Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.

Jones, Katherine A.; Luciw, Paul A.; Duchange, Nathalie

doi:10.1101/gad.2.9.1101

Cited by 270 publications

(207 citation statements)

References 49 publications

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“…The filter was reprobed with tubulin and found to contain similar amounts of RNA in both lanes (data not shown). human c-myc promoters, the human immunodeficiency virus type 1 TATA element appears to be responsible for positioning transcription initiation (24).…”

Section: Resultsmentioning

confidence: 99%

Cloning and characterization of a human c-myc promoter-binding protein.

Ray¹,

Miller²

1991

Mol. Cell. Biol.

107

128

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A human cDNA clone encoding a c-myc promoter-binding protein was detected by screening a HeLa cell k phage expression cDNA library. The library was screened by using an XhoI-NaeI human c-myc P2 promoter fragment as a probe. The recombinant phage encoded a fusion protein, myc-binding protein 1 (MBP-1), which had an apparent molecular size of 40 kDa. A corresponding protein with a molecular size of 35 kDa was present in a HeLa cell extract. Sequence analysis of the cloned gene reveals an open reading frame of 1,038 bp with a 3' untranslated region of 378 bp. The predicted protein sequence contains a proline-rich region in the amino terminus but does not demonstrate a known DNA-binding domain. DNase I footprint analysis demonstrates that MBP-1 binds to the sequence just 5' of the TATA box sequence of the human c-myc P2 promoter. MBP-1 cDNA hybridizes to a 1.4-kb mRNA from HeLa and HL-60 cells, indicating that the cDNA insert (1,416 bp) is a full-length clone. Coexpression of the MBP-1 protein represses transcription from the human c-myc promoter, suggesting that MBP-1 may act as a negative regulatory factor for the human c-myc gene.

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Section: Resultsmentioning

confidence: 99%

Cloning and characterization of a human c-myc promoter-binding protein.

Ray¹,

Miller²

1991

Mol. Cell. Biol.

107

128

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“…These are as follows: (1) CP-2 (Lim et al, 1992(Lim et al, , 1993Shirra et al, 1994), also referred to as UBP-1, LSF, LBP-1c, and LBP-1d (alternatively spliced form), a widely expressed transcription factor that has been implicated in activation of the ␣-globin gene (Chodosh et al, 1988;Kim et al, 1990), the HIV-1 long terminal repeat (LTR; Jones et al, 1988;Wu et al, 1988;Malim et al, 1989;Kato et al, 1991), and the SV40 major late promoter (Kim et al, 1987;Huang et al, 1990). This factor can also, depending on context, repress transcription and was shown to cooperate with YY1 to repress HIV-1 LTR activity (Coull et al, 2000); (2) LBP-1a (Sueyoshi et al, 1995), also referred to as LBP-1b (alternatively spliced form) and NF2d9 (Yoon et al, 1994), a widely expressed factor implicated in regulation of the HIV-1 LTR (Yoon et al, 1994) and a P450 gene (Sueyoshi et al, 1995); (3) LBP-9 (Huang and Miller, 2000), also referred to as CP2-related transcriptional Repressor-1 (CRTR-1; Rodda et al, 2001), implicated in regulation of the P450 scc promoter; (4) LBP-32 (Huang and Miller, 2000), also refer- .…”

Section: Sequence Homology Of Mammalian Grainyhead-like Proteinsmentioning

confidence: 99%

Identification and characterization of Grainyhead‐like epithelial transactivator (GET‐1), a novel mammalian Grainyhead‐like factor

Kudryavtseva¹,

Sugihara²,

Wang³

et al. 2003

Developmental Dynamics

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LMO-4 is an LIM-only factor that is highly expressed in many epithelial cells, including those of the epidermis and hair follicles. Because LMOs may function by interacting with DNA-binding proteins, we have used the yeast two-hybrid system to screen mouse skin libraries for LMO-4 -interacting DNA-binding proteins. In this screen, we isolated a novel LMO-4 -interacting factor highly related to the Drosophila gene Grainyhead. Grainyhead is epidermally expressed and carries out important functions in cuticular formation in the fly embryo. With the identification of this novel mammalian Grainyhead-like gene, referred to as Grainyhead-like epithelial transactivator 1 (GET-1), the known members of the mammalian Grainyhead-like gene family are extended to six, falling into two classes based on sequence homology. Of interest, the expression pattern of GET-1 is similar to that of Drosophila Grainyhead with highest expression in the somatic ectoderm/epidermis, but GET-1 is additionally expressed in epithelial cells of gastrointestinal, genitourinary, and respiratory tracks. The GET-1 protein localizes to the nucleus and binds to at least one Grainyhead DNA-binding site. The GET-1 DNA-binding domain maps to a region containing homology to the Drosophila Grainyhead DNA-binding domain. GET-1 homodimerizes in solution by means of a short C-terminally located domain that is homologous to other Grainyhead-like genes. A short domain located between amino acids 100 and 190, which bears no homology to known transactivation domains, is sufficient to confer transactivation to the heterologous GAL4 DNA-binding domain. In addition, GET-1 appears to contain repression domains consistent with the observation that Grainyhead and other mammalian Grainyhead-like genes can act both as activators and repressors. These data suggest that GET-1 is a transcriptional regulator that may perform important functions in epithelial tissues of mammals. Developmental Dynamics 226:604 -617, 2003.

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“…To the best of our knowledge, only a few other mammalian promoters are known to have positive transcriptional elements located immediately downstream of the cap site (for references, see Jones et al 1988), and none of these bear any striking resemblance to the ribosomal protein promoters. The mouse c-myc gene has a positive element at the 3' end of exon I (Yang et al 1986), a region that also contains a binding site for the rpL30/ rpL32-8 factor (Atchison et al 1989).…”

Section: Importance Of Internal Elements For Ribosomal Protein Promotmentioning

confidence: 99%

Equipotent mouse ribosomal protein promoters have a similar architecture that includes internal sequence elements.

Hariharan¹,

Kelley²,

Perry³

1989

Genes Dev.

163

109

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The promoters of the mouse ribosomal protein genes rpL30, rpL32, and rpS16 are of equal strength, as indicated by in vivo measurements of polymerase loading and by their relative efficiency in driving the expression of a linked reporter gene. The equipotency of these promoters appears to derive from a remarkably similar architecture in which five or more elements are distributed over a 200-bp region that spans a polypyrimidineembedded cap site. Three trans-acting factors are shared by the rpL30 and rpL32 promoters, one of which, 8, recognizes a common CNGCCATCT motif in the first (untranslated) exons. Site-specific mutagenesis demonstrated that 8-factor binding is critical for rpL30 promoter function. The repeated occurrence of this novel promoter architecture among ribosomal protein genes with very different coding specificities is most readily explained by convergent evolution.

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Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.

Cited by 270 publications

References 49 publications

Cloning and characterization of a human c-myc promoter-binding protein.

Cloning and characterization of a human c-myc promoter-binding protein.

Identification and characterization of Grainyhead‐like epithelial transactivator (GET‐1), a novel mammalian Grainyhead‐like factor

Equipotent mouse ribosomal protein promoters have a similar architecture that includes internal sequence elements.

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