A solid phase enzyme immunoassay for the quantitation of serum ferritin

Lee, Myung; Burgett, Michael W.

doi:10.1016/0009-8981(81)90383-1

Cited by 10 publications

(1 citation statement)

References 13 publications

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“…With the BNA, the present assay is more convenient and requires less analysis time than required to perform the radioimmunoassay or ELISA methods. Sensitivity of the present method is good and comparable to other immunoassays systems (21,22). The high volume of sample utilized is associated with interferences due to endogenous turbidity and this method requires sample pretreatment of turbid or lipemic samples as reported for other nephelometric latex tests (23,24).…”

Section: Discussionmentioning

confidence: 54%

Automated quantitative nephelometric latex immunoassay for determining ferritin in human serum

Borque

Rus

Cura

et al. 1992

Clinical Laboratory Analysis

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We describe a rapid and sensitive latex nephelometric immunoassay for quantifying ferritin in human serum. This latex immunoassay procedure uses commercially available ready-for-use reagents [Tina-Quant (a) Ferritin, Boehringer Mannheim] that have a long shelf life. The assay consists of incubating the diluted serum sample (5-fold) for 12 min at room temperature with latex particles covalently coated with anti-ferritin antibodies, and then quantifying the change of light-scatter produced. The assay is fully automated on the Behring nephelometer analyzer with a sampling rate of 150 samples/hour. The method has an analytical range of 3 to 260 micrograms/l. Maximal intra- and inter-assay CVs were 4.0 and 6.2%, respectively. Analytical recoveries ranged from 91.3 to 103.6%. Assay detection limit was less than 3 micrograms/l. Linearity of the test is given throughout the measuring range. There was no interference from bilirubin (up to 340 mumol/l), haemoglobin (up to 7 g/l), or rheumatoid factor (up to 1,100 IU/ml). Turbid and lipemic samples interfere. This interference may be avoided by pretreating these samples prior to assay. Results correlated well with those obtained by an automated ELISA test (r = 0.995) and with those of two commercial RIA methods (r greater than 0.97). This latex nephelometric procedure is a convenient method and represents an interesting alternative to other immunoassays for measuring ferritin in human serum.

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Section: Discussionmentioning

confidence: 54%

Automated quantitative nephelometric latex immunoassay for determining ferritin in human serum

Borque

Rus

Cura

et al. 1992

Clinical Laboratory Analysis

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A serum ferritin assay for prevalence studies of iron deficiency

et al. 1986

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A specialized serum ferritin assay has been developed for the detection of iron deficiency in epidemiologic studies. An enzyme immunoassay (EIA) was employed to eliminate the need for radioisotopes. The problem of low sensitivity inherent with the EIA for serum ferritin was eliminated by the use of monoclonal immunologic reagents. The working range of the assay is 1-100 micrograms/L with a sensitivity of 0.5 micrograms/L. Excellent agreement in serum ferritin levels was observed between the present method and the two-site immunoradiometric assay (IRMA), while the variability at low ferritin concentrations was significantly less with the EIA. Because only 10 microliter of serum is required for each assay, duplicate measurements can be performed on a single capillary tube of blood. When an automatic microtiter plate reader for optical density measurements is used, 80-100 duplicate determinations can be completed by one technologist in a single working day.

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