A Specific Anamnestic Immune Response Induced by Plant Mitogens

Mãkelä, O.; Pasanen, V. J.

doi:10.1159/000230199

Cited by 4 publications

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Effect of phytohemagglutinin on thymidine incorporation and antibody production in the secondary response in vitro towards a defined hapten

et al. 1971

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The Weizmann Institute of Science, RehovotRabbits were immunized with 2,4-dinitrophenyl bovine serum albumin (DNP-BSA), and the spleen cells were removed and cultured in uitro in the presence of either phytohemagglutinin (PHA) or antigen, or both stimulants.PHA and antigen, when reacted together, had an additive stimulatory effect on thymidine uptake and an inhibitory effect on the production of antibodies as compared to the effect of the antigen alone. Cultures treated with PHA alone contained more antibodies than unstimulated cultures.

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Effect of phytohemagglutinin on thymidine incorporation and antibody production in the secondary response in vitro towards a defined hapten

et al. 1971

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Anti‐hapten antibodies in normal sera

Jormalainen

Mãkelä

1971

Eur J Immunol

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Many normal human, rabbit and reindeer sera were found to inactivate bacteriophages coupled with synthetic haptens NIP (4-hydroxy-3-iodo-5-nitrophenylacetic acid), NP (hydroxy-3-nitrophenylacetic acid), DNP (2,4-dinitrophenol), oxazolone (2-phenyl-4-ethoxy methylene oxazolone) or penicillin G. Evidence was produced indicating that these inactivators are antibodies with specificities roughly as strict as those of early immune antibodies. While most of the phage-inactivation power was due t o 19 S antibodies, 7 S inactivators with chromatographic characteristics of IgG were demonstrated.There were great inter-individual differences in various anti-hapten titers. In spite of that, inter-species differences could also be demonstrated. ntroductionMany mammalian sera contain antibodies that cannot be connected with the known immunization history of the individual. These are called "natural antibodies" in this series of papers [ 11. We are not including factors that are probably not immunoglobulins, such as lobster serum agglutinins [2] or heat-labile (56 OC, 30 min) agents. Claims that heat-labile natural antibodies exist may have originated from the fact that heat-labile complement can greatly enhance the activity of heat-stable antibodies. Well known natural antibodies are ABO-isoagglutinins and several antibacterial factors (for review see Boyden, [3]). S . Jormalainen and 0. MakelaEur.

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