A Stable Starch Substrate for the Determination of Amylase in Serum and Other Body Fluids

Caraway, Wendell T.

doi:10.1093/ajcp/32.1_ts.97

Cited by 350 publications

(100 citation statements)

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“…Alkaline phosphatase (ALP) concentration was determined according to the colorimetric method of Bauer (1982) (Trinder, 1969) method using commercial kits. Plasma amylase activity was determined according to Caraway (1959). Serum immunoglobulin (Ig) fractions were determined according to Micini et al (1965).…”

Section: Methodsmentioning

confidence: 99%

Royal jelly: can it reduce physiological strain of growing rabbits under Egyptian summer conditions?

Elnagar

Elghalid

Abdelhady

2010

Animal

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Exposure of growing rabbits to heat stress during summer adversely affects their performance leading to major production losses. A total number of 48 rabbits, unsexed V-line weaned rabbits, were randomly divided into four experimental groups, temperature ranged from high at 328C to low at 238C. Animals of the 2nd, 3rd and 4th group were individually orally given 200, 400 or 800 mg royal jelly (RJ)/kg BW once a week, respectively, to evaluate RJ ability to reduce physiological strain resulted from heat stress. Weekly BW gain increased by 10.4, 11.8 and 10.8%, and feed conversion ratio was significantly improved by 20, 24 and 18% with RJ treatments. Serum total protein, albumin and globulin increased, whereas serum total lipids, cholesterol and triglycerides decreased with RJ treatments. Creatinine was reduced by 21, 30 and 18% and uric acid by 14, 25 and 18% compared with the heat stressed control with the three doses of RJ. Glucose level increased significantly to reach 116, 125, and 120% of heat stressed control. Calcium, phosphorus and alkaline phosphatase increased significantly with RJ treatments indicating the occurrence of active bone deposition. Thyroid hormone levels increased significantly to reach 108, 111, and 112% of heat stressed control rabbits with the three doses of RJ, counteracting the hypothyroid state resulted from heat stress. It can be concluded that RJ administration to heat stressed growing rabbits can reduce physiological strain resulted from heat stress.

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Section: Methodsmentioning

confidence: 99%

Royal jelly: can it reduce physiological strain of growing rabbits under Egyptian summer conditions?

Elnagar

Elghalid

Abdelhady

2010

Animal

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“…A atividade da amilase é inversamente proporcional à intensidade de cor azul e calculada pela comparação com um controle de substrato. A leitura de cor desenvolvida é realizada em espectrofotômetro a 660 nm, comprimento de onda em que apresenta maior estabilidade (Caraway, 1959).…”

Section: Methodsunclassified

“…A análise da atividade da amilase foi feita utilizando-se o kit enzimático da Bioclin, segundo a metodologia de Caraway (1959) modificada, conforme descrição a seguir. Em tubos previamente identificados (controle e teste), foram adicionados 250 µL do reagente n o 1.…”

Section: Methodsunclassified

Atividade da amilase em rã-touro durante a fase pós-metamórfica

Braga

Oliveira

Lima³

et al. 2005

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a atividade da amilase no quimo da rã-touro, Rana catesbeiana Shaw 1802 (3,6 a 200 g). Oitenta e sete animais foram distribuídos em baias-testes com temperatura e fotoperíodo controlados. As rãs selecionadas na fase pós-metamórfica receberam ração comercial extrusada ad libitum. Durante 87 dias de experimento, foram efetuadas 29 coletas em intervalos de 1 a 8 dias. As coletas do conteúdo intestinal foram feitas mediante a insensibilização das rãs em gelo e água e isolamento posterior do intestino delgado das mesmas. Para os testes da atividade da amilase, foram utilizados kits enzimáticos comerciais. A atividade da amilase foi observada a partir do terceiro dia e apresentou aumento até alcançar estabilidade, quando os animais pesavam 4,35 g. Do sétimo dia até o final do experimento, ocorreu a manutenção da estabilidade da atividade (137,44 UI) e atividade específica da amilase (14.688 UI mg -1 ).Termos para indexação: Rana catesbeiana, atividade enzimática, enzimas digestivas, ranicultura. Amylase activity in the bullfrog during the post-metamorphic phaseAbstract -The objective of this work was to evaluate the amylase activity in the chime of the bullfrog Rana catesbeiana Shaw 1802 (3.6 to 200 g). Eighty-seven animals were distributed in test stalls with controlled temperature and photoperiod. The frogs selected in the post-metamorphic phase received commercial extruded diet ad libitum. During 87 days of the experiment, 29 samples were collected in intervals varying from one to eight days. The intestinal content samples were made by first desensitizing of the frogs in ice and water, and subsequently removing the small intestine. For the tests of amylase activity, commercial enzymatic kits were used. Amylase activity was observed since the third day and it increased until reaching stability, when the animals weighed 4.35 g. Amylase activity (137.44 UI) and specific activity (14,688 UI mg -1 ) remained stable from the seventh day until the end of the experiment.Index terms: Rana catesbeiana, activity enzymatic, digestive enzymes, frogculture. IntroduçãoApesar da boa infra-estrutura disponível nos ranários, os produtores se deparam com vários obstáculos. O principal é o preço elevado da produção, e a ração é o item de maior custeio. Além disso, os ranicultores enfrentam problemas com a baixa produtividade, causada por instalações deficientes, manejo inadequado e sanidade dos animais (Lima et al., 2004).Para definir a formulação de rações compatíveis com as exigências nutricionais das rãs, nas diferentes fases de desenvolvimento, é preciso conhecer a fisiologia da digestão do animal. A eficiência de utilização dos alimentos está diretamente relacionada ao processo digestivo, no qual as enzimas têm papel fundamental. Neves (1996) detectou a presença precoce de proteases não-específicas no periblasto de larvas de trairão (Hoplias cf. lacerdae) e pacamã (Lophiosilurus alexandri). No estômago, a atividade ocorreu principalmente na região glandular, mas foi sempre menos inten...

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“…Each fraction was determined for the concentrations of Zn, Mg, amylase and HC03-, from which to calculate their outputs, the sum of values for 4 successive fractions being taken as daily output. The concentrations of Zn and Mg were determined by means of an atomic absorption flame spectrophotometry (Hackley et al 1968), while the concentrations of amylase and HC03-were measured by the methods of Caraway (1959) and Van Slyke et al (1919), respectively.…”

Section: Methodsmentioning

confidence: 99%