A specific and biocompatible fluorescent sensor based on the hybrid of GFP chromophore and peptide for HSA detection

Liao, Caiyun; Li, Fangfang; Huang, Shanshan; Zheng, Baozhan; Du, Juan; Xiao, Dan

doi:10.1016/j.bios.2016.07.002

Cited by 44 publications

(6 citation statements)

References 32 publications

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“…The dual-self-restricted chromophores were designed to be functionalizable at multiple positions via different chemical synthesis approaches. With an enhanced fluorescence response of the variable emission color palette, these chromophores are suitable for the development of functional fluorescent probes for molecular and cellular applications. − …”

Section: Discussionmentioning

confidence: 99%

Dual-Self-Restricted GFP Chromophore Analogues with Significantly Enhanced Emission

Deng

Yan

et al. 2020

J. Phys. Chem. B

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The tremendous gap of fluorescence emission of synthetic green fluorescent protein (GFP) chromophore to the protein itself makes it impossible to use for applications in molecular and cellular imaging. Here, we developed an efficient methodology to enhance the photoluminescence response of synthetic GFP chromophore analogues by constructing dual-self-restricted chromophores. Single self-restricted chromophores were first generated with 2,5-dimethoxy substitution on the aromatic ring, which were further modified with phenyl or 2,5-dimethoxy phenyl to form dual-self-restricted chromophores. These two chromophores showed an obvious solvatofluorochromic color palette across blue to yellow with a maximum emission Stokes shift of 95 nm and dramatically enhanced fluorescence emission in various aprotic solvents, especially in hexane, where the QY reached around 0.6. Importantly, in acetonitrile and dimethyl sulfoxide, the fluorescence QYs of both chromophores were over 0.22, which were the highest reported so far in high polar organic solvents. Meanwhile, the fluorescence lifetimes also improved obviously with the maximum of around 4.5 ns. Theoretical calculations revealed a more favorable Mülliken atomic charge translocation over the double-bond bridge and illustrated much higher energy barriers for the Z/E photoisomerization together with larger bond orders compared with the GFP core chromophore, p-HBDI. Our work significantly improved the fluorescence emission of synthetic GFP chromophore analogues in polar solvents while reserved the multicolor emitting function, providing a solid molecular motif for engineering high-performance fluorescent probes.

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Section: Discussionmentioning

confidence: 99%

Dual-Self-Restricted GFP Chromophore Analogues with Significantly Enhanced Emission

Deng

Yan

et al. 2020

J. Phys. Chem. B

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“…Up until now, some methods, such as fluorescence-based assays, antibody based methods, LC–MS/MS, and so on, have been developed for HSA quantitative detection in blood samples. , Among them, fluorescence assays have been widespread applied to quantify HSA in multiple samples samples, due to their favorable perpertied for the rapid, convenient, sensitive, and nondestructive detection for biological samples. − However, the reported fluorescent bioprobes for detection of HSA possess a few limitations. For example, some bioprobes are insoluble in aqueous media, − which makes it difficult for assays in biological systems; a few bioprobes selectively recognize protein by covalent reactions with the thiol or amino moiety of the protein; however, this irreversible binding may lead to denaturation of proteins. , What is more, some bioprobes for protein detection comprising conventional organic dye molecules tend to aggregate when dispersed in physiological buffer, which usually quenches fluorescence attributed to the aggregates’ nonradiative relaxation. − Limitations of probes for protein detection listed above has forced researchers to employ single molecules in extreme dilute solutions as bioprobes. However, the dilute solution may lead to a weak emission and the poor sensitivity in fluorescent bioassays.…”

mentioning

confidence: 99%

Smart Self-Assembled Organic Nanoprobe for Protein-Specific Detection: Design, Synthesis, Application, and Mechanism Studies

et al. 2017

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Specific detection or imaging protein has high potential to contribute greatly to medical diagnosis, biological research, and therapeutic applications. The level of human serum albumin (HSA) in blood is related to a variety of diseases and thus serves as an important biomarker for fast clinical diagnosis. Here we report the use of aggregation-induced emission (AIE) based supramolecular assembly to design biomolecular responsive smart organic nanomaterials for detection protein HSA. The designed nanoprobes were aggregates of small molecules and silent in fluorescence, but in the presence of HSA they disassembled and produced a clear turn-on fluorescent signal. Of a small library of nanoprobes constructed for HSA detection, structure-optical signaling and screening studies revealed that nanoprobe 7 is the most efficient one. Mechanism studies showed that nanoprobe 7 was bonded with Site I of HSA through the multiple noncovalent interactions. The resultant restriction of intramolecular rotation of nanoprobe 7 in the hydrophobic cavity of HSA induced fluorescent emission, which was validated by competitive binding assays and molecular docking. More importantly, nanoprobe 7 was successfully applied to recognize and quantify HSA in human serum samples. This study demonstrates nanoprobe 7 is a promising tool for clinical real and fast detection of HSA and thus may find many applications, and the molecular assembly based on AIE also opens a new avenue for designing smart nanomaterials for the sensitive and selective detection for varied analytes.

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“…The importance of the serum albumins has triggered studies of their direct detection in serum or in urine, using various luminescent probes. [7][8][9] The presence of HSA in urine signals the onset of renal malfunction, whilst a deficiency in blood is associated with liver disease, e.g. cirrhosis and chronic hepatitis.…”

mentioning

confidence: 99%

Selectively switching on europium emission in drug site one of human serum albumin

2017

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A specific and biocompatible fluorescent sensor based on the hybrid of GFP chromophore and peptide for HSA detection

Cited by 44 publications

References 32 publications

Dual-Self-Restricted GFP Chromophore Analogues with Significantly Enhanced Emission

Dual-Self-Restricted GFP Chromophore Analogues with Significantly Enhanced Emission

Smart Self-Assembled Organic Nanoprobe for Protein-Specific Detection: Design, Synthesis, Application, and Mechanism Studies

Selectively switching on europium emission in drug site one of human serum albumin

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