The Regulatory Influence of Activated T Cells on B Cell Responses to Antigen

125

Regulatory effects of alloantigen-activated thymus-derived lymphocytes in mixed lymphocyte reactions have been demonstrated. Mice were injected into foot pads with allogeneic spleen cells; 4 days following sensitization spleen or regional lymph node cells from these animals were treated with mitomycin C and incorporated into MLR as regulator populations syngeneic to the responder cell type. Activated spleen cells suppressed MLR responses 60–90% whereas activated lymph node cells from the same animals enhanced MLR responses. Suppression by activated spleen cells was not due to cytotoxic effects nor to altered kinetics of the proliferative response. Studies of splenic suppressor cell generation in vivo revealed peak activity four days after alloantigen stimulation with no activity demonstrable at 7 days or at later times. Suppressor cell activity was abrogated by treatment with anti-θC3H serum and complement, and was not alloantigen specific.

Section: Discussionmentioning

confidence: 99%

Regulatory Mechanisms in Cell-Mediated Immune Responses

Rich

1974

125

“…6 days later, these recipient mice and groups of similarly primed control mice which had received no allogeneic cell transfer were subjected to secondary challenge intraperitoneally with either 100 #g of DNP-KLH or varying doses (10,50, or 100/zg) of the heterologous conjugate, DNP-BGG.…”

Section: Elicita$ion Of the Allegeneic Effect In Inbredmentioning

confidence: 99%

“…The demonstration that induction of a transient graftversus-host reaction can, under appropriate circumstances, markedly enhance specific antibody production in a previously primed animal (1) has provided a useful model with which to approach this problem. Thus, the allogeneic effect, whose properties are discussed briefly in the introduction, and studies of other investigators using somewhat different systems (11,12), have provided strong evidence for a hypothesis which considers T cell regulation of B cell function to be mediated through release of a soluble nonspecific factor(s) from appropriately activated T lymphocytes (10).…”

Section: Dnp-ova Dnp'taa Dnp'd'gl Dnp-d-glmentioning

confidence: 99%

The Allogeneic Effect in Inbred Mice

Osborne

Katz

1972

Self Cite

In recent studies (1-3) it has been demonstrated that the passive transfer of imrnunocompetent allogeneic lymphoid cells to guinea pigs previously primed with 2,4-dinitrophenyl-ovalbumin (DNP-OVA) 1 results in the increased synthesis of anti-DNP and anti-OVA antibodies in the absence of further antigenic challenge, and markedly enhances anti-DNP responses to an appropriately timed challenge with DNP coupled to an unrelated carrier, bovine gamma globulin (BGG). This phenomenon has been termed the "allogeneic effect" and has several salient features. First, the phenomenon reflects and requires the development of a transient graftversus-host (GVtt) reaction in the lymphoid organs of the primed host (1). The existence of a concomitant host rejection reaction is not only not required, but appears to play little if any role (3). Secondly, the lymphoid cells of the host must be primed before the administration of allogeneic cells. Numerous attempts to demonstrate an enhanced primary response by the prior administration of allogeneic cells have failed, and, indeed, the primary response is usually suppressed under these circumstances (1-3a). Finally, the allogeneic effect has been shown to replace the requirement for carrier-specific helper T cells in the development of hapten-specific anamnestic antibody responses manifested by the primed lymphoid cells of the host (2).The studies presented in this and the accompanying paper (4) were undertaken in inbred mice to elucidate further the phenomenon of the allogeneic effect, in that, as a model, the mouse provides greater flexibility in manipulating cell populations and histocompatibility differences. In the experiments described here, we show that: (a) the general features of the allogeneic effect

“…It is known that T cells perform a helper function in production of humoral immunity (12). A genetic difference in T-cell function can, therefore, produce an apparent difference in B-cell function.…”

mentioning

confidence: 99%

GENETIC CONTROL OF THE ANTIBODY RESPONSE TO POLY-L(TYR,GLU)-POLY-D,L-ALA--POLY-L-LYS IN C3H↔CWB TETRAPARENTAL MICE

Bechtol

Freed

Herzenberg

et al. 1974

In order to further delineate the mechanisms underlying genetic unresponsiveness, tetraparental mice were constructed from immune response-1A gene high responder and low responder parental genotypes, then were immunized with poly-L-(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys ((T,G)-A--L). An analysis of the total serum allotype mixture and of the antigen-binding capacity of the separated allotypes demonstrated that in the milieu of a tetraparental mouse, both high and low responder B cells could be stimulated equally to produce identical high titered anti-(T,G)-A--L responses. Furthermore, these studies show that effective stimulation could occur across a histocompatibility disparity.