1974
DOI: 10.1042/bj1430219
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A solid-phase radioimmunoassay for plasma progesterone

Abstract: A detailed procedure is presented for the assay of plasma progesterone. The routine assay is based on the use of antiserum which is covalently linked to microcrystalline cellulose, the double-antibody method being used as a reference separation system. This procedure gives high precision accompanied by small and acceptable losses of antiserum titre but without loss of sensitivity when compared with the double-antibody method. Ethanol is first added to the plasma (10vol. of plasma+1vol. of ethanol) after which … Show more

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Cited by 43 publications
(27 citation statements)
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“…Progesterone was measured by radioimmunoassay using an antibody raised and tested by Dighe & Hunter (1974) and assessed for its suitability for use in the laboratory by Poyser & Horton (1975). The antibody had adequate sensitivity (limit 20 pg) and specificity (see Table 1 in Poyser & Horton, 1975) and the results obtained in the present study were in the expected range.…”
Section: Assay Proceduresmentioning
confidence: 66%
“…Progesterone was measured by radioimmunoassay using an antibody raised and tested by Dighe & Hunter (1974) and assessed for its suitability for use in the laboratory by Poyser & Horton (1975). The antibody had adequate sensitivity (limit 20 pg) and specificity (see Table 1 in Poyser & Horton, 1975) and the results obtained in the present study were in the expected range.…”
Section: Assay Proceduresmentioning
confidence: 66%
“…Progesterone concentrations were measured in a single-antibody solid-phase assay, similar to that described by Dighe & Hunter (1974), using a specific sheep antiprogesterone serum (Baird, Burger, Heavon-Jones & Scaramuzzi, 1974). All standards were diluted with buffer containing charcoal-treated calf plasma before extraction, so that standards and unknowns contained the same amount of plasma.…”
Section: Animals and Experimentsmentioning
confidence: 99%
“…la-hemisuccinate-bovine serum albumin (BSA) conjugate. The specificity has been described by Dighe & Hunter (1974). Antibody to testosterone was raised in a goat immunized with testosterone-3-carboxymethyl oxime conjugated to BSA.…”
Section: Methodsmentioning
confidence: 99%