The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization

Peng, Xiaoyan; Han, Sili; Wang, Kun; Ding, Lei; Liu, Zhenqi

doi:10.3390/polym13152473

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…So it was used to detect the surface of the caries lesions and remineralization areas of all specimens and to further explore the crystal phase structure of the peptide TRAP synthesized for regulating the remineralization of enamel caries lesions in this study. Previous studies have demonstrated that the product of an amelogenin-derived peptide applied for remineralization was HA ( Ruan et al, 2014 ; Peng et al, 2021 ). The N-terminal tyrosine segment of amelogenin was extracted from LRAP and synthesized into a biomimetic enamel matrix protein using non-amelogenin analogs.…”

Section: Discussionmentioning

confidence: 99%

Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study

Qing-hua

Wen

et al. 2023

Front. Physiol.

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Objective: To explore the regulatory effect of recombinant amelogenin peptide TRAP on the remineralization of early enamel carious lesions.Methods: Forty-eight bovine enamel blocks that prepared initial lesions in vitro were split at random into four groups for immersion treatment for 12 days: 1) remineralizing medium; 2) studied peptide 1 (consisting of the N- and C-termini of porcine amelogenin) + remineralizing medium; 3) studied peptide 2 (TRAP) + remineralizing medium; 4) fluoride + remineralizing medium. After demineralization and remineralization immersion, each specimen’s mean mineral loss and lesion depth were measured using micro-computed tomography (micro-CT). The changes in lesion depth (∆LD) and mineral gain (∆Z) were computed following remineralization. The enamel samples were then cut into sections and examined with polarized light microscopy (PLM). The cross-section morphology was observed by scanning electron microscopy (SEM). The crystal phase was analyzed by an X-ray micro-diffractometer (XRD). The calcium-binding properties were determined using isothermal titration calorimetry (ITC).Results: Micro-CT analysis revealed a significant reduction in mineral loss in the four groups following the remineralization treatment (p < 0.05). The treatment with fluoride resulted in the greatest ∆Z and ∆LD, whereas the treatment with a remineralizing medium showed the least ∆Z and ∆LD among all groups. The ∆Z and ∆LD of the studied peptide 1 and studied peptide 2 groups were greater than those of the remineralizing medium group. However, there was no significant difference between the studied peptide 1 and studied peptide 2 groups (p > 0.05). All of the samples that the PLM analyzed had a thickening of the surface layer. A negative birefringent band changed in the lesion’s body. The SEM displayed that minerals were formed in all four groups of samples. The XRD results indicated that the products of remineralization of the studied peptide were hydroxyapatite crystals (HA). ITC showed that there were two binding modes between the calcium and peptide TRAP.Conclusion: This study confirmed the potential of the recombinant amelogenin peptide TRAP as a key functional motif of amelogenin protein for enamel remineralization and provided a promising biomaterial for remineralization in initial enamel carious lesion treatment.

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Section: Discussionmentioning

confidence: 99%

Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study

Qing-hua

Wen

et al. 2023

Front. Physiol.

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“…However, according to the literature, the active peptide structure may affect the morphology of the generated and deposited HA crystals (Mannem et al, 2020). In the experiments conducted by Xiu Peng's group, the deposits formed after remineralization of active and inactive peptides showed two different crystalline forms, namely flakes and needles (Peng et al, 2021). Therefore, in future studies, we should focus on the new HA morphology after remineralization of each active peptide to identify the most suitable active peptide for restoring the original morphology and function of dentin.…”

Section: Active Peptidesmentioning

confidence: 99%

Guided tissue remineralization and its effect on promoting dentin bonding

Yao

Chen

et al. 2022

Front. Mater.

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With the deepening of research on condensed matter chemistry, artificially guided demineralized dentin remineralization has changed from a classical remineralization pathway of the thermodynamic deposition mode to a biomimetic mineralization mode. This new mode is more consistent with the biological mineralization process. The biomimetic mineralization model can successfully simulate natural mineralization and restore the microstructure and mechanical properties of demineralized dentin. Therefore, it has a good application value in the treatment of caries and dentin hypersensitivity and adhesive restorations. This paper analyzes the principles of guided tissue remineralization and describes new research findings related to the classical mineralization model and the novel biomaterials developed using the biomimetic mineralization mode in detail. It also describes the application of these principles to improve the dentin bonding system. It thus shares the new findings in guided tissue remineralization applied to dentin bonding systems. Finally, the existing problems in this field and future development directions are proposed.

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“…Our study presents innovative findings on our previously developed amelogenin-derived peptide, TVH-19, which has been demonstrated as a multifunctional peptide for antibacterial, enamel remineralization, and inflammation prevention in dental pulp. − However, the precise mechanisms underlying these effects remain elusive. In this study, we first investigated the underlying mechanism of TVH-19 multifaced effects on DPCX based on its structure–activity relationship.…”

Section: Introductionmentioning

confidence: 99%

Amelogenin-Inspired Autoadaptive Peptide in a Caries Microenvironment Facilitates Long-Term Protection of the Dentin–Pulp Complex

Han,

Wang,

Peng

et al. 2024

ACS Appl. Mater. Interfaces

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Dental caries, one of the most prevalent infectious diseases, is the primary contributor to the early loss of natural teeth and is a significant public health issue. Known as the tooth's bioactive core, the dentin−pulp complex (DPCX) comprises tightly connected hard and soft tissues that not only serve as a biological barrier for the inner tooth tissue but also produce reparative dentin following mild disruptions. While efforts to preserve DPCX are numerous, most strategies focus on temporary antibacterial measures, inflammation reduction, or tissue regeneration, lacking a comprehensive, long-lasting solution. In this study, TVH-19, an autoadaptive peptide mimicking the pH-and ion-responsive capacity of amelogenin, was designed to exert multifaceted preservation of DPCX, providing a comprehensive strategy for preserving vital pulp. Leveraging its unique amphiphilicity-related cell penetration and ion/pH-responsive self-assembly properties, TVH-19 outperforms conventional pulp preservation materials by being capable of rapid cell penetration, minimizing diffused side effects, providing environment-responsive self-assembly/disassembly for balanced long-term antibacterial and cell protection, and facilitating the formation of lysosomal-escaping intracellular aggregates for the continuous activation of PDGFRα + dental pulp stem cells.

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The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization

Cited by 8 publications

References 47 publications

Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study

Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study

Guided tissue remineralization and its effect on promoting dentin bonding

Amelogenin-Inspired Autoadaptive Peptide in a Caries Microenvironment Facilitates Long-Term Protection of the Dentin–Pulp Complex

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