A specific combination of zeaxanthin, spermidine and rutin prevents apoptosis in human dermal papilla cells

Abstract

cer (30) give rise to future applications of 2D pH sensors in studying tumor metabolism. AcknowledgementsThis work was supported by grants from the German Research Foundation DFG (grants BA3410/4-1 and WO669/9-1). Traumasept Wound Gel was supplied by Dr. August Wolff GmbH & Co. KG (Bielefeld, Germany). Author contributionsS.S., R.J.M., O.S.W. and P.B. conceived the experiments. S.S., R.J.M., K.T.W., J.C. and D.F. performed the experiments. S.S., R.J.M. and D.F. analysed the data. S.S. and R.J.M. wrote the paper. S.G. helped to interpret data. Conflict of interests Supporting InformationAdditional Supporting Information may be found in the online version of this article: Figure S1. Region of interest in a split skin donor site wound. Figure S2. Demonstration of complete sensor removal. Figure S3. Visualization of pH heterogeneity in wounds. Figure S4. Visualization of bacteria-induced pH changes. , via A. di Rudinì 8, 20142 Milan, Italy, Tel.: +390250323032, Fax: +390250323033, e-mail: alfredo.gorio@unimi.it Abstract: Hair follicle (HF) regression is characterized by the activation of apoptosis in HF cells. Dermal papilla cells play a leading role in the regulation of HF development and cycling. Human follicular dermal papilla cells (HFDPC) were used to investigate the protective activities of rutin, sperimidine and zeaxanthine. HFDP cell incubation with staurosporine caused apoptosis, which was completely inhibited by exposure to rutin (2.2 lM), spermidine (1 lM) and zeaxanthin (80 lM). These agents were much less effective when applied as single compounds. Moreover, treatment preserved the expression of anti-apoptotic molecules such as Bcl-2, MAP-kinases and their phosphorylated forms. In conclusion, the investigated agents may represent an effective treatment for the prevention of apoptosis, one of the leading events involved in hair bulb regression.

Zeaxanthin Dipalmitate Therapeutically Improves Hepatic Functions in an Alcoholic Fatty Liver Disease Model through Modulating MAPK Pathway

“…ZD treatment evidently reduced the apoptotic ratio through restoring Bcl-2 expression and reducing caspase activity. These data supported the beneficial effects of ZD on the liver by improving the anti-apoptotic response, which was consistent with a recent study showing the anti-apoptotic function of zeaxanthin through Bcl-2 and MAPK in human dermal papilla cells [30].…”

The Pharmacological Potential of Rutin

“…In a study, apoptosis of human follicular dermal papilla cells was observed after treatment with staurosporine; this event was completely introverted by exposure to rutin, spermidine, and zeaxanthin. There was the preservation of expression of anti-apoptotic molecules such as Bcl-2, MAP-kinases and their phosphorylated forms, which concludes the use of rutin in the prevention of apoptosis of hair follicular cells, one of the leading causes of baldness (Carelli et al, 2012).…”

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

“…The research on herbs and active constituents as hair growth promoters has been driven by their less toxicity, easy availability, low‐cost, patient compliance and more than one mode of biochemical action . Specific effects of herbal extracts, polyherbal formulations, flavonoids and natural compounds on DPCs have been examined . In vivo hair growth promoting effects as proof of concept are also mentioned.…”