K = 881.56, N = 1.41 × 10 −5). Artificial enamel lesions were generated on human enamel blocks and subjected to a 12-day pH cycling model and were treated with 25 μM TDP, 1 g/L sodium fluoride (NaF), or deionized water. We analyzed the results of remineralization by surface microhardness testing, polarized light microscopy, and transverse microradiography. The TDP group showed significantly higher surface microhardness recovery (49.21 ± 1.66%), shallower lesions (34.89 ± 4.05 μm), and less mineral loss (871.33 ± 81.49 vol%•μm) after pH cycling than the deionized water group (p < .05). There were no significant differences between the TDP and NaF groups. Our experiment indicated that TDP could regulate hydroxyapatite crystallization and promote remineralization of enamel caries in vitro.

show abstract

“…The concentrations of the peptide, TVH-19, in the solution, before and after incubation, were evaluated using a micro bicinchoninic acid (BCA) Protein Assay Kit (CWBio, Beijing, China) to calculate the amount of peptide bound to the unit surface area of the HA powder. Linear adsorption isotherms were then generated according to the Langmuir Equation (1) [32,33]. The maximum number of adsorption sites available for the protein per unit of the HA surface area and the affinity of the protein molecules for the HA adsorption sites were obtained from the resulting best-fit line:…”

Section: Binding Capacity Of Peptide To Hydroxyapatite (Ha)mentioning

confidence: 99%

The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization

et al. 2021

Self Cite

View full text Add to dashboard Cite

In this study, the amelogenin-derived peptide, TVH-19, which has been confirmed to promote mineralization, was evaluated to derive its potential to induce dentinal tubule occlusion. The binding capability of fluorescein isothiocyanate (FITC)-labeled TVH-19 to the demineralized dentin surface was analyzed by confocal laser scanning microscopy (CLSM). Additionally, the sealing function of the peptide was studied through the remineralization of demineralized dentin in vitro. The adsorption results showed that TVH-19 could bind to the hydroxyapatite and demineralized dentin surfaces, especially to periodontal dentin. Scanning electron microscopy analysis further revealed that TVH-19 created mineral precipitates. The plugging rate in the TVH-19 group was higher than that in the PBS group. Moreover, energy-dispersive X-ray spectroscopy (EDX) results indicated that the calcium/phosphorus (Ca/P) ratio of the new minerals induced by TVH-19 was close to that of the hydroxyapatite. Attenuated total internal reflection-Fourier transform infrared (ATR-FTIR) spectrometry and X-ray diffraction (XRD) results indicated that the hydroxyapatite crystals formed via remineralization elongated the axial growth and closely resembled the natural dentin components. These findings indicate that TVH-19 can effectively promote dentin sealing by binding to the periodontal dentin, promoting mineral deposition, and reducing the space between the dentin tubules.

show abstract

A statherin-derived peptide promotes hydroxyapatite crystallization and in situ remineralization of artificial enamel caries

Abstract: A novel biomimetic peptide inspired by salivary statherin was developed and exhibited beneficial potentials in promoting remineralization of hydroxyapatite on tooth enamel surface, providing a desirable alternative restorative strategy against dental caries.

Cited by 26 publications

References 40 publications

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization

Contact Info

Product

Resources

About