A specific DNA sequence controls termination of transcription in the gastrin gene.

Sato, Kei; Ito, Ryoji; Baek, Kwanghee; Agarwal, Kan

doi:10.1128/mcb.6.4.1032

Cited by 93 publications

(67 citation statements)

References 47 publications

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“…Thus, Tat may act as a sequence-dependent antiterminator of transcription. These results are consistent with observations that the Tat responsive element (TAR) of the HIV-1 promoter functions in an orientation-and position-dependent manner (Rosen et al 1985a;Peterlin et al 1986;Muesing et al 1987), similar to that described for other eukaryotic terminator DNA segments (Zaret and Sherman 1984;Falck-Pedersen et al 1985;Sato et al 1986).…”

supporting

confidence: 80%

Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.

Jones¹,

Luciw²,

Duchange³

1988

Genes Dev.

270

190

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supporting

confidence: 80%

Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.

Jones¹,

Luciw²,

Duchange³

1988

Genes Dev.

270

190

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“…Experiments in other systems have provided evidence consistent with each of these ideas. In the h u m a n gastrin gene it appears that termination may occur at a single site (Back et al 1986;Sato et al 1986), whereas transcription of the mouse [3-globin (Citron et al 1984), dihydrofolate reductase (Frayne and Kellems 1986), and oL-amylase (Hagenbuchle et al 1984)probably occurs at multiple sites located within regions of several hundred base pairs. In contrast, no specific sites of termination have been detected during transcription of the polyoma virus late strand, and it has been suggested that termination of polyoma late transcription occurs at essentially random sites (Acheson 1984).…”

Section: Discussionmentioning

confidence: 99%

A functional mRNA polyadenylation signal is required for transcription termination by RNA polymerase II.

Connelly¹,

Manley²

1988

Genes Dev.

340

287

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Polyadenylation of pre-mRNAs requires the conserved hexanucleotide AAUAAA, as well as sequences located downstream from the poly(A) addition site. The role of these sequences in the production of functional mRNAs was studied by analyzing a series of mutants containing deletions or substitutions in the SV40 early region poly(A) site. As expected, both a previously defined GU-rich downstream element and an AAUAAA sequence were required for efficient usage of the wild-type poly(A) addition site. However, when either of these elements was deleted, greatly increased levels of SV40-specific RNA were detected in the nuclei of transfected cells' Evidence is presented that this accumulation of RNA resulted from a failure of transcription termination, leading to multiple rounds of transcription of the circular templates. We conclude that the sequences required for efficient cleavage/polyadenylation of the SV40 early pre-mRNA also constitute an important element of an RNA polymerase II termination signal. A model proposing a mechanism by which the act of pre-mRNA 3' end formation is signaled to the elongating RNA polymerase, resulting in termination, is presented.

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“…RNA polymerase II transcription termination occurs hundreds or thousands of nucleotides downstream of polyadenylation sites (7,13,14). Although there have been a number of reports of specific sequences which may act as termination sequences (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25), in vivo, transcription termination often depends on 3'-end processing (9)(10)(11)(12)26). Mutations in the conserved polyadenylation AAUAAA signal of the mouse major 3-globin gene or the human globin gene, respectively (11,12), or mutations in both the AAUAAA hexanucleotide and the downstream GT-rich region of the SV40 early polyadenylation site (9) can inhibit polyadenylation as well as transcription termination.…”

Section: Introductionmentioning

confidence: 99%

Polyadenylation and transcription termination in gene constructs containing multiple tandem polyadenylation signals

1994

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The processes of pre-mRNA 3'-end cleavage and polyadenylation have been closely linked to transcription termination by RNA polymerase II. We have studied the relationship between polyadenylation and transcription termination in gene constructs containing tandem poly(A) signals, at least one of which is the inefficient polyomavirus late poly(A) site. When identical tandem viral signals were separated by fewer than 400 bp, they competed for polyadenylation. The upstream site was always chosen preferentially, but relative site choice was influenced by the distance between the signals. All of these constructs showed the same low level of transcription termination as wild type polyomavirus, which contains a single late poly(A) site. When tandem poly(A) signals were not identical, a stronger downstream signal could outcompete a weaker upstream signal for polyadenylation without altering the efficiency of transcription termination characteristic for use of the upstream signal. Thus, if a weak polyoma virus late poly(A) signal (associated with inefficient transcription termination) preceded a strong rabbit 3-globin signal (associated with efficient transcription termination), termination remained inefficient, but the distal signal was most often chosen for polyadenylation. These results are consistent with independent regulation of polyadenylation and transcription termination in this system and are discussed in light of current models for the dependence of transcription termination on a functional poly(A) site.

show abstract

A specific DNA sequence controls termination of transcription in the gastrin gene.

Cited by 93 publications

References 47 publications

Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.

Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.

A functional mRNA polyadenylation signal is required for transcription termination by RNA polymerase II.

Polyadenylation and transcription termination in gene constructs containing multiple tandem polyadenylation signals

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