Selectively switching on europium emission in drug site one of human serum albumin

Abstract

A luminescent europium probe has been discovered that binds selectively to drug-site I in human serum albumin, signalled by a 'switching on' of europium emission, and accompanied by strong induced circularly polarised luminescence.

A Smart Sensing Method for Object Identification Using Circularly Polarized Luminescence from Coordination‐Driven Self‐Assembly

“…[6,7] In most cases,high affinity to the target, longer wavelength detection, or time-gating detection excludes as ignal from non-target species.Onthe other hand, circularly polarized luminescence has been used in an optical technique to prove the excitedstate chirality, [8][9][10][11][12] but has yet to be fully exploited in ac onventional sensor application for achiral analytes. [6,7] In most cases,high affinity to the target, longer wavelength detection, or time-gating detection excludes as ignal from non-target species.Onthe other hand, circularly polarized luminescence has been used in an optical technique to prove the excitedstate chirality, [8][9][10][11][12] but has yet to be fully exploited in ac onventional sensor application for achiral analytes.…”

“…Herein, we propose ap otential application of circularly polarized luminescence for object identification in as ensor technology.L uminescence sensors are one of the common analytic tools for detection of the target analytes. [6,7] In most cases,high affinity to the target, longer wavelength detection, or time-gating detection excludes as ignal from non-target species.Onthe other hand, circularly polarized luminescence has been used in an optical technique to prove the excitedstate chirality, [8][9][10][11][12] but has yet to be fully exploited in ac onventional sensor application for achiral analytes. Scheme 1s hows an outline of the procedure for the CPLdriven luminescent sensing system demonstrated here.Inthe present system, the chiral probe exhibits circularly polarized luminescence (CPL, I L ¼ 6 I R )u pon binding to the target analyte.C onversely,n on-target species show non-polarized luminescence (NPL, I L = I R ), which can be eliminated by CPL measurement because non-polarized luminescence can be considered as equal amount of left and right circularly polarized light (that is, I L ÀI R = 0).…”

Molecular neurochemistry of the lanthanides

“…Europium‐superparamagnetic iron oxide particles may be also useful for recent studies on the role of the brain choroid plexus in the mechanism of monocyte movement during neuroinflammatory processes (Milward et al ). Europium probes can bind selectively to some drug sites on human serum proteins that suggests their potential usefulness in current diagnostics and basic pharmacological studies (Shuvaev, Pal, & Parker, ).…”

Monitoring of the ADP/ATP Ratio by Induced Circularly Polarised Europium Luminescence

“…[5,6] Different strategies to modulate selectivity and binding affinities have been employed, including steric complementarity, [7] variation of the overall charge, [8] and introduction of stabilising and orienting weak interactions,such as hydrogen bonding, [9] p-p stacking, [10] or boroncarbohydrate interactions. Such g em values are about two orders of magnitude higher than in organic molecules or transition-metal complexes.Incomparison with circular dichroism (CD) spectroscopy,C PL spectroscopy provides higher sensitivity,a nd its ability to resolve transitions otherwise invisible by conventional luminescence spectroscopy renders it advantageous over total emission spectral analysis.S trong CPL signals of europium and terbium complexes have been exploited to create pH, [13] temperature, [14] protein, [15][16][17][18] and anion-sensitive [19][20][21][22] CPL probes,w hose dissymmetry factor has been monitored as afunction of pH or added analyte.Deviations in cellular uptake of different enantiomers can also be exploited, with the advent of chiral luminescence microscopy,b y detecting the CPL signal from enantiomeric complexes in cellulo. Such g em values are about two orders of magnitude higher than in organic molecules or transition-metal complexes.Incomparison with circular dichroism (CD) spectroscopy,C PL spectroscopy provides higher sensitivity,a nd its ability to resolve transitions otherwise invisible by conventional luminescence spectroscopy renders it advantageous over total emission spectral analysis.S trong CPL signals of europium and terbium complexes have been exploited to create pH, [13] temperature, [14] protein, [15][16][17][18] and anion-sensitive [19][20][21][22] CPL probes,w hose dissymmetry factor has been monitored as afunction of pH or added analyte.Deviations in cellular uptake of different enantiomers can also be exploited, with the advent of chiral luminescence microscopy,b y detecting the CPL signal from enantiomeric complexes in cellulo.…”

“…[11] Furthermore,t he high rotatory strength [12] of certain magnetic-dipole allowed transitions in Eu 3+ complexes can give rise to as trong circularly polarised luminescence (CPL) signal, with adissymmetry factor (g em )of up to 1.4. Such g em values are about two orders of magnitude higher than in organic molecules or transition-metal complexes.Incomparison with circular dichroism (CD) spectroscopy,C PL spectroscopy provides higher sensitivity,a nd its ability to resolve transitions otherwise invisible by conventional luminescence spectroscopy renders it advantageous over total emission spectral analysis.S trong CPL signals of europium and terbium complexes have been exploited to create pH, [13] temperature, [14] protein, [15][16][17][18] and anion-sensitive [19][20][21][22] CPL probes,w hose dissymmetry factor has been monitored as afunction of pH or added analyte.Deviations in cellular uptake of different enantiomers can also be exploited, with the advent of chiral luminescence microscopy,b y detecting the CPL signal from enantiomeric complexes in cellulo. [23] Adenosine phosphates are naturally chiral anions,a nd they can give rise to as ignal that can be detected by chiroptical methods.I th as been shown, for example,t hat ADP and ATPm ay produce signals of opposite handedness once they are added to az inc complex by forming ah elical oligomer.…”