A spectrophotometric assay for superoxide dismutase activities in crude tissue fractions

Kuthan, Hartmut; Haussmann, Hans-Jürgen; Werringloer, J.

doi:10.1042/bj2370175

Cited by 94 publications

(44 citation statements)

References 23 publications

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“…SOD plays an important role in protecting the cell against the potentially toxic effects of environmental pollutants (Kuthan et al, 1986). In the present study, hepatic SOD activity was significantly decreased with the increasing dietary Cd levels.…”

Section: Enzyme Activities In Serum and Livermentioning

confidence: 95%

Toxic effects of two sources of dietborne cadmium on the juvenile cobia, Rachycentron canadum L. and tissue-specific accumulation of related minerals

et al. 2015

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Section: Enzyme Activities In Serum and Livermentioning

confidence: 95%

Toxic effects of two sources of dietborne cadmium on the juvenile cobia, Rachycentron canadum L. and tissue-specific accumulation of related minerals

et al. 2015

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“…Moreover, the authors reported that high-dose Ad.SOD2 gene delivery in rats prevented ischemia/reperfusion injury by inactivation of nuclear transcription factors NF-B and AP-1 (11). In contrast, in the liver transplantation model, Cu/Zn-SOD was selected because it represents the predominant isoform in the liver (12). Gene delivery of the Cu/Zn-SOD leads to a significant increase in SOD activity in transplanted livers, minimizes injury, and increases survival (9,10).…”

Section: Ischemia/reperfusion Injury Of Livers: Role Of Different Isomentioning

confidence: 98%

Effects of Three Superoxide Dismutase Genes Delivered With an Adenovirus on Graft Function After Transplantation of Fatty Livers in the Rat1

et al. 2003

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“…Next, the cytochrome C reduction rate was measured in the presence of the sample (23). The difference between the two rates was attributed to SOD being present in the tested sample.…”

Section: Animal Model and Experimental Design Eight-week-old Malementioning

confidence: 99%

High- versus moderate-intensity aerobic exercise training effects on skeletal muscle of infarcted rats

Moreira

Bechara

Bozi

et al. 2013

Journal of Applied Physiology

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Moreira JB, Bechara LR, Bozi LH, Jannig PR, Monteiro AW, Dourado PM, Wisløff U, Brum PC. High-versus moderate-intensity aerobic exercise training effects on skeletal muscle of infarcted rats. J Appl Physiol 114: 1029 -1041, 2013. First published February 21, 2013 doi:10.1152/japplphysiol.00760.2012.-Poor skeletal muscle performance was shown to strongly predict mortality and long-term prognosis in a variety of diseases, including heart failure (HF). Despite the known benefits of aerobic exercise training (AET) in improving the skeletal muscle phenotype in HF, the optimal exercise intensity to elicit maximal outcomes is still under debate. Therefore, the aim of the present study was to compare the effects of highintensity AET with those of a moderate-intensity protocol on skeletal muscle of infarcted rats. Wistar rats underwent myocardial infarction (MI) or sham surgery. MI groups were submitted either to an untrained (MI-UNT); moderate-intensity (MI-CMT, 60% V O2 max); or matched volume, high-intensity AET (MI-HIT, intervals at 85% V O2 max) protocol. High-intensity AET (HIT) was superior to moderate-intensity AET (CMT) in improving aerobic capacity, assessed by treadmill running tests. Cardiac contractile function, measured by echocardiography, was equally improved by both AET protocols. CMT and HIT prevented the MI-induced decay of skeletal muscle citrate synthase and hexokinase maximal activities, and increased glycogen content, without significant differences between protocols. Similar improvements in skeletal muscle redox balance and deactivation of the ubiquitin-proteasome system were also observed after CMT and HIT. Such intracellular findings were accompanied by prevented skeletal muscle atrophy in both MI-CMT and MI-HIT groups, whereas no major differences were observed between protocols. Taken together, our data suggest that despite superior effects of HIT in improving functional capacity, skeletal muscle adaptations were remarkably similar among protocols, leading to the conclusion that skeletal myopathy in infarcted rats was equally prevented by either moderate-intensity or high-intensity AET. atrophy; heart failure; proteasome; oxidative stress SKELETAL MUSCLE ABNORMALITIES in systemic diseases have been described for decades (9, 14, 29, 52), including in heart failure (HF). Capillary rarefaction, switch from type I (oxidative) to type II (glycolytic) fibers, impaired metabolism, and skeletal muscle atrophy have been shown in human and experimental models of HF (14,21,29,43,49,52). This maladaptation seems to impair skeletal muscle performance, which was proposed as the main determinant of exercise capacity in patients with HF (21,32,40). Additionally, skeletal muscle loss was shown to be an independent predictor of mortality in HF (3).Recent efforts by researchers have identified possible intracellular mechanisms underlying the skeletal muscle abnormalities in cardiovascular diseases, and promising targets such as metabolic enzymes, calcium handling-related proteins, antioxidant scavengers, and in...

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A spectrophotometric assay for superoxide dismutase activities in crude tissue fractions

Cited by 94 publications

References 23 publications

Toxic effects of two sources of dietborne cadmium on the juvenile cobia, Rachycentron canadum L. and tissue-specific accumulation of related minerals

Toxic effects of two sources of dietborne cadmium on the juvenile cobia, Rachycentron canadum L. and tissue-specific accumulation of related minerals

Effects of Three Superoxide Dismutase Genes Delivered With an Adenovirus on Graft Function After Transplantation of Fatty Livers in the Rat1

High- versus moderate-intensity aerobic exercise training effects on skeletal muscle of infarcted rats

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