A standard protocol for obtaining fish chromosomes under post-mortem conditions

Netto, Maria Rita de Cáscia Barreto; Pauls, E.; Affonso, Paulo Roberto Antunes de Mello

doi:10.1016/j.micron.2006.07.019

“…After collection, the specimens were mitotically stimulated by inoculation of fungal antigens and kept in fish tanks for 24 to 72 h ( Lee and Elder 1980 ). After euthanasia in iced water ( Blessing et al 2010 ), the anterior kidney was removed and used to obtain mitotic chromosomes ( Netto et al 2007 , Blanco et al 2012 ). These procedures were approved by the Committee of Animal Ethics ( CEUA/UESB ) from Universidade Estadual do Sudoeste da Bahia (71/2014).…”

Section: Methodsmentioning

confidence: 99%

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Paim¹,

Almeida²,

Affonso³

et al. 2017

CCG

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The weakness of physical barriers in the marine environment and the dispersal potential of fish populations have been invoked as explanations of the apparent karyotype stasis of marine Percomorpha, but several taxa remain poorly studied cytogenetically. To increase the chromosomal data in this fish group, we analyzed cytogenetically three widespread Atlantic species from distinct families: Chaetodipterus faber Broussonet, 1782 (Ephippidae), Lutjanus synagris Linnaeus, 1758 (Lutjanidae) and Rypticus randalli Courtenay, 1967 (Serranidae). The three species shared a karyotype composed of 2n=48 acrocentric chromosomes, single nucleolus organizer regions (NORs) and reduced amounts of centromeric heterochromatin. A single NOR-bearing pair was identified in all species by physical mapping of 18S rDNA while non-syntenic 5S rRNA genes were located at centromeric region of a single pair. The similar karyotypic macrostructure observed in unrelated groups of Percomorpharia reinforces the conservative karyoevolution of marine teleosteans. Nonetheless, the species could be differentiated based on the pair bearing ribosomal cistrons, revealing the importance of microstructural analyses in species with symmetric and stable karyotypes.

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“…After collection, the specimens were mitotically stimulated by inoculation of fungal antigens and kept in fish tanks for 24 to 72 h (Lee and Elder 1980). After euthanasia in iced water (Blessing et al 2010), the anterior kidney was removed and used to obtain mitotic chromosomes (Netto et al 2007, Blanco et al 2012. These procedures were approved by the Committee of Animal Ethics (CEUA/UESB) from Universidade Estadual do Sudoeste da Bahia (71/2014).…”

Section: Methodsmentioning

confidence: 99%

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Paim

¹

,

Almeida

²

,

Affonso

³

et al. 2017

CCG

3

0

View full text Add to dashboard Cite

The weakness of physical barriers in the marine environment and the dispersal potential of fish populations have been invoked as explanations of the apparent karyotype stasis of marine Percomorpha, but several taxa remain poorly studied cytogenetically. To increase the chromosomal data in this fish group, we analyzed cytogenetically three widespread Atlantic species from distinct families: Chaetodipterus faber Broussonet, 1782 (Ephippidae), Lutjanus synagris Linnaeus, 1758 (Lutjanidae) and Rypticus randalli Courtenay, 1967 (Serranidae). The three species shared a karyotype composed of 2n=48 acrocentric chromosomes, single nucleolus organizer regions (NORs) and reduced amounts of centromeric heterochromatin. A single NOR-bearing pair was identified in all species by physical mapping of 18S rDNA while non-syntenic 5S rRNA genes were located at centromeric region of a single pair. The similar karyotypic macrostructure observed in unrelated groups of Percomorpharia reinforces the conservative karyoevolution of marine teleosteans. Nonetheless, the species could be differentiated based on the pair bearing ribosomal cistrons, revealing the importance of microstructural analyses in species with symmetric and stable karyotypes.

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“…Metaphase chromosomes were obtained from anterior kidney cells as described by Netto et al (2007) , without mitotic stimulation. Chromosome spreads were stained with 5% Giemsa in phosphate buffer for karyotyping.…”

Section: Methodsmentioning

confidence: 99%

ZZ/ZW sex chromosome system in the endangered fish Lignobrycon myersi Miranda-Ribeiro, 1956 (Teleostei, Characiformes, Triportheidae)

Rodrigues¹,

Medrado²,

Diniz³

et al. 2016

CCG

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Lignobrycon myersi is an endemic fish species from a few coastal rivers in northeastern Brazil. Based on molecular evidence, Lignobrycon myersi and genera Triportheus Cope, 1872, Agoniates Müller & Troschel, 1845, Clupeacharax Pearson, 1924 and Engraulisoma Castro, 1981 were placed in the family Triportheidae. In the present work, we report the first cytogenetic data for Lignobrycon myersi to test the hypothesis that Lignobrycon and Triportheus are closely related. Studied specimens presented 2n=52 with 28 (m)metacentric , 18 (sm)submetacentric and six (st)subtelocentric chromosomes for males and 27 m, 19 sm and 6 st for females, characterizing a ZZ/ZW sex chromosome system. The Z chromosome corresponds to the largest chromosome in karyotype while the W is about 50% smaller than the Z and largely heterochromatic. Terminal nucleolus organizer regions, GC-rich sites and 18S rDNA signals were detected on pair 14. However, additional 18S rDNA sites were observed in the W chromosome. The 5S rDNA was mainly detected on long arms of pair 7. The apparent synapomorphic chromosomal traits of Triportheus and Lignobrycon myersi reinforce their close phylogenetic relationship, suggesting that the ZZ/ZW chromosome system in both genera has arisen before cladogenic events.

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A standard protocol for obtaining fish chromosomes under post-mortem conditions

Cited by 25 publications

References 7 publications

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

ZZ/ZW sex chromosome system in the endangered fish Lignobrycon myersi Miranda-Ribeiro, 1956 (Teleostei, Characiformes, Triportheidae)

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