A solution hybridization method for quantification of mRNAs: Determining the amount and stability of oncogene mRNA

Tenhunen, Jukka; Eloranta, Jyrki J.; Kallio, Arja

doi:10.1016/0735-0651(90)90005-z

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Cited by 6 publications

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“…Use of high concentrations of either cell lysate or total RNA caused a decrease of the fluorescence signal possibly due to unspecific binding. With low cellular levels of target RNA more cell material is required in the assay, and correspondingly, then inhibition has to be considered in signal quantification [20]. Thus the amount of cell lysate used in hybridization assay should be optimized for routine analysis.…”

Section: Discussionmentioning

confidence: 99%

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et al. 2003

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Background: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated by alkaline phosphatase. The 18S rRNA and SUC2 mRNA of Saccharomyces cerevisiae were used as model RNA target molecules.

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Section: Discussionmentioning

confidence: 99%

“…1). Sandwich hybridization is relatively sensitive and can be performed with crude biological samples [20]. …”

Section: Introductionmentioning

confidence: 99%